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F4/80 Antibody (521204) [Unconjugated]

Images

 
RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse F4/80/EMR1 Monoclonal Antibody (Catalog # MAB5580, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed ...read more
F4/80 was detected in unfixed live cells fixed RAW 264.7 mouse monocyte/macrophage cell line using Rat Anti-Mouse F4/80 Monoclonal Antibody (Catalog # MAB5580) at 8 µg/mL for 3 hours at room temperature. ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, CyTOF-ready, ICC/IF
Clone
521204
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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F4/80 Antibody (521204) [Unconjugated] Summary

Immunogen
NS0 mouse myeloma cell line transfected with mouse F4/80/EMR1
Gln28-Gly931
Accession # Q61549
Specificity
Detects mouse F4/80/EMR1.
Details of Functionality
None<br /><br />None<br /><br />
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
EMR1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB5580 in the following applications:

Publications
Read Publications using
MAB5580 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for F4/80 Antibody (521204) [Unconjugated]

  • ADGRE1
  • cell surface glycoprotein F4/80
  • DD7A5-
  • DD7A5-7
  • EGF-like module containing, mucin-like, hormone receptor-like sequence 1
  • EGF-like module receptor 1
  • EGF-like module-containing mucin-like hormone receptor-like 1
  • EGF-TM7
  • Emr
  • EMR1 hormone receptor
  • Emr1
  • F4/80
  • Gpf480
  • Ly7
  • Ly71
  • lymphocyte antigen 71
  • Mouse EMR1
  • Rat EMR1
  • TM7L
  • TM7LN3

Background

F4/80, also known as EMR1, is a 160 kDa extensively glycosylated protein in the EGF-TM7 family of adhesion molecules. It is primarily expressed on macrophages and dendritic cells in mouse but is restricted to eosinophils in human. F4/80 plays a role in the development of peripheral tolerance.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for F4/80 Antibody (MAB5580)(22)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P.


Filter By Application
Flow Cytometry
(2)
ICC
(2)
IHC
(7)
IHC-Fr
(3)
IHC-P
(3)
All Applications
Filter By Species
Mouse
(17)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using MAB5580 Applications Species
Y Zhang, R Sang, J Bao, Z Jiang, D Qian, Y Zhou, W Su, J Wei, L Zhao, Z Wei, Y Zhao, M Shi, G Chen Schwann cell-derived CXCL2 contributes to cancer pain by modulating macrophage infiltration in a mouse breast cancer model Brain, Behavior, and Immunity, 2023-02-06;0(0):. 2023-02-06 [PMID: 36754246] (IHC, Mouse) IHC Mouse
Bo-Yoon Park, Dirong Wu, Kyoo-Ri Kwon, Mi-Jin Kim, Tae-Gon Kim, Jun-Ho Lee et al. Implantation and tracing of green fluorescent protein-expressing adipose-derived stem cells in peri-implant capsular fibrosis Stem Cell Research & Therapy 2/8/2023 [PMID: 36750973]
MM Xu, JY Kang, S Ji, YY Wei, SL Wei, JJ Ye, YG Wang, JL Shen, HM Wu, GH Fei Melatonin Suppresses Macrophage M1 Polarization and ROS-Mediated Pyroptosis via Activating ApoE/LDLR Pathway in Influenza A-Induced Acute Lung Injury Oxidative Medicine and Cellular Longevity, 2022-11-15;2022(0):2520348. 2022-11-15 [PMID: 36425057] (ICC, Mouse) ICC Mouse
MS Lee, J Jeon, S Park, J Lim, HS Yang Rationally designed bioactive milk-derived protein scaffolds enhanced new bone formation Oncogene, 2022-06-16;20(0):368-380. 2022-06-16 [PMID: 35784638] (IHC, Mouse) IHC Mouse
Yafei Yuan, Wei Wang, Yue Zhang, Qiaohui Hong, Wenhui Huang, Lijuan Li et al. Apelin-13 Attenuates Lipopolysaccharide-Induced Inflammatory Responses and Acute Lung Injury by Regulating PFKFB3-Driven Glycolysis Induced by NOX4-Dependent ROS Journal of Inflammation Research 3/30/2022 [PMID: 35386222]
L Zhang, J Chen, L Yan, Q He, H Xie, M Chen Resveratrol Ameliorates Cardiac Remodeling in a Murine Model of Heart Failure With Preserved Ejection Fraction Frontiers in Pharmacology, 2021-06-10;12(0):646240. 2021-06-10 [PMID: 34177571] (IHC, Mouse) IHC Mouse
Yuan Wang, Shan Zhu, Wen Wei, Yi Tu, Chuang Chen, Junlong Song et al. Interleukin-6 knockout reverses macrophage differentiation imbalance and alleviates cardiac dysfunction in aging mice Aging (Albany NY) 10/25/2020 [PMID: 33099539]
J Ye, Y Wang, Y Xu, Z Wang, L Liu, M Wang, D Ye, J Zhang, Z Yang, Y Lin, Q Ji, J Wan Interleukin-22 deficiency alleviates doxorubicin-induced oxidative stress and cardiac injury via the p38 MAPK/macrophage/Fizz3 axis in mice Redox Biol, 2020-07-07;36(0):101636. 2020-07-07 [PMID: 32863209] (IHC, Mouse) IHC Mouse
A Abbadi, J Loftis, A Wang, M Yu, Y Wang, S Shakya, X Li, EV Maytin, VC Hascall Heparin inhibits pro-inflammatory and promotes anti-inflammatory macrophage polarization under hyperglycemic stress J. Biol. Chem., 2020-02-27;0(0):. 2020-02-27 [PMID: 32107314] (ICC, Mouse) ICC Mouse
Jinjin Peng, Jiacheng Li, Jing Huang, Pan Xu, Heming Huang, Yanjun Liu et al. p300/CBP inhibitor A-485 alleviates acute liver injury by regulating macrophage activation and polarization Theranostics 10/22/2019 [PMID: 31754401]
Show All 22 Publications.

Review for F4/80 Antibody (MAB5580) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using MAB5580:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen F4/80 MAB5580
Enlarge
5
reviewed by:
Verified Customer
IHC-Fr Mouse 06/05/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedLiver
SpeciesMouse
LotMAB5580

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for F4/80 Antibody (MAB5580). (Showing 1 - 5 of 5 FAQs).

  1. Are the F4/80/EMR1 antibodies validated in Simple Western?
    • No, F4/80/EMR1 antibodies are not tested in Simple Western at this moment.
  2. Does F4/80/EMR1 antibodies come in lyophilized form?
    • Yes, we carry 2 F4/80 antibodies in lyophilized form: MAB5580 and NBP1-06975
  3. What is the theoretical molecular weight for F4/80/EMR1 antibodies?
    • The TMW of F4/80/EMR1 antibodies is approximately 160 kDa.
  4. Does this F4/80/EMR1 antibody come in lyophilized form?
    • Yes, we carry this F4/80 antibody in lyophilized form.
  5. What is the immunogen sequence of this F4/80/EMR1 antibodies antibody?
    • A sequence corresponding to Gln28 - Gly931 of mouse F4/80/EMR1.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
1
4
0
3
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2
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1
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Verified Customer
06/05/2018
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol EMR1
Uniprot