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Fumarase Antibody (S08-3F2)

Images

 
Western Blot: Fumarase Antibody (S08-3F2) [NBP3-15029] - Western blot detection of Fumarase in CHO-K1, C6, Jurkat cell lysates using Fumarase Rabbit mAb (1:500 diluted). Predicted band size: 55kDa. Observed band size: ...read more
Immunocytochemistry/ Immunofluorescence: Fumarase Antibody (S08-3F2) [NBP3-15029] - Immunofluorescence of Fumarase (green) in hela using Fumarase Rabbit mAb at dilution 1/50, and DAPI (blue)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clone
S08-3F2
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Fumarase Antibody (S08-3F2) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide of human Fumarase (Uniprot#: P07954)
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
FH
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1/20
  • Western Blot 1/1000-1/2000
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Purity
Affinity purified

Alternate Names for Fumarase Antibody (S08-3F2)

  • EC 4.2.1.2
  • fumarase
  • fumarate hydratase
  • fumarate hydratase, mitochondrial
  • HLRCC
  • LRCC
  • MCL
  • MCUL1

Background

Fumarase is encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Fumarase Antibody (NBP3-15029) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FH