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GTF2A1L Antibody (3E4)

Images

 
Western Blot: GTF2A1L Antibody (3E4) [H00011036-M05] - Analysis of ALF expression in transfected 293T cell line by ALF monoclonal antibody (M05), clone 3E4. Lane 1: ALF transfected lysatE (52.4 KDa). Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: GTF2A1L Antibody (3E4) [H00011036-M05] - Analysis of monoclonal antibody to GTF2A1L on HeLa cell. Antibody concentration 10 ug/ml

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
3E4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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GTF2A1L Antibody (3E4) Summary

Immunogen
ALF (NP_006863, 251 a.a. ~ 348 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PQVSQTNSNVESVLSGSASMAQNLHDESLSTSPHGALHQHVTDIQLHILKNRMYGCDSVKQPRNIEEPSNIPVSEKDSNSQVDLSIRVTDDDIGEIIQ
Localization
Nuclear
Specificity
ALF - TFIIA-alpha/beta-like factor
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GTF2A1L
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GTF2A1L Antibody (3E4)

  • 1-like factor
  • general transcription factor IIA, 1-like
  • GTF2A1LF
  • MGC26254
  • TFIIA large subunit isoform ALF
  • TFIIA-alpha and beta-like factor
  • TFIIA-alpha/beta-like factor

Background

The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involve the effects of TFIIA on the interaction between TATA-binding protein (TBP) and DNA. This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology. Alternative splicing for this locus has been observed and two variants, encoding distinct isoforms, have been identified. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GTF2A1L
Entrez
OMIM
Uniprot