Home » GATA-5 » GATA-5 Antibodies » GATA-5 Antibody [Unconjugated]
Novus Biologicals products are now on bio-techne.com

GATA-5 Antibody [Unconjugated]

 (1 Publication)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western blot shows lysates of HEK293 human embryonic kidney cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human GATA-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2170) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GATA-5 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HEK293 human embryonic kidney cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human GATA-5 Antigen Affinity-purified ...read more
HeLa human cervical epithelial carcinoma cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GATA‑5/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human ...read more
GATA-5 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human GATA-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2170) at 1.7 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF2170
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2170-SP
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

GATA-5 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GATA-5
Met1-Ala397
Accession # Q9BWX5
Specificity
Detects human GATA-5 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) GATA-1, rhGATA-2, rhGATA-3, rhGATA-4, and rhGATA-6 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GATA5
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using
AF2170 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GATA-5 Antibody [Unconjugated]

  • bB379O24.1
  • GATA binding factor-5
  • GATA binding protein 5
  • GATA5
  • GATA-5
  • GATA-binding factor 5
  • GATA-binding protein 5
  • transcription factor GATA-5

Background

GATA-5 belongs to the GATA family of transcription factors which bind to the consensus DNA sequence (AIT) GATA (AIG) to control diverse tissue-specific programs of gene expression and morphogenesis. GATA5 is important in the development and differentiation of endoderm-derived organs, including heart and gastrointestinal tissues. GATA-5 has also been implicated in cancer development (1, 2).
  1. Tsai, S.F. et al. (1989) Nature 339:446.
  2. Jiang, Y. and T. Evans (1996) Dev. Biol. 174:258.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

GATA-4 Antibody -
BSA Free
NBP2-24585
Immunohistochemistry-Paraffin GATA-4 Antibody - BSA Free
Western Blot GATA-4 Antibody - BSA Free
Immunohistochemistry-Paraffin GATA-4 Antibody - BSA Free
(1 Review)
(2 Publications)
Species: Hu, Mu
Applications: IHC, IHC-P, WB
GATA-6 Antibody
[Unconjugated]
AF1700
Western Blot GATA-6 Antibody [Unconjugated]
Chromatin Immunoprecipitation (ChIP) GATA-6 Antibody [Unconjugated]
Immunocytochemistry GATA-6 Antibody [Unconjugated]
(6 Reviews)
(135 Publications)
Species: Hu
Applications: ChIP, ICC, IHC, Simple Western, WB
QRSL Antibody
NBP1-87992
Western Blot QRSL Antibody
Western Blot QRSL Antibody
Immunohistochemistry-Paraffin QRSL Antibody
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
GATA-4 Antibody -
BSA Free
NBP2-24585
Immunohistochemistry-Paraffin GATA-4 Antibody - BSA Free
Western Blot GATA-4 Antibody - BSA Free
Immunohistochemistry-Paraffin GATA-4 Antibody - BSA Free
(1 Review)
(2 Publications)
Species: Hu, Mu
Applications: IHC, IHC-P, WB
GATA-5 Antibody
[Unconjugated]
AF2170
Western Blot GATA-5 Antibody [Unconjugated]
Chromatin Immunoprecipitation (ChIP) GATA-5 Antibody [Unconjugated]
Immunocytochemistry GATA-5 Antibody [Unconjugated]
(1 Publication)
Species: Hu
Applications: ChIP, ICC, WB
p19ARF/CDKN2A
Antibody - BSA Free
NB200-106
Immunohistochemistry p19ARF/CDKN2A Antibody - BSA Free
Western Blot p19ARF/CDKN2A Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence p19ARF/CDKN2A Antibody - BSA Free
(1 Review)
(48 Publications)
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
NKX2.5 Antibody
[Unconjugated]
AF2444
Western Blot NKX2.5 Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence NKX2.5 Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence NKX2.5 Antibody [Unconjugated]
(2 Reviews)
(13 Publications)
Species: Hu
Applications: IHC, WB
Nodal
[Unconjugated]
3218-ND
Nodal [Unconjugated]
(22 Publications)
Species: Hu
Applications: BA
GATA-6 Antibody
[Unconjugated]
AF1700
Western Blot GATA-6 Antibody [Unconjugated]
Chromatin Immunoprecipitation (ChIP) GATA-6 Antibody [Unconjugated]
Immunocytochemistry GATA-6 Antibody [Unconjugated]
(6 Reviews)
(135 Publications)
Species: Hu
Applications: ChIP, ICC, IHC, Simple Western, WB
GATA-1 Antibody
(4F5) - BSA Free
NBP1-47492
Immunohistochemistry GATA-1 Antibody (4F5) - BSA Free
Immunocytochemistry/ Immunofluorescence GATA-1 Antibody (4F5) - BSA Free
Immunohistochemistry-Paraffin GATA-1 Antibody (4F5) - BSA Free
(1 Review)
(2 Publications)
Species: Pm, Hu
Applications: ChIP, ELISA, ICC/IF, IHC, IHC-P, WB
HNF1 Antibody
NBP1-33596
Western Blot HNF1 Antibody
Western Blot HNF1 Antibody
Immunoprecipitation HNF1 Antibody
(6 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, PAGE, WB
HNF-3 beta/FoxA2
Antibody [Unconjugate ...
AF2400
Chromatin Immunoprecipitation (ChIP) HNF-3 beta/FoxA2 Antibody [Unconjugated]
Immunocytochemistry HNF-3 beta/FoxA2 Antibody [Unconjugated]
Immunohistochemistry HNF-3 beta/FoxA2 Antibody [Unconjugated]
(99 Publications)
Species: Hu
Applications: ChIP, ICC, IHC, WB
SOX17 Antibody
[Unconjugated]
AF1924
Chromatin Immunoprecipitation (ChIP) SOX17 Antibody [Unconjugated]
Simple Western SOX17 Antibody [Unconjugated]
Immunocytochemistry SOX17 Antibody [Unconjugated]
(3 Reviews)
(487 Publications)
Species: Hu
Applications: ChIP, ICC, Simple Western, WB
GATA-3 Antibody
[Unconjugated]
AF2605
Western Blot GATA-3 Antibody [Unconjugated]
Immunocytochemistry GATA-3 Antibody [Unconjugated]
Simple Western GATA-3 Antibody [Unconjugated]
(43 Publications)
Species: Hu
Applications: ICC, IHC, Simple Western, WB
Notch-1 Antibody
(mN1A) - BSA Free
NB100-78486
Immunohistochemistry-Paraffin Notch-1 Antibody (mN1A) - BSA Free
Western Blot Notch-1 Antibody (mN1A) - BSA Free
Flow Cytometry Notch-1 Antibody (mN1A) - BSA Free
(2 Reviews)
(10 Publications)
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, IP, KO, WB
CDX2 Antibody
NBP2-24583
Western Blot CDX2 Antibody
Western Blot CDX2 Antibody
Immunohistochemistry-Paraffin CDX2 Antibody
Species: Bv, Eq, Hu, Mu, Ma-Op, Pm, Rt
Applications: IHC, IHC-P, WB
TCF-2/HNF-1 beta
Antibody
NBP1-89680
Immunocytochemistry/ Immunofluorescence TCF-2/HNF-1 beta Antibody
Immunohistochemistry-Paraffin TCF-2/HNF-1 beta Antibody
Immunohistochemistry-Paraffin TCF-2/HNF-1 beta Antibody
(1 Review)
(7 Publications)
Species: Hu, Mu
Applications: ChIP, ICC/IF, IHC, IHC-P, Simple Western, WB
BMP-4
[Unconjugated]
314-BP
SDS-PAGE BMP-4 [Unconjugated]
Bioactivity BMP-4 [Unconjugated]
(525 Publications)
Species: Hu
Applications: BA, BA
HAND2 Antibody
[Unconjugated]
AF3876
Immunohistochemistry HAND2 Antibody [Unconjugated]
(5 Publications)
Species: Hu, Mu
Applications: IHC, WB
Cadherin-13
Antibody [Unconjugate
AF3264
Immunohistochemistry Cadherin-13 Antibody [Unconjugated]
Immunocytochemistry Cadherin-13 Antibody [Unconjugated]
Simple Western Cadherin-13 Antibody [Unconjugated]
(19 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB

Publications for GATA-5 Antibody (AF2170)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ChIP.


Filter By Application
ChIP
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for GATA-5 Antibody (AF2170) (0)

There are no reviews for GATA-5 Antibody (AF2170). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GATA-5 Antibody (AF2170) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional GATA-5 Products

Blogs on GATA-5

There are no specific blogs for GATA-5, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GATA-5 Antibody [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol GATA5
Uniprot