Ezrin Antibody (3C12)

Images

 
Immunocytochemistry/ Immunofluorescence: Ezrin Antibody (3C12) [NB600-1310] - Cultured Rat hippocampal neurons, courtesy of customer.
Immunohistochemistry-Paraffin: Ezrin Antibody (3C12) [NB600-1310] - Formalin fixed paraffin embedded lung adenocarcinoma stained with Ezrin antibody (NB600-1310).
Immunohistochemistry-Paraffin: Ezrin Antibody (3C12) [NB600-1310] - Formalin fixed paraffin embedded human lung stained with Ezrin antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
3C12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Ezrin Antibody (3C12) Summary

Immunogen
BALB/C mice were immunized with a recombinant fragment of human ezrin corresponding to amino acid 362-585.
Localization
Cell membrane
Specificity
This antibody reacts with a protein of 81 kDa. Ezrin serves as a tyrosine kinase substrate and is phosphorylated in EGF-stimulated cells. Ezrin is a cytoplasmic protein, enriched in microvilli and other cell surfaces. Ezrin has an actin-binding capacity. Ezrin is expressed in epithelial cells but not in mesenchymal cells. Ezrin is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EZR
Purity
Ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:100-1:750
Application Notes
IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.
Control
Ezrin Overexpression Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB600-1310 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
Ascites
Preservative
0.05% Sodium Azide
Purity
Ascites

Alternate Names for Ezrin Antibody (3C12)

  • CVIL
  • CVL
  • Cytovillin 2
  • Cytovillin
  • DKFZp762H157
  • EZR
  • Ezrin
  • FLJ26216
  • p81
  • VIL2
  • VIL2MGC1584
  • villin 2 (ezrin)
  • Villin 2
  • villin-2

Background

Ezrin serves as a tyrosine kinase substrate and is phosphorylated in EGF-stimulated cells. It is a cytoplasmic protein enriched in microvilli and other cell surfaces. Ezrin has an actin-binding capacity and is expressed in epithelial cells but not in mesenchymal cells. It is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ezrin Antibody (NB600-1310) (0)

There are no publications for Ezrin Antibody (NB600-1310).
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Review for Ezrin Antibody (NB600-1310) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB600-1310:
Filter by Applications
ICC
(1)
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(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Ezrin NB600-1310
Enlarge
4
reviewed by:
Janosch Heller
ICC Rat 03/27/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedCultured rat hippocampal neurons
SpeciesRat
LotN/A

Comments

CommentsFixation: 3% formaldehyde (TAAB) in PBS, 15 minutes RT
Blocking: 10% donkey serum in PBS, 1 hour RT
Permeabilization: 0.2% Triton-X100
Primary Antibody: 1:100 in 5% horse serum in PBS with 0.2% Triton, overnight, 4°C
Wash: three times wash with PBS with 0.2% Triton, 10 min each
Secondary Antibody: Invitrogen goat anti-mouse Alexa568, 1:500 in PBS with 0.2% Triton, 2 hours, RT
Wash: three times wash with PBS, 10 min each
Microscope: Confocal (Zeiss LSM710)

The antibody labels actin filaments in filopodia.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ezrin Antibody (NB600-1310) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Ezrin Products

Research Areas for Ezrin Antibody (NB600-1310)

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Blogs on Ezrin

There are no specific blogs for Ezrin, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Janosch Heller
03/27/2017
Application: ICC
Species: Rat

Bioinformatics

Gene Symbol EZR
Entrez