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F4/80 Antibody (521204) [Allophycocyanin]

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RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse F4/80/EMR1 APC‑conjugated Monoclonal Antibody (Catalog # FAB5580A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse F4/80/EMR1 APC‑conjugated Monoclonal Antibody (Catalog # FAB5580A, filled histogram) or isotype control antibody (Catalog # IC006A, ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
521204
Clonality
Monoclonal
Host
Rat
Conjugate
Allophycocyanin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB5580U-100UG
Alexa Fluor 405
FAB5580V-100UG
Alexa Fluor 488
FAB5580G-100UG
Alexa Fluor 532
FAB5580X
Alexa Fluor 594
FAB5580T-100UG
Alexa Fluor 647
FAB5580R-100UG
Alexa Fluor 700
FAB5580N-100UG
Alexa Fluor 750
FAB5580S-100UG
Allophycocyanin/Cy7
FAB5580APCCY7
Biotin
FAB5580B
DyLight 350
FAB5580D
DyLight 405
FAB5580E
DyLight 488
FAB5580K
DyLight 550
FAB5580L
DyLight 594
FAB5580M
DyLight 650
FAB5580W
DyLight 680
FAB5580Y
DyLight 755
FAB5580Z
FITC
FAB5580F
HRP
FAB5580H
Janelia Fluor 549
FAB5580I
Janelia Fluor 646
FAB5580J
PE/Atto594
FAB5580PEATT594
PE/Cy5.5
FAB5580PECY55
PE/Cy7
FAB5580PECY7
Phycoerythrin
FAB5580P-100UG
Unconjugated
MAB5580
mFluor Violet 450 SE
FAB5580MFV450
mFluor Violet 500 SE
FAB5580MFV500
mFluor Violet 610 SE
FAB5580MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB5580
Carrier Free
MAB5580
Carrier Free
MAB5580-SP
Carrier Free
FAB5580V-100UG
Carrier Free
FAB5580U-100UG
Carrier Free
FAB5580T-100UG
Carrier Free
FAB5580S-100UG
Carrier Free
FAB5580R-100UG
Carrier Free
FAB5580N-100UG
Carrier Free
FAB5580G-100UG
Carrier Free
FAB5580P-100UG
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F4/80 Antibody (521204) [Allophycocyanin] Summary

Specificity
Detects mouse F4/80/EMR1.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
EMR1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Publications
Read Publications using
FAB5580A in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for F4/80 Antibody (521204) [Allophycocyanin]

  • ADGRE1
  • cell surface glycoprotein F4/80
  • DD7A5-
  • DD7A5-7
  • EGF-like module containing, mucin-like, hormone receptor-like sequence 1
  • EGF-like module receptor 1
  • EGF-like module-containing mucin-like hormone receptor-like 1
  • EGF-TM7
  • Emr
  • EMR1 hormone receptor
  • Emr1
  • F4/80
  • Gpf480
  • Ly7
  • Ly71
  • lymphocyte antigen 71
  • Mouse EMR1
  • Rat EMR1
  • TM7L
  • TM7LN3

Background

F4/80, also known as EMR1, is a 160 kDa extensively glycosylated protein in the EGF-TM7 family of adhesion molecules. It is primarily expressed on macrophages and dendritic cells in mouse but is restricted to eosinophils in human. F4/80 plays a role in the development of peripheral tolerance.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for F4/80 Antibody (FAB5580A)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: Flow Cytometry, IHC.


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Flow Cytometry
(1)
IHC
(1)
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(2)
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Product General Protocols

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FAQs for F4/80 Antibody (FAB5580A). (Showing 1 - 4 of 4 FAQs).

  1. Are the F4/80/EMR1 antibodies validated in Simple Western?
    • No, F4/80/EMR1 antibodies are not tested in Simple Western at this moment.
  2. Does F4/80/EMR1 antibodies come in lyophilized form?
    • Yes, we carry 2 F4/80 antibodies in lyophilized form: MAB5580 and NBP1-06975
  3. What is the theoretical molecular weight for F4/80/EMR1 antibodies?
    • The TMW of F4/80/EMR1 antibodies is approximately 160 kDa.
  4. What is the immunogen sequence of this F4/80/EMR1 antibodies antibody?
    • A sequence corresponding to Gln28 - Gly931 of mouse F4/80/EMR1.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol EMR1
Uniprot