Mouse GDF-5/BMP-14 ELISA Kit (Colorimetric) Summary
| Standard Curve Range |
0.16 - 10 ng/mL |
| Sensitivity |
0.09 ng/mL |
| Assay Type |
Sandwich-ELISA |
| Inter-Assay |
%CV < 5.88 |
| Intra-Assay |
%CV < 6.12 |
| Spike Recovery |
88 - 104% |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
GDF5 |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Micro ELISA Plate (Dismountable) - 1 EA, Reference Standard - 2 vials, Concentrated Biotinylated Detection Ab (100X) - 120 uL, Concentrated HRP Conjugate (100X) - 120 uL, Reference Standard & Sample Diluent - 20 mL, Biotinylated Detection Ab Diluent - 14
|
Alternate Names for Mouse GDF-5/BMP-14 ELISA Kit (Colorimetric)
Background
The GDF5 gene is involved in skeletal development through bone and cartilage formation. This gene encodes for a 501 amino acid long, 55 kDA protein that is part of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily which is known to regulate cell growth and differentiation in embryotic and adult tissues. The GDF5 gene is involved in paxillian interactions, mitochondrial apoptosis, the cytokine-cytokine receptor interaction, and the TGF-beta signaling pathway through interacting with other genes such as BMPR1A, BMPR1B, BMPR2, HTRA1, and CHRDL2. The GDF5 gene has been associated to chondrodysplasia, synostosis, periostitis, short stature, cushing's symphalangism, brachydactyly and ankylosis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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Blogs on GDF-5/BMP-14
![ELISA: Mouse GDF-5/BMP-14 ELISA Kit (Colorimetric) [NBP3-08133] - Samples were spiked with high concentrations of Mouse GDF-5/BMP-14 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-08133_mouse-gdf-5-bmp-14-elisa-kit-colorimetric-13220241624346.jpg "ELISA: Mouse GDF-5/BMP-14 ELISA Kit (Colorimetric) [NBP3-08133] - Samples were spiked with high concentrations of Mouse GDF-5/BMP-14 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
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