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ERG Antibody (OTI4H7) - Azide and BSA Free

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Western Blot: ERG Antibody (OTI4H7) - Azide and BSA Free [NBP2-70686] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERG.
Immunocytochemistry/ Immunofluorescence: ERG Antibody (OTI4H7) - Azide and BSA Free [NBP2-70686] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY ERG.
Immunohistochemistry: ERG Antibody (OTI4H7) - Azide and BSA Free [NBP2-70686] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: ERG Antibody (OTI4H7) - Azide and BSA Free [NBP2-70686] - Analysis of extracts (10ug) from 8 Human tissue (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
Immunohistochemistry: ERG Antibody (OTI4H7) - Azide and BSA Free [NBP2-70686] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: ERG Antibody (OTI4H7) - Azide and BSA Free [NBP2-70686] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI4H7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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ERG Antibody (OTI4H7) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human ERG(NP_891548) produced in HEk293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ERG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
53.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for ERG Antibody (OTI4H7) - Azide and BSA Free

  • transcriptional regulator ERG
  • 10ets-related
  • Erg
  • Erg-3
  • p55
  • TMPRSS2-ERG prostate cancer specific
  • transcriptional regulator ERG
  • Transforming protein ERG
  • v-ets erythroblastosis virus E26 oncogene homolog (avian)
  • v-ets erythroblastosis virus E26 oncogene like (avian)
  • v-ets erythroblastosis virus E26 oncogene like

Background

"ERG is a transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ERG Antibody (NBP2-70686). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for an antibody that can IP Erg protein and doesn't cross react with Fli protein.
    • I have spoken to the lab and they have informed me that actually that the antibody (catalog #NBP1-95857) is confirmed not to cross react with Fli1 protein so it should be a viable choice for you in your experiments on Erg.

Secondary Antibodies

 

Isotype Controls

Additional ERG Products

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Bioinformatics

Gene Symbol ERG