We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

I want to know why GAPDH is used as a loading control antibody in WB techniques.

Our GAPDH antibody makes a good loading control because GAPDH is a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody we already had but it didn't work. What can you recommend?

Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

What secondary antibody should I use for visualization of the GAPDH antibody?

This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

Do you offer a smaller size for the GAPDH antibodies?

We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

For homogenized tissue, beta-actin and GAPDH are both fine.

We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul.

Novus Biologicals products are now on bio-techne.com

GAPDH Antibody [Unconjugated]

Name: GAPDH Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: 2275-PC-100
Price: 409 USD

(105 Publications)

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Western blot shows lysates of U2OS human osteosarcoma cell line, A20 mouse B cell lymphoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.1 µg/mL Rabbit Anti-Human/Mouse/Rat GAPDH Polyclonal ...read more

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, C2C12 mouse myoblast cell line, and C6 rat glioma cell line, loaded at 0.2 mg/mL. A specific band was detected for GAPDH at ...read more

Higher levels of CD4 are found in macrophages expressing LCK.A) Schematic diagram of self-inactivating lentiviral construct used to express wild-type Lck and puroR (LCKWT P). A similar construct was used to express a ...read more

CD4 knock-down in genetically modified stem cell-derived macrophages. C) Detection of protein expression of CD4. Control and transgenic PSC-macrophages lysates were analysed by western blotting using anti-CD4 ...read more

Product Details

Summary

Reactivity	Hu, Mu, RtSpecies Glossary
Applications	WB, Simple Western, IP
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated

Order Details

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GAPDH Antibody [Unconjugated] Summary

Immunogen	Synthetic peptide corresponding to a portion of the human GAPDH/G3PDH sequence
Specificity	Detects human, mouse, and rat GAPDH/G3PDH in Western blots.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	GAPDH
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions

Immunoprecipitation 1:1000-1:5000 dilution
Simple Western 1 ug/mL
Western Blot 0.1 ug/mL

Publications

Read Publications using
2275-PC-100 in the following applications:

Positive Control
1 publication
Western Blot
40 publications

Packaging, Storage & Formulations

Storage
Buffer	Supplied as a solution containg PBS.
Preservative	No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GAPDH Antibody [Unconjugated]

aging-associated gene 9 protein
EC 1.2.1
EC 1.2.1.12
EC 2.6.99.-
G3PD
G3PDH
GAPD
GAPDH
glyceraldehyde 3-phosphate dehydrogenase
glyceraldehyde-3-phosphate dehydrogenase
MGC88685
Peptidyl-cysteine S-nitrosylase GAPDH

Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a 36‑40 kDa member of the GAPDH family of enzymes. It is a widely expressed heterotetramer that is found in both the nucleus and cytoplasm. Although GAPDH was initially identified as a glycolytic enzyme that converted G3P into 1,3 diphosphoglycerate, it is now recognized to participate in no less than endocytosis, membrane fusion, vesicular secretory transport, DNA replication and repair, and apoptosis. Human GAPDH is 335 amino acids (aa) in length and contains two NAD binding sites (Asp35 and Asn316) with a catalytic region between aa 151‑155. GAPDH contains more than 19 posttranslational modifications, including methylation, deamidation and phosphorylation. One splice variant shows a 10 aa substitution for aa 319‑335. Over amino acid 1‑150, human GAPDH shares 92% aa identity with mouse GAPDH.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GAPDH Antibody (2275-PC-100)(105)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Canine, Xenograft.

We have publications tested in 2 applications: Positive Control, Western Blot.

Filter By Application

Positive Control

(1)

Western Blot

(40)

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Human

(32)

Mouse

(8)

Rat

(2)

Canine

(1)

Xenograft

(1)

All Species

Showing Publications 1 - 10 of 105. Show All 105 Publications.

Publications using 2275-PC-100	Applications	Species
Li, Q;Zhang, Q;Kim, YR;Gaddam, RR;Jacobs, JS;Bachschmid, MM;Younis, T;Zhu, Z;Zingman, L;London, B;Rauckhorst, AJ;Taylor, EB;Norris, AW;Vikram, A;Irani, K; Deficiency of endothelial sirtuin1 in mice stimulates skeletal muscle insulin sensitivity by modifying the secretome Nature communications 2023-09-11 [PMID: 37696839] (Western Blot, Human)	Western Blot	Human
J.R. Cox, M. Jennings, C. Lenahan, M. Manion, S. Courville, J. Blazeck Rational engineering of an improved adenosine deaminase 2 enzyme for weaponizing T-cell therapies Immuno-Oncology and Technology 9/1/2023 [PMID: 37519414]
Ota, R;Watanabe, T;Wazawa, Y;Kuwajima, H;Honda, T;Soeda, S;Saito, Y;Yuki, R;Fukumoto, Y;Yamaguchi, N;Yamaguchi, N;Nakayama, Y; v-Src delocalizes Aurora B by suppressing Aurora B kinase activity during monopolar cytokinesis Cellular signalling 2023-06-12 [PMID: 37315749] (Western Blot, Human)	Western Blot	Human
Aneela Nomura, Tetsuro Kobayashi, Wooseok Seo, Michiko Ohno-Oishi, Kiyokazu Kakugawa, Sawako Muroi et al. Identification of a novel enhancer essential for Satb1 expression in TH2 cells and activated ILC2s Life Science Alliance 2023-08-01 [PMID: 37193606]
D Akasaka, S Iguchi, R Kaneko, Y Yoshiga, D Kajiwara, Y Nakachi, N Noma, K Tanaka, A Shimizu, F Hosoi Novel Bruton's tyrosine kinase inhibitor TAS5315 suppresses the progression of inflammation and joint destruction in rodent collagen-induced arthritis PLoS ONE, 2023-02-23;18(2):e0282117. 2023-02-23 [PMID: 36821545] (Positive Control, Human)	Positive Control	Human
VS Atanasova, A Riedl, M Strobl, J Flandorfer, D Unterleuth, C Weindorfer, P Neuhold, S Stang, M Hengstschl, M Bergmann, H Dolznig Selective Eradication of Colon Cancer Cells Harboring PI3K and/or MAPK Pathway Mutations in 3D Culture by Combined PI3K/AKT/mTOR Pathway and MEK Inhibition International Journal of Molecular Sciences, 2023-01-14;24(2):. 2023-01-14 [PMID: 36675180] (Western Blot, Human)	Western Blot	Human
O Morante-Pa, G Godoy-Tena, J Calafell-S, L Ciudad, EM Martínez-C, JL Sardina, E Ballestar Vitamin C enhances NF-kappaB-driven epigenomic reprogramming and boosts the immunogenic properties of dendritic cells Nucleic Acids Research, 2022-10-28;50(19):10981-10994. 2022-10-28 [PMID: 36305821] (Western Blot, Human)	Western Blot	Human
SS Kim, C Doherty, M Moghe, A Rait, KF Pirollo, JB Harford, EH Chang Nanomedicine-Based Gene Delivery for a Truncated Tumor Suppressor RB94 Promotes Lung Cancer Immunity Cancers, 2022-10-18;14(20):. 2022-10-18 [PMID: 36291878] (Western Blot, Xenograft)	Western Blot	Xenograft
GL Lehmann, M Ginsberg, DJ Nolan, C Rodríguez, J Martínez-G, S Zeng, AP Voigt, RF Mullins, S Rafii, E Rodriguez-, I Benedicto Retinal Pigment Epithelium-Secreted VEGF-A Induces Alpha-2-Macroglobulin Expression in Endothelial Cells Cells, 2022-09-24;11(19):. 2022-09-24 [PMID: 36230937] (Western Blot, Human)	Western Blot	Human
T Takikawa, S Hamada, R Matsumoto, Y Tanaka, F Kataoka, A Sasaki, A Masamune Senescent Human Pancreatic Stellate Cells Secrete CXCR2 Agonist CXCLs to Promote Proliferation and Migration of Human Pancreatic Cancer AsPC-1 and MIAPaCa-2 Cell Lines International Journal of Molecular Sciences, 2022-08-17;23(16):. 2022-08-17 [PMID: 36012531] (Western Blot, Human)	Western Blot	Human
T Kuga, N Inoue, K Sometani, S Murataka, M Saraya, R Sugita, T Mikami, Y Takeda, M Taniguchi, K Nishida, N Yamagishi The conserved C-terminal residues of FAM83H are required for the recruitment of casein kinase 1 to the keratin cytoskeleton Scientific Reports, 2022-07-12;12(1):11819. 2022-07-12 [PMID: 35821396] (Western Blot, Human)	Western Blot	Human
P Xiao, T Takiishi, NM Violato, G Licata, F Dotta, G Sebastiani, L Marselli, SP Singh, M Sze, G Van Loo, E Dejardin, EN Gurzov, AK Cardozo NF-kappaB-inducing kinase (NIK) is activated in pancreatic beta-cells but does not contribute to the development of diabetes Cell Death & Disease, 2022-05-19;13(5):476. 2022-05-19 [PMID: 35589698] (Western Blot, Human)	Western Blot	Human
Christopher M. Parry, Li F. Chan, Daniel F. Carr, Daniel B. Hawcutt Platelet-derived growth factor D expression in adrenal cells is modulated by corticosteroids: putative role in adrenal suppression Pediatric Research 2023-01-01 [PMID: 35568735]
Thottakkattumana Parameswaran, V;Hild, C;Eichner, G;Ishaque, B;Rickert, M;Steinmeyer, J; Interleukin-1 Induces the Release of Lubricating Phospholipids from Human Osteoarthritic Fibroblast-like Synoviocytes International journal of molecular sciences [PMID: 35269552]
A Bonfim-Mel, I Noordstra, S Gupta, AH Chan, MJK Jones, K Schroder, AS Yap Rapid lamellipodial responses by neighbor cells drive epithelial sealing in response to pyroptotic cell death Cell Reports, 2022-02-01;38(5):110316. 2022-02-01 [PMID: 35108534] (Western Blot, Human)	Western Blot	Human
Ravinder Reddy Gaddam, Young‐Rae Kim, Julia S. Jacobs, Jin‐Young Yoon, Qiuxia Li, Angela Cai et al. The microRNA‐204‐5p inhibits APJ signalling and confers resistance to cardiac hypertrophy and dysfunction Clinical and Translational Medicine 1/21/2022 [PMID: 35060347]
T Ibata, J Lyu, H Imachi, K Fukunaga, S Sato, T Kobayashi, T Saheki, T Yoshimura, K Murao Effects of 2-Methoxyestradiol, a Main Metabolite of Estradiol on Hepatic ABCA1 Expression in HepG2 Cells Nutrients, 2022-01-11;14(2):. 2022-01-11 [PMID: 35057469] (Western Blot, Human)	Western Blot	Human
O Morante-Pa, L Ciudad, R Micheroli, C de la Call, T Li, G Barbisan, M Houtman, SG Edalat, M Frank-Bert, C Ospelt, E Ballestar Coordinated glucocorticoid receptor and MAFB action induces tolerogenesis and epigenome remodeling in dendritic cells Nucleic Acids Research, 2022-01-11;0(0):. 2022-01-11 [PMID: 34893889] (Western Blot, Human)	Western Blot	Human
Park MK, Zhang L, Min KW et al. NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis Cell metabolism 12/7/2021 [PMID: 34879239]
K Koltowska, KS Okuda, M Gloger, M Rondon-Gal, E Mason, J Xuan, S Dudczig, H Chen, H Arnold, R Skoczylas, NI Bower, S Paterson, AK Lagendijk, GJ Baillie, I Leshchiner, C Simons, KA Smith, W Goessling, JK Heath, RB Pearson, E Sanij, S Schulte-Me, BM Hogan The RNA helicase Ddx21 controls Vegfc-driven developmental lymphangiogenesis by balancing endothelial cell ribosome biogenesis and p53 function Nature Cell Biology, 2021-11-08;23(11):1136-1147. 2021-11-08 [PMID: 34750583] (Human)		Human
Yana Liu, Shihong Zhang, Zhiwei Xue, Xiaoxia Zhou, Lin Tong, Jiachen Liao et al. Bone mesenchymal stem cells-derived miR-223-3p-containing exosomes ameliorate lipopolysaccharide-induced acute uterine injury via interacting with endothelial progenitor cells Bioengineered 2021-12-20 [PMID: 34738867]
Hye Sun Kuehn, Jingjie Chang, Motoi Yamashita, Julie E. Niemela, Chengcheng Zou, Kazuki Okuyama et al. T and B cell abnormalities, pneumocystis pneumonia, and chronic lymphocytic leukemia associated with an AIOLOS defect in patients Journal of Experimental Medicine 2021-12-06 [PMID: 34694366]
RG Feichtinge, A Hüllen, A Koller, D Kotzot, V Grote, E Rapp, P Hofbauer, K Brugger, C Thiel, JA Mayr, SB Wortmann A spoonful of L-fucose-an efficient therapy for GFUS-CDG, a new glycosylation disorder Embo Molecular Medicine, 2021-09-01;13(9):e14332. 2021-09-01 [PMID: 34468083] (Western Blot, Human)	Western Blot	Human
H Morimoto, M Ueno, H Tanabe, T Kono, H Ogawa Progesterone depletion results in Lamin B1 loss and induction of cell death in mouse trophoblast giant cells PLoS ONE, 2021-07-14;16(7):e0254674. 2021-07-14 [PMID: 34260661] (Western Blot, Human)	Western Blot	Human
M Yamashita, HS Kuehn, K Okuyama, S Okada, Y Inoue, N Mitsuiki, K Imai, M Takagi, H Kanegane, M Takeuchi, N Shimojo, M Tsumura, AK Padhi, KYJ Zhang, B Boisson, JL Casanova, O Ohara, SD Rosenzweig, I Taniuchi, T Morio A variant in human AIOLOS impairs adaptive immunity by interfering with IKAROS Nature Immunology, 2021-06-21;22(7):893-903. 6/21/2021 [PMID: 34155405]
R Cheng, X Liu, Z Wang, K Tang ABT?737, a Bcl?2 family inhibitor, has a synergistic effect with apoptosis by inducing urothelial carcinoma cell necroptosis Molecular Medicine Reports, 2021-03-31;23(6):. 2021-03-31 [PMID: 33786632] (Western Blot, Human)	Western Blot	Human
Hui Ding, Jinjun Chen, Jingping Qin, Ruhua Chen, Zili Yi TGF-beta -induced alpha -SMA expression is mediated by C/EBP beta acetylation in human alveolar epithelial cells Molecular Medicine 2021-12-01 [PMID: 33663392]
CE Bullerwell, PP Robichaud, PML Deprez, AP Joy, G Wajnberg, D D'Souza, S Chacko, S Fournier, N Crapoulet, DA Barnett, SM Lewis, RJ Ouellette EBF1 drives hallmark B cell gene expression by enabling the interaction of PAX5 with the MLL H3K4 methyltransferase complex Scientific Reports, 2021-01-15;11(1):1537. 2021-01-15 [PMID: 33452395] (Western Blot, Human)	Western Blot	Human
JK Cullen, GM Boyle, PY Yap, S Elmlinger, JL Simmons, N Broit, J Johns, B Ferguson, LA Maslovskay, AI Savchenko, PM Mirzayans, A Porzelle, PV Bernhardt, VA Gordon, PW Reddell, A Pagani, G Appendino, PG Parsons, CM Williams Activation of PKC supports the anticancer activity of tigilanol tiglate and related epoxytiglianes Scientific Reports, 2021-01-08;11(1):207. 2021-01-08 [PMID: 33420238] (Western Blot, Human)	Western Blot	Human
D Bojkova, M Bechtel, KM McLaughlin, JE McGreig, K Klann, C Bellinghau, G Rohde, D Jonigk, P Braubach, S Ciesek, C Münch, MN Wass, M Michaelis, J Cinatl Aprotinin Inhibits SARS-CoV-2 Replication Cells, 2020-10-30;9(11):. 2020-10-30 [PMID: 33143316] (Western Blot, Human)	Western Blot	Human
Jessica L. Teo, Vanesa M. Tomatis, Luke Coburn, Anne K. Lagendijk, Irin-Maya Schouwenaar, Srikanth Budnar et al. Src kinases relax adherens junctions between the neighbors of apoptotic cells to permit apical extrusion Molecular Biology of the Cell 2020-11-01 [PMID: 32903148]
P González-R, M Cheray, J Füllgrabe, M Salli, P Engskog-Vl, L Keane, V Cunha, A Lupa, W Li, Q Ma, K Dreij, MG Rosenfeld, B Joseph The DNA methyltransferase DNMT3A contributes to autophagy long-term memory Autophagy, 2020-09-14;0(0):1-19. 2020-09-14 [PMID: 32876528] (Western Blot, Human, Mouse)	Western Blot	Human, Mouse
T Kuga, Y Yamane, S Hayashi, M Taniguchi, N Yamaguchi, N Yamagishi Depletion of Csk preferentially reduces the protein level of LynA in a Cbl-dependent manner in cancer cells Sci Rep, 2020-05-06;10(1):7621. 2020-05-06 [PMID: 32376886] (Western Blot, Human)	Western Blot	Human
S Jobbagy, DA Vitturi, SR Salvatore, MF Pires, P Rowart, DR Emlet, M Ross, S Hahn, C St Croix, SG Wendell, AR Subramanya, AC Straub, RJ Tan, FJ Schopfer Nrf2 activation protects against lithium-induced nephrogenic diabetes insipidus JCI Insight, 2020-01-16;5(1):. 2020-01-16 [PMID: 31941842] (Western Blot, Mouse)	Western Blot	Mouse
F Costa Mend, C Duque Lope, R Ricci-Azev, A Sardinha-S, C Figueiredo, AC Paiva Aleg, L L Nohara, A B Carneiro, A Panunto-Ca, I C Almeida, MC Roque-Barr Receptor Heterodimerization and Co-Receptor Engagement in TLR2 Activation Induced by MIC1 and MIC4 from Toxoplasma gondii Int J Mol Sci, 2019-10-10;20(20):. 2019-10-10 [PMID: 31658592] (Western Blot, Mouse)	Western Blot	Mouse
MT Wang, N Fer, J Galeas, EA Collisson, SE Kim, J Sharib, F McCormick Blockade of leukemia inhibitory factor as a therapeutic approach to KRAS driven pancreatic cancer Nat Commun, 2019-07-11;10(1):3055. 2019-07-11 [PMID: 31296870] (Western Blot, Human)	Western Blot	Human
T Saito, N Mihira, Y Matsuba, H Sasaguri, S Hashimoto, S Narasimhan, B Zhang, S Murayama, M Higuchi, VMY Lee, JQ Trojanowsk, TC Saido Humanization of the entire murine Mapt gene provides a murine model of pathological human tau propagation J. Biol. Chem., 2019-07-04;0(0):. 2019-07-04 [PMID: 31273083] (Western Blot, Mouse)	Western Blot	Mouse
X Chang, S Li, XD Xue, F Chang Propranolol regulates ERK1/2 signaling pathway and promotes chronic wound healing in diabetic rats Eur Rev Med Pharmacol Sci, 2019-05-01;23(10):4498-4506. 2019-05-01 [PMID: 31173327] (Western Blot, Rat)	Western Blot	Rat
M Inada, M Shindo, K Kobayashi, A Sato, Y Yamamoto, Y Akasaki, K Ichimura, SI Tanuma Anticancer effects of a non-narcotic opium alkaloid medicine, papaverine, in human glioblastoma cells PLoS ONE, 2019-05-17;14(5):e0216358. 2019-05-17 [PMID: 31100066] (Western Blot, Human)	Western Blot	Human
RC Coll, JR Hill, CJ Day, A Zamoshniko, D Boucher, NL Massey, JL Chitty, JA Fraser, MP Jennings, AAB Robertson, K Schroder MCC950 directly targets the NLRP3 ATP-hydrolysis motif for inflammasome inhibition Nat. Chem. Biol., 2019-05-13;15(6):556-559. 2019-05-13 [PMID: 31086327] (Western Blot, Human)	Western Blot	Human
RMJ Genga, EM Kernfeld, KM Parsi, TJ Parsons, MJ Ziller, R Maehr Single-Cell RNA-Sequencing-Based CRISPRi Screening Resolves Molecular Drivers of Early Human Endoderm Development Cell Rep, 2019-04-16;27(3):708-718.e10. 2019-04-16 [PMID: 30995470] (Western Blot, Human)	Western Blot	Human
H Nakazawa, K Ikeda, S Shinozaki, S Yasuhara, YM Yu, JAJ Martyn, RG Tompkins, T Yorozu, S Inoue, M Kaneki Coenzyme Q10 protects against burn-induced mitochondrial dysfunction and impaired insulin signaling in mouse skeletal muscle FEBS Open Bio, 2019-01-19;9(2):348-363. 2019-01-19 [PMID: 30761259] (Western Blot, Mouse)	Western Blot	Mouse
Hailan Zhao, Ruiqing Wang, Mingxia Ye, Lan Zhang Genipin protects against H2O2-induced oxidative damage in�retinal pigment epithelial cells by promoting Nrf2 signaling International Journal of Molecular Medicine 2018-12-12 [PMID: 30569096]
SM Brunner, A Koller, J Stockinger, F Locker, S Leis, F Ernst, P Strasser, B Brodowicz, S Ebner, BS Holub, I Rauch, K Graf, R Lang, B Kofler Validation of antibody-based tools for galanin research Peptides, 2018-09-06;120(0):170009. 2018-09-06 [PMID: 30196126] (Western Blot, Human, Mouse)	Western Blot	Human, Mouse
Guangye Han, Xinjun Zhang, Pei Liu, Quanfeng Yu, Zeyu Li, Qinnan Yu et al. Knockdown of anti-silencing function 1B histone chaperone induces cell apoptosis via repressing PI3K/Akt pathway in prostate cancer International Journal of Oncology 2018-08-16 [PMID: 30132513]
SJ Thygesen, KE Takizawa, AAB Robertson, DP Sester, KJ Stacey Compromised NLRP3 and AIM2 inflammasome function in autoimmune NZB/W F1 mouse macrophages Immunol. Cell Biol., 2018-08-19;0(0):. 8/19/2018 [PMID: 30052286]
KG Blecharz-L, V Prinz, M Burek, D Frey, T Schenkel, SM Krug, M Fromm, P Vajkoczy Gelatinolytic activity of autocrine matrix metalloproteinase-9 leads to endothelial de-arrangement in Moyamoya disease J. Cereb. Blood Flow Metab., 2018-04-10;0(0):271678X187684. 2018-04-10 [PMID: 29633884] (Western Blot, Human)	Western Blot	Human
Vitaliya Sagulenko, Nazarii Vitak, Parimala R. Vajjhala, James E. Vince, Katryn J. Stacey Caspase-1 Is an Apical Caspase Leading to Caspase-3 Cleavage in the AIM2 Inflammasome Response, Independent of Caspase-8 Journal of Molecular Biology 2018-01-01 [PMID: 29100888]
Cerqueira D, Bodnar A, Phua Y, Freer R, Hemker S, Walensky L, Hukriede N, Ho J Bim gene dosage is critical in modulating nephron progenitor survival in the absence of microRNAs during kidney development. FASEB J, 2017-04-26;31(8):3540-3554. 2017-04-26 [PMID: 28446592]
Machitani M, Sakurai F, Wakabayashi K, Nakatani K, Tachibana M, Mizuguchi H MicroRNA miR-27 Inhibits Adenovirus Infection by Suppressing the Expression of SNAP25 and TXN2. J Virol, 2017-05-26;91(12):. 2017-05-26 [PMID: 28356525]
Sakagami H, Masuda Y, Tomomura M, Yokose S, Uesawa Y, Ikezoe N, Asahara D, Takao K, Kanamoto T, Terakubo S, Kagaya H, Nakashima H, Sugita Y Quantitative Structure-Cytotoxicity Relationship of Chalcones. Anticancer Res, 2017-03-01;37(3):1091-1098. 2017-03-01 [PMID: 28314269]
Sakagami H, Okudaira N, Masuda Y, Amano O, Yokose S, Kanda Y, Suguro M, Natori T, Oizumi H, Oizumi T Induction of Apoptosis in Human Oral Keratinocyte by Doxorubicin. Anticancer Res, 2017-03-01;37(3):1023-1029. 2017-03-01 [PMID: 28314260]
T McGarry, M Biniecka, W Gao, D Cluxton, M Canavan, S Wade, S Wade, L Gallagher, C Orr, DJ Veale, U Fearon Resolution of TLR2-induced inflammation through manipulation of metabolic pathways in Rheumatoid Arthritis Sci Rep, 2017-02-22;7(0):43165. 2017-02-22 [PMID: 28225071]
Kumar S, Kim Y, Vikram A, Naqvi A, Li Q, Kassan M, Kumar V, Bachschmid M, Jacobs J, Kumar A, Irani K Sirtuin1-regulated lysine acetylation of p66Shc governs diabetes-induced vascular oxidative stress and endothelial dysfunction. Proc Natl Acad Sci U S A, 2017-01-30;114(7):1714-1719. 2017-01-30 [PMID: 28137876]
Venugopal S, Martinez-Arguelles D, Chebbi S, Hullin-Matsuda F, Kobayashi T, Papadopoulos V Plasma Membrane Origin of the Steroidogenic Pool of Cholesterol Used in Hormone-induced Acute Steroid Formation in Leydig Cells. J Biol Chem, 2016-11-03;291(50):26109-26125. 2016-11-03 [PMID: 27815506]
Kamada Y, Kida S, Hirano K, Yamaguchi S, Suzuki A, Hashimoto C, Kimura A, Sato M, Fujii H, Sobajima T, Yamamoto A, Ebisutani Y, Takamatsu S, Shinzaki S, Yoshida Y, Yamada M, Nagasaka H, Takehara T, Miyoshi E Hepatic aberrant glycosylation by N-acetylglucosaminyltransferase V accelerates HDL assembly. Am J Physiol Gastrointest Liver Physiol, 2016-09-22;311(5):G859-G868. 2016-09-22 [PMID: 27659420]
Song R, Tolentino R, Sobie E, Neves-Zaph S Cross-regulation of Phosphodiesterase 1 and Phosphodiesterase 2 Activities Controls Dopamine-mediated Striatal alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptor Trafficking. J Biol Chem, 2016-09-07;291(44):23257-23267. 2016-09-07 [PMID: 27605670]
Tomikoshi Y, Nomura M, Okudaira N, Sakagami H, Wakabayashi H Enhancement of Cytotoxicity of Three Apoptosis-inducing Agents Against Human Oral Squamous Cell Carcinoma Cell Line by Benzoxazinotropone. In Vivo, 2016-09-10;30(5):645-50. 2016-09-10 [PMID: 27566085]
Kim W, Yun S, Park C, Bauer S, Kim J, Lee M, Kim H Sustained Mutant KIT Activation in the Golgi Complex Is Mediated by PKC-theta in Gastrointestinal Stromal Tumors. Clin Cancer Res, 2016-07-20;23(3):845-856. 2016-07-20 [PMID: 27440273]
Mitchell S. Stark, Lisa N. Tom, Glen M. Boyle, Vanessa F. Bonazzi, H. Peter Soyer, Adrian C. Herington et al. The ‘melanoma-enriched’ microRNA miR-4731-5p acts as a tumour suppressor Oncotarget 2016-08-02 [PMID: 27331623]
Daneasa A, Cucolas C, Lenghel L, Olteanu D, Orasan R, Filip G Letrozole vs estradiol valerate induced PCOS in rats: glycemic, oxidative and inflammatory status assessment. Reproduction, 2016-01-20;151(4):401-9. 2016-01-20 [PMID: 26792865]
Watari K, Shibata T, Nabeshima H et al. Impaired differentiation of macrophage lineage cells attenuates bone remodeling and inflammatory angiogenesis in Ndrg1 deficient mice. Sci Rep. 1/18/2016 [PMID: 26778110] Details: RANK antibody used for WB on BM-derived macrophages (BMDMs) from WT and KO mice at a dilution of 1:1000 (Figure 3h).
Constantin L, Constantin M, Wainwright B MicroRNA Biogenesis and Hedgehog-Patched Signaling Cooperate to Regulate an Important Developmental Transition in Granule Cell Development. Genetics, 2016-01-15;202(3):1105-18. 2016-01-15 [PMID: 26773048]
Ariffin J, das Gupta K, Kapetanovic R, Iyer A, Reid R, Fairlie D, Sweet M Histone Deacetylase Inhibitors Promote Mitochondrial Reactive Oxygen Species Production and Bacterial Clearance by Human Macrophages. Antimicrob Agents Chemother, 2015-12-28;60(3):1521-9. 2015-12-28 [PMID: 26711769]
Arnez K, Kindlova M, Bokil N, Murphy J, Sweet M, Guncar G Analysis of the N-terminal region of human MLKL, as well as two distinct MLKL isoforms, reveals new insights into necroptotic cell death. Biosci Rep, 2015-12-24;36(1):e00291. 2015-12-24 [PMID: 26704887]
Li H, Wu X, Hou S, Malek M, Kielkowska A, Noh E, Makondo K, Du Q, Wilkins J, Johnston J, Gibson S, Lin F, Marshall A Phosphatidylinositol-3,4-Bisphosphate and Its Binding Protein Lamellipodin Regulate Chemotaxis of Malignant B Lymphocytes. J Immunol, 2015-12-22;196(2):586-95. 2015-12-22 [PMID: 26695371]
Backlund M, Paukku K, Kontula K, Lehtonen J Endoplasmic reticulum stress increases AT1R mRNA expression via TIA-1-dependent mechanism. Nucleic Acids Res, 2015-12-17;0(0):. 2015-12-17 [PMID: 26681690] (Western Blot, Human)	Western Blot	Human
Phua Y, Chu J, Marrone A, Bodnar A, Sims-Lucas S, Ho J Renal stromal miRNAs are required for normal nephrogenesis and glomerular mesangial survival. Physiol Rep, 2015-10-01;3(10):. 2015-10-01 [PMID: 26438731]
Sanchez E, Bergareche A, Krebs C, Gorostidi A, Makarov V, Ruiz-Martinez J, Chorny A, Lopez de Munain A, Marti-Masso J, Paisan-Ruiz C SORT1 Mutation Resulting in Sortilin Deficiency and p75(NTR) Upregulation in a Family With Essential Tremor. ASN Neuro, 2015-08-21;7(4):. 2015-08-21 [PMID: 26297037]
Feichtinger R, Weis S, Mayr J, Zimmermann F, Bogner B, Sperl W, Kofler B Alterations of oxidative phosphorylation in meningiomas and peripheral nerve sheath tumors. Neuro Oncol, 2015-06-23;18(2):184-94. 2015-06-23 [PMID: 26106125]
Desnoyers G, Frost L, Courteau L, Wall M, Lewis S Decreased eIF3e Expression Can Mediate Epithelial-to-Mesenchymal Transition through Activation of the TGFbeta Signaling Pathway. Mol Cancer Res, 2015-06-08;13(10):1421-30. 2015-06-08 [PMID: 26056130]
Kizu T, Yoshida Y, Furuta K, Ogura S, Egawa M, Chatani N, Hamano M, Takemura T, Ezaki H, Kamada Y, Nishida K, Nakaoka Y, Kiso S, Takehara T Loss of Gab1 adaptor protein in hepatocytes aggravates experimental liver fibrosis in mice. Am J Physiol Gastrointest Liver Physiol, 2015-01-23;308(7):G613-24. 2015-01-23 [PMID: 25617348]
Yeo J, Wall A, Luo L, Stow J Rab31 and APPL2 enhance FcgammaR-mediated phagocytosis through PI3K/Akt signaling in macrophages. Mol Biol Cell, 2015-01-07;26(5):952-65. 2015-01-07 [PMID: 25568335]
DENG HE, SHAOGANG WANG, ZHAOHUI JIA, LEI CUI, YUCHAO LU, HENGLONG HU et al. Calcium ions promote primary renal epithelial cell differentiation into cells with bone-associated phenotypes via transforming growth factor-beta 1-induced epithelial-mesenchymal transition in idiopathic hypercalciuria patients Molecular Medicine Reports 2015-03-01 [PMID: 25394514]
Oda Y, Otani T, Ikenouchi J, Furuse M Tricellulin regulates junctional tension of epithelial cells at tricellular contacts through Cdc42. J Cell Sci, 2014-08-05;127(0):4201-12. 2014-08-05 [PMID: 25097232]
Ninagawa S, Okada T, Sumitomo Y, Kamiya Y, Kato K, Horimoto S, Ishikawa T, Takeda S, Sakuma T, Yamamoto T, Mori K EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step. J Cell Biol, 2014-08-04;206(3):347-56. 2014-08-04 [PMID: 25092655]
Okudaira N, Ishizaka Y, Nishio H Retrotransposition of long interspersed element 1 induced by methamphetamine or cocaine. J Biol Chem, 2014-07-22;289(37):25476-85. 2014-07-22 [PMID: 25053411]
Higurashi T, Endo H, Uchiyama T, Uchiyama S, Yamada E, Ohkubo H, Sakai E, Takahashi H, Maeda S, Wada K, Natsumeda Y, Hippo Y, Nakajima A, Nakagama H Conditional knockout of the leptin receptor in the colonic epithelium revealed the local effects of leptin receptor signaling in the progression of colonic tumors in mice. Carcinogenesis, 2014-06-23;35(9):2134-41. 2014-06-23 [PMID: 24958593]
Hara S, Takano T, Fujikawa T, Yamada M, Wakai T, Kono T, Obata Y Forced expression of DNA methyltransferases during oocyte growth accelerates the establishment of methylation imprints but not functional genomic imprinting. Hum Mol Genet, 2014-03-05;23(14):3853-64. 2014-03-05 [PMID: 24599402]
Villarreal G, Chatterjee A, Oh S, Oh D, Rhee D Pharmacological regulation of SPARC by lovastatin in human trabecular meshwork cells. Invest Ophthalmol Vis Sci, 2014-03-19;55(3):1657-65. 2014-03-19 [PMID: 24474275]
Han , Siew Pin, Gambin , Yann, Gomez , Guillerm, Verma , Suzie, Giles , Nichole, Michael , Magdalen, Wu , Selwin K, Guo , Zhong, Johnston , Wayne, Sierecki , Emma, Parton , Robert G, Alexandrov , Kirill, Yap , Alpha S Cortactin scaffolds Arp2/3 and WAVE2 at the epithelial zonula adherens. J Biol Chem, 2014-01-27;289(11):7764-75. 2014-01-27 [PMID: 24469447] (Western Blot, Human)	Western Blot	Human
van Wilgenburg , Bonnie, Moore , Michael, James , William, Cowley , Sally A The productive entry pathway of HIV-1 in macrophages is dependent on endocytosis through lipid rafts containing CD4. PLoS ONE, 2014-01-22;9(1):e86071. 2014-01-22 [PMID: 24465876] (Western Blot, Human)	Western Blot	Human
Barnett TC, Liebl D, Seymour LM et al. The Globally Disseminated M1T1 Clone of Group A Streptococcus Evades Autophagy for Intracellular Replication. Cell Host Microbe. 12/11/2013 [PMID: 24331465]
Liao C, Jeng C, Wang H, Wang S, Wu J Lipopolysaccharide induces degradation of connexin43 in rat astrocytes via the ubiquitin-proteasome proteolytic pathway. PLoS ONE, 2013-11-13;8(11):e79350. 2013-11-13 [PMID: 24236122] (Western Blot, Rat)	Western Blot	Rat
Brown K, Perry H, Lau J, Jones D, Pulliam J, Thornhill B, Crabtree C, Luo H, Chen Y, Dasgupta P Nicotine induces the up-regulation of the alpha7-nicotinic receptor (alpha7-nAChR) in human squamous cell lung cancer cells via the Sp1/GATA protein pathway. J Biol Chem, 2013-10-02;288(46):33049-59. 2013-10-02 [PMID: 24089524]
Shane Stegeman, Lachlan A. Jolly, Susitha Premarathne, Jozef Gecz, Linda J. Richards, Alan Mackay-Sim et al. Loss of Usp9x Disrupts Cortical Architecture, Hippocampal Development and TGF beta -Mediated Axonogenesis PLoS ONE 2013-07-05 [PMID: 23861879]
Kim Y, Jeung H, Jeong I, Lee K, Rha S, Chung H, Kim G Mechanism of enhancement of radiation-induced cytotoxicity by sorafenib in colorectal cancer. J Radiat Res, 2012-08-24;54(1):52-60. 2012-08-24 [PMID: 22923745]
Siroky B, Yin H, Babcock J, Lu L, Hellmann A, Dixon B, Quilliam L, Bissler J Human TSC-associated renal angiomyolipoma cells are hypersensitive to ER stress. Am J Physiol Renal Physiol, 2012-07-11;303(6):F831-44. 2012-07-11 [PMID: 22791333]
Kakiya N, Saito T, Nilsson P, Matsuba Y, Tsubuki S, Takei N, Nawa H, Saido T Cell surface expression of the major amyloid-beta peptide (Abeta)-degrading enzyme, neprilysin, depends on phosphorylation by mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK) and dephosphorylation by protein phosphatase 1a. J Biol Chem, 2012-07-05;287(35):29362-72. 2012-07-05 [PMID: 22767595]
Aghazadeh Y, Rone M, Blonder J, Ye X, Veenstra T, Hales D, Culty M, Papadopoulos V Hormone-induced 14-3-3gamma adaptor protein regulates steroidogenic acute regulatory protein activity and steroid biosynthesis in MA-10 Leydig cells. J Biol Chem, 2012-03-16;287(19):15380-94. 2012-03-16 [PMID: 22427666]
Fan J, Papadopoulos V Transcriptional regulation of translocator protein (Tspo) via a SINE B2-mediated natural antisense transcript in MA-10 Leydig cells. Biol Reprod, 2012-05-10;86(5):147, 1-15. 2012-05-10 [PMID: 22378763]
Paukku K, Backlund M, de Boer R, Kalkkinen N, Kontula K, Lehtonen J Regulation of AT1R expression through HuR by insulin. Nucleic Acids Res, 2012-02-23;40(12):5250-61. 2012-02-23 [PMID: 22362742]
Kamada Y, Mori K, Matsumoto H, Kiso S, Yoshida Y, Shinzaki S, Hiramatsu N, Ishii M, Moriwaki K, Kawada N, Takehara T, Miyoshi E N-Acetylglucosaminyltransferase V regulates TGF-beta response in hepatic stellate cells and the progression of steatohepatitis. Glycobiology, 2012-01-30;22(6):778-87. 2012-01-30 [PMID: 22294551]
Pearen M, Eriksson N, Fitzsimmons R, Goode J, Martel N, Andrikopoulos S, Muscat G The nuclear receptor, Nor-1, markedly increases type II oxidative muscle fibers and resistance to fatigue. Mol Endocrinol, 2012-01-26;26(3):372-84. 2012-01-26 [PMID: 22282471]
Ni M, MacFarlane A, Toft M, Lowell C, Campbell K, Hamerman J B-cell adaptor for PI3K (BCAP) negatively regulates Toll-like receptor signaling through activation of PI3K. Proc Natl Acad Sci U S A, 2011-12-20;109(1):267-72. 2011-12-20 [PMID: 22187458]
Fuchs C, Rosner M, Dolznig H, Mikula M, Kramer N, Hengstschlager M Tuberin and PRAS40 are anti-apoptotic gatekeepers during early human amniotic fluid stem-cell differentiation. Hum Mol Genet, 2011-11-16;21(5):1049-61. 2011-11-16 [PMID: 22090422]
Kim W, Park M, Kim Y, Tae Y, Yang H, Lee J, Kim H MicroRNA-494 downregulates KIT and inhibits gastrointestinal stromal tumor cell proliferation. Clin Cancer Res, 2011-10-31;17(24):7584-94. 2011-10-31 [PMID: 22042971]
Yang W, Yamada M, Tamura Y, Chang K, Mao J, Zou L, Feng Y, Kida K, Scherrer-Crosbie M, Chao W, Ichinose F, Yu Y, Fischman A, Tompkins R, Yao S, Kaneki M Farnesyltransferase inhibitor FTI-277 reduces mortality of septic mice along with improved bacterial clearance. J Pharmacol Exp Ther, 2011-08-26;339(3):832-41. 2011-08-26 [PMID: 21873557]
Raposo RA, Trudgian DC, Thomas B, van Wilgenburg B, Cowley SA, James W Protein kinase C and NF-kappaB-dependent CD4 downregulation in macrophages induced by T cell-derived soluble factors: consequences for HIV-1 infection. J. Immunol., 2011-06-10;187(2):748-59. 2011-06-10 [PMID: 21666058] (Western Blot, Human)	Western Blot	Human
Villani RM, Adolphe C, Palmer J, Waters MJ, Wainwright BJ Patched1 inhibits epidermal progenitor cell expansion and basal cell carcinoma formation by limiting Igfbp2 activity. Cancer Prev Res (Phila), 2010-09-21;3(10):1222-34. 2010-09-21 [PMID: 20858761] (Western Blot, Mouse)	Western Blot	Mouse
den Elzen N, Buttery CV, Maddugoda MP, Ren G, Yap AS Cadherin adhesion receptors orient the mitotic spindle during symmetric cell division in mammalian epithelia. Mol. Biol. Cell, 2009-06-24;20(16):3740-50. 2009-06-24 [PMID: 19553471] (Western Blot, Canine)	Western Blot	Canine
Taka E, Errahali YJ, Abonyo BO, Bauer DM, Heiman AS Post-transcriptional silencing of CCR3 downregulates IL-4 stimulated release of eotaxin-3 (CCL26) and other CCR3 ligands in alveolar type II cells. Cytokine, 2008-11-26;44(3):342-51. 2008-11-26 [PMID: 19038554] (Western Blot, Human)	Western Blot	Human
Maillard CM, Bouquet C, Petitjean MM, Mestdagt M, Frau E, Jost M, Masset AM, Opolon PH, Beermann F, Abitbol MM, Foidart JM, Perricaudet MJ, Noel AC Reduction of brain metastases in plasminogen activator inhibitor-1-deficient mice with transgenic ocular tumors. Carcinogenesis, 2008-08-27;29(11):2236-42. 2008-08-27 [PMID: 18753414] (Western Blot, Mouse)	Western Blot	Mouse
Abonyo BO, Lebby KD, Tonry JH, Ahmad M, Heiman AS Modulation of eotaxin-3 (CCL26) in alveolar type II epithelial cells. Cytokine, 2007-03-09;36(5):237-44. 2007-03-09 [PMID: 17350279]
Gotoh T, Oyadomari S, Mori K, Mori M Nitric oxide-induced apoptosis in RAW 264.7 macrophages is mediated by endoplasmic reticulum stress pathway involving ATF6 and CHOP. J. Biol. Chem., 2002-01-22;277(14):12343-50. 2002-01-22 [PMID: 11805088]
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FAQs for GAPDH Antibody (2275-PC-100). (Showing 1 - 7 of 7 FAQs).

We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?
- GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.
I want to know why GAPDH is used as a loading control antibody in WB techniques.
- Our GAPDH antibody makes a good loading control because GAPDH is a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.
I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?
- This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.
I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody we already had but it didn't work. What can you recommend?
- Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.
What secondary antibody should I use for visualization of the GAPDH antibody?
- This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.
Do you offer a smaller size for the GAPDH antibodies?
- We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.
I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.
- For homogenized tissue, beta-actin and GAPDH are both fine.

Secondary Antibodies

Isotype Controls

Additional GAPDH Products

Blogs on GAPDH. Showing 1-10 of 13 blog posts - Show all blog posts.

Check out the latest blog posts on GAPDH.

Considerations for Quantitative Western blotting
By Jamshed Arslan, Pharm. D., PhD. Since its inception in 1979, Western blotting has undergone several developments. The use of radioactive probes was common throughout 1980s, but utilizing secondary antibodies labell... Read full blog post.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs. Actin has six highly conserved isoforms, however beta and gamma actin are the two... Read full blog post.

Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p... Read full blog post.

GAPDH - A "Housekeeping" Gene With Diverse Functions in Cellular Homeostasis
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a well-known housekeeping gene with functions in glycolysis. Many biologists are familiar with the gene and use GAPDH antibodies for a loading control when performing western blots. However, this ... Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi... Read full blog post.

GAPDH: More than a housekeeping gene
GAPDH is a 146kD tetramer glycolytic pathway metabolic enzyme composed of four 30-40 kDa subunits. It is responsible for reversibly phosphorylating its substrate glyceraldehyde 3-phosphate within the glycolytic pathway. Apart from its role in gl... Read full blog post.

GAPDH (Glyceraldehyde 3-Phosphate Dehydrogenase)
GAPDH is a 146 kD tetramer glycolytic pathway metabolic enzyme responsible for reversibly phosphorylating glyceraldehyde 3-phosphate. It may have other possible functions in transcriptional activation. GAPDH is highly expressed due to this housekeepin... Read full blog post.

Time to Start Actin Like a Reliable 'Housekeeper'!
A growing body of data and studies using actin antibodies supports a view of the actin cytoskeleton of smooth muscle cells as a dynamic structure that plays an integral role in regulating the development of mechanical tension and the material properti... Read full blog post.

Beta Actin and GAPDH: The Importance of Western Blot Loading Controls
What are Western Blot Loading Controls?Western blotting is an essential technique to probe protein expression in complex cell or tissue lysates. To accurately determine protein expression and interpret Western blot results, it is important to use... Read full blog post.

The GAPDH Antibody in Western Blot Assays
The loading controls on our antibody database are widely used in gel electrophoresis and Western blotting studies. Products like the GAPDH antibody detect "housekeeping" proteins which are abundantly distributed in cells. This makes them useful for ch... Read full blog post.

Showing 1-10 of 13 blog posts - Show all blog posts.

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GAPDH

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

GAPDH Antibody [Unconjugated]

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Publications for GAPDH Antibody (2275-PC-100)(105)

Reviews for GAPDH Antibody (2275-PC-100) (0)

Product General Protocols

Video Protocols

FAQs for GAPDH Antibody (2275-PC-100). (Showing 1 - 7 of 7 FAQs).

Secondary Antibodies

Isotype Controls

Additional GAPDH Products

Blogs on GAPDH. Showing 1-10 of 13 blog posts - Show all blog posts.

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