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NKX2.5 Antibody [Unconjugated]

 (13 Publications)
 (2 Reviews)
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EOMES programs hESCs into functional CMs at high efficiency. a Illustration of growth factor-mediated and optimized EOMES induction-based cardiac differentiation protocols. Bottom right: Immunoblot validating doxycycline-dependent EOMES expression in a transgenic EOMESKO/E.TET-ON hESC line. b Typical yields of hESC-CMs (left, flow cytometry) and NKX2.5 expression (right, immunoblot) obtained with the two protocols (day 10). c Immunostainings 21 days after the initiation of EOMES induction. Weak perinuclear ANP staining is typical in overall MLC2v-positive hESC-CMs. Scale bars: 25 (top) and 50 µm (bottom). d Acceleration and slowdown of spontaneous beat rates in pCMs following exposure to 10 µM isoprenaline and 10 µM propranolol, respectively, on multielectrode arrays. e Microarray-based time course analysis comparing the indicated protocols and cell lines. RESCUE cells carry an inducible EOMES transgene on EOMESKO HuES6 background. Underlying data are from Supplementary Data 2 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29382828), licensed under a CC-BY license. Not internally tested by R&D Systems.
EOMES programs hESCs into functional CMs at high efficiency. a Illustration of growth factor-mediated and optimized EOMES induction-based cardiac differentiation protocols. Bottom right: Immunoblot validating ...read more
Contextual requirements of EOMES-mediated CM programming. a DOX dose dependency of the EOMES TET-ON protocol using the WTE.TET-ON hESC line. Top panel: Immunostains at 1.5 wk. Scale bar: 100 µm. Bottom: Flow ...read more
EOMES knockout hESCs fail to differentiate into cardiomyocytes. a Immunoblot confirming EOMES expression and its absence in WT and KO cells, respectively, at the cardiac mesoderm stage of directed differentiation. b ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF2444
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2444-SP
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NKX2.5 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human NKX2.5
Gln24-Arg137
Accession # P52952
Specificity
Detects human NKX2.5 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NKX2-5
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF2444 in the following applications:
Publications
Read Publications using
AF2444 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKX2.5 Antibody [Unconjugated]

  • cardiac-specific homeo box
  • cardiac-specific homeobox 1
  • Cardiac-specific homeobox
  • CHNG5
  • CSX1
  • CSXFLJ97195
  • FLJ52202
  • FLJ99536
  • Homeobox protein CSX
  • Homeobox protein NK-2 homolog E
  • homeobox protein Nkx-2.5
  • NK2 transcription factor related, locus 5 (Drosophila)
  • NKX2.5
  • NKX2.5FLJ97166
  • NKX2-5
  • NKX2E
  • NKX2EFLJ97197
  • NKX4-1
  • tinman paralog

Background

The homeobox transcriptional factor NKX2.5 (Homeobox protein NK-2 homolog E) specifies cardiac and visceral mesoderm and plays an essential role in heart development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NKX2.5 Antibody (AF2444)(13)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC, IHC, IHC-Fr, IHC-P.


Filter By Application
ICC
(2)
IHC
(1)
IHC-Fr
(2)
IHC-P
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(3)
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF2444 Applications Species
Wai Hoe Ng, Elizabeth K Johnston, Jun Jie Tan, Jacqueline M Bliley, Adam W Feinberg, Donna B Stolz et al. Recapitulating human cardio-pulmonary co-development using simultaneous multilineage differentiation of pluripotent stem cells eLife 1/12/2022 [PMID: 35018887]
W Chen, D Pretorius, Y Zhou, Y Nakada, J Yang, J Zhang TT-10-loaded nanoparticles promote cardiomyocyte proliferation and cardiac repair in a mouse model of myocardial infarction JCI Insight, 2021-10-22;6(20):. 2021-10-22 [PMID: 34676829] (ICC, Mouse) ICC Mouse
Deepe R, Fitzgerald E, Wolters R et al. The Mesenchymal Cap of the Atrial Septum and Atrial and Atrioventricular Septation J Cardiovasc Dev Dis 11/4/2020 [PMID: 33158164]
Tianfang Yang, Zhen Huang, Hua Li, Linyan Wang, YiPing Chen Conjugated activation of myocardial-specific transcription of Gja5 by a pair of Nkx2-5-Shox2 co-responsive elements Developmental Biology 9/1/2020 [PMID: 32687896]
Ayhan Atmanli, Dongjian Hu, Frederik Ernst Deiman, Annebel Marjolein van de Vrugt, François Cherbonneau, Lauren Deems Black et al. Multiplex live single-cell transcriptional analysis demarcates cellular functional heterogeneity eLife 10/8/2019 [PMID: 31591966]
Bertero A, Fields P A et al. Chromatin compartment dynamics in a haploinsufficient model of cardiac laminopathy. J Cell Biol 2/9/2019 [PMID: 31395619]
Inhibiting Extracellular Vesicle Release from Human Cardiosphere Derived Cells with Lentiviral Knockdown of nSMase2 Differentially Effects Proliferation and Apoptosis in Cardiomyocytes, Fibroblasts and Endothelial Cells In Vitro PLoS ONE, 2016-11-02;11(11):e0165926. 2016-11-02 [PMID: 27806113] (IHC-Fr, Mouse) IHC-Fr Mouse
Hongran Wang, Yutao Xi, Yi Zheng, Xiaohong Wang, Austin J. Cooney Generation of electrophysiologically functional cardiomyocytes from mouse induced pluripotent stem cells Stem Cell Research 3/1/2016 [PMID: 26972055]
Nathan J. Palpant, Peter Hofsteen, Lil Pabon, Hans Reinecke, Charles E. Murry Cardiac Development in Zebrafish and Human Embryonic Stem Cells Is Inhibited by Exposure to Tobacco Cigarettes and E-Cigarettes PLOS ONE 5/15/2015 [PMID: 25978043]
Luo J, Weaver M, Cao B, Dennis J, Van Biber B, Laflamme M, Allen M Cobalt protoporphyrin pretreatment protects human embryonic stem cell-derived cardiomyocytes from hypoxia/reoxygenation injury in vitro and increases graft size and vascularization in vivo. Stem Cells Transl Med, 2014-04-15;3(6):734-44. 2014-04-15 [PMID: 24736402] (IHC, IHC-Fr, Human) IHC, IHC-Fr Human
Show All 13 Publications.

Reviews for NKX2.5 Antibody (AF2444) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Other.

Reviews using AF2444:
Filter by Applications
IF
(2)
All Applications
Filter by Species
Mouse
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
 IF Mouse 01/07/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 24123507
SpeciesMouse
  4
reviewed by:
Verified Customer
 IF Other 01/06/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 24123507
SpeciesOther

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Recent Reviews

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1
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 Verified Customer
01/07/2015
Application: IF
Species: Mouse
read this review
 
 Verified Customer
01/06/2015
Application: IF
Species: Other
read this review

Bioinformatics

Gene Symbol NKX2-5
Uniprot