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Recombinant Human GDF-7/BMP-12 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Concentration
LYOPH

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Recombinant Human GDF-7/BMP-12 Protein Summary

Description
A disulfide-linked homodimeric protein containing two 129 amino acid chains corresponding to GDF-7/BMP-12 Source: E. coli

Uniprot ID: Q7Z4P5

Amino Acid Sequence: TALAGTRTAQ GSGGGAGRGH GRRGRSRCSR KPLHVDFKEL GWDDWIIAPL DYEAYHCEGL CDFPLRSHLE PTNHAIIQTL LNSMAPDAAP ASCCVPARLS PISILYIDAA NNVVYKQYED MVVEACGCR
Details of Functionality
Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 1.0 ug/ml, corresponding to a specific activity of > 1000 IU/mg.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
GDF7
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 0.1 EU/ug of GDF-7/BMP-12 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant Human GDF-7/BMP-12 Protein

  • BMP12
  • BMP-12
  • GDF7
  • GDF-7
  • growth differentiation factor 7
  • growth/differentiation factor 7

Background

GDF7 encodes a member of the bone morphogenetic protein (BMP) family. BMPs belong to the transforming growth factor-beta superfamily of secreted signalling molecules that regulate diverse processes in growth, repair and embryonic development. In mouse, this gene functions as an inductive signal from the roof plate required for the specification of neuronal identity in the dorsal spinal cord.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol GDF7