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Rat Gremlin 1 ELISA Kit (Chemiluminescence)

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ELISA: Rat Gremlin 1 ELISA Kit (Chemiluminescence) [NBP2-75364] - Samples were spiked with high concentrations of Rat Gremlin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat Gremlin 1 ELISA Kit (Chemiluminescence) [NBP2-75364] - Samples were spiked with high concentrations of Rat Gremlin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within ...read more
ELISA: Rat Gremlin 1 ELISA Kit (Chemiluminescence) [NBP2-75364] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
31.25-2000 pg/mL
Sensitivity
18.75 pg/mL

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Rat Gremlin 1 ELISA Kit (Chemiluminescence) Summary

Specificity
This kit recognizes Rat GREM1 in samples. No significant cross-reactivity or interference between Rat GREM1 and analogues was observed.
Standard Curve Range
31.25-2000 pg/mL
Sensitivity
18.75 pg/mL
Assay Type
Sandwich-CLIA
Inter-Assay
CV% < 10.37%
Intra-Assay
CV% < 8.91%
Spike Recovery
90-115%
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
GREM1

Applications/Dilutions

Dilutions
  • ELISA
Publications
Read Publication using NBP2-75364.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro CLIA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Substrate Reagent A
  12. Substrate Reagent B

Alternate Names for Rat Gremlin 1 ELISA Kit (Chemiluminescence)

  • Cell proliferation-inducing gene 2 protein
  • CKTSF1B1gremlin 1, cysteine knot superfamily, homolog
  • Cysteine knot superfamily 1, BMP antagonist 1gremlin 1, cysteine knot superfamily, homolog (Xenopus laevis)
  • DAN domain family member 2
  • DAND2
  • DAND2GREMLIN
  • Down-regulated in Mos-transformed cells protein
  • DRM
  • DRMMGC126660
  • GREM1
  • gremlin 1
  • gremlin 1-like protein
  • Gremlin
  • gremlin-1
  • IHG-2
  • Increased in high glucose protein 2
  • increased in high glucose-2
  • proliferation-inducing gene 2

Background

In Xenopus, Gremlin belongs to a novel gene family that act as BMP antagonists in embryonic explants. The human homolog of Drm/Gremlin (CKTFS1B1) is localized on human chromosome 15q13--> q15. DRM-specific mRNA is expressed in different human tissues, including brain, ovary, intestine and colon. It has been suggested that down-regulation of DRM is associated with tumor progression, and support the hypothesis that human DRM may play an important role during both neuroembryological development and carcinogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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  Rat Gremlin 1 ELISA Kit (Chemiluminescence) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol GREM1