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GDF-5/BMP-14 Antibody (1065032) [Unconjugated]

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GDF‑5/BMP‑14 was detected in immersion fixed U‑118 MG human glioblastoma/astrocytoma cells (positive) and absent in MCF-7 MG human glioblastoma cells (negative) using Mouse Anti-Human GDF‑5/BMP‑14 Monoclonal Antibody (Catalog # MAB8340) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
GDF‑5/BMP‑14 was detected in immersion fixed U‑118 MG human glioblastoma/astrocytoma cells (positive) and absent in MCF-7 MG human glioblastoma cells (negative) using Mouse Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clone
1065032
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB8340-SP
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GDF-5/BMP-14 Antibody (1065032) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GDF-5
Ala382-Arg501
Accession # P43026
Specificity
Detects human GDF-5 in direct ELISA.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GDF-5/BMP-14 Antibody (1065032) [Unconjugated]

  • BMP14
  • BMP-14
  • Cartilage-derived morphogenetic protein 1
  • cartilage-derived morphogenetic protein-1
  • CDMP-1
  • CDMP1LAP4
  • GDF5
  • GDF-5
  • growth differentiation factor 5
  • growth/differentiation factor 5
  • OS5
  • radotermin
  • SYNS2

Background

Growth Differentiation Factor 5 (GDF-5), also known as cartilage-derived morphogenetic protein 1 (CDMP-1) and BMP-14, is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor  beta (TGF‑ beta ) superfamily. GDF-5 is synthesized as a large precursor protein that consists of an N‑terminal 19 amino acid (aa) signal sequence, a 362 aa pro region and a 120 aa C-terminal mature peptide. Mature GDF-5 is a homodimeric protein which contains the characteristic seven conserved cysteine residues. GDF-5, GDF-6 and GDF-7, which share 80‑86% identity, define a subgroup within the BMP family. Like other TGF‑ beta superfamily proteins, GDF-5 is highly conserved across species. At the amino acid sequence level, mature human and mouse GDF-5 are 98% identical. It has been reported that GDF-5 has multiple functions including regulation of myogenesis, regulation of chondrogenesis, bone morphogenesis, and neuron differentiation and survival. GDF-5 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activation of Smad proteins (Smad 1, 5, and 8).

  1. Storm, E.E. et al. (1994) Nature 368:639.
  2. Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
  3. Francis-West, P.H. et al. (1999) Development 126:1035.
  4. Massague, J. et al. (2000) Genes and Dev. 14:627.
  5. Settle, S.H., Jr. et al. (2003) Dev. Biol. 254:116.
  6. Inada, M. et al. (1996) BBRC 222:317.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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