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Fumarase Antibody

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Genetic Strategies: Western Blot: Fumarase Antibody [NBP1-32893] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ...read more
Immunocytochemistry/ Immunofluorescence: Fumarase Antibody [NBP1-32893] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Fumarase Antibody [NBP1-32893] - Paraffin-embedded OVCAR3 xenograft, using antibody at 1:500 dilution.
Western Blot: Fumarase Antibody [NBP1-32893] - Mouse liver lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.
Western Blot: Fumarase Antibody [NBP1-32893] - Rat liver lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:5000.
Western Blot: Fumarase Antibody [NBP1-32893] - Sample (30 ug of whole cell lysate) A: 293T B: A431 10% SDS PAGE; antibody diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: Fumarase Antibody [NBP1-32893] - Fumarase antibody [N2C2], Internal detects Fumarase protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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Fumarase Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Fumarase. The exact sequence is proprietary.
Localization
Mitochondrion, Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FH
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for Fumarase Antibody

  • EC 4.2.1.2
  • fumarase
  • fumarate hydratase
  • fumarate hydratase, mitochondrial
  • HLRCC
  • LRCC
  • MCL
  • MCUL1

Background

The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FH