![Western Blot: Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free [NBP2-80718] - Total protein from mouse placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocking in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-CD105 (NB100-77666) in 1% milk/TBST and detected with an anti-rat HRP conjugated secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MJ7-18-Azide-and-BSA-Free-Western-Blot-NBP2-80718-img0001.jpg "Western Blot: Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free [NBP2-80718] - Total protein from mouse placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocking in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-CD105 (NB100-77666) in 1% milk/TBST and detected with an anti-rat HRP conjugated secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
| Reactivity | MuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | MJ7/18 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Inflamed mouse skin |
| Epitope | Endothelial cell selective TGB1 binding molecule-endoglin |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | ENG |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Tris-Glycine |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | ENG |
![Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free [NBP2-80718] - Using the DyLight 550 direct conjugate Staining of CD105 (endoglin) in 4T1 tumors after radioimmunotherapy shows CD105 expression (red), blood vessels (green), and nuclei (blue).](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MJ7-18-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80718-img0002.jpg "Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free [NBP2-80718] - Using the DyLight 550 direct conjugate Staining of CD105 (endoglin) in 4T1 tumors after radioimmunotherapy shows CD105 expression (red), blood vessels (green), and nuclei (blue).")
![Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free [NBP2-80718] - Analysis of a FFPE mouse ductus deference using a 1:50 dilution of CD105 antibody (clone MJ7/18) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MJ7-18-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80718-img0003.jpg "Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free [NBP2-80718] - Analysis of a FFPE mouse ductus deference using a 1:50 dilution of CD105 antibody (clone MJ7/18) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)")
![Flow (Cell Surface): Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free [NBP2-80718] - A surface stain was performed on MS-1 Cells with Endoglin/CD105 (MJ7/18) antibody NB100-77666 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by rat F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0113, R&D Systems). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MJ7-18-Azide-and-BSA-Free-Flow-Cell-Surface-NBP2-80718-img0004.jpg "Flow (Cell Surface): Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free [NBP2-80718] - A surface stain was performed on MS-1 Cells with Endoglin/CD105 (MJ7/18) antibody NB100-77666 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by rat F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0113, R&D Systems). Image from the standard format of this antibody.")
![Flow Cytometry: Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free [NBP2-80718] - Analysis of Biotin conjugate of NB100-77666. Surface staining of bEND.3 cell line with 0.25 ug of Anti-Mouse CD105 (Endoglin) Biotin followed by Streptavidin PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MJ7-18-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80718-img0005.jpg "Flow Cytometry: Endoglin/CD105 Antibody (MJ7/18) - Azide and BSA Free [NBP2-80718] - Analysis of Biotin conjugate of NB100-77666. Surface staining of bEND.3 cell line with 0.25 ug of Anti-Mouse CD105 (Endoglin) Biotin followed by Streptavidin PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.")
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![Immunohistochemistry-Frozen: Endoglin/CD105 Antibody (MJ7/18) [DyLight 550] [NB100-77666R] - Staining of CD105 (endoglin) in 4T1 tumors after radioimmunotherapy shows CD105 expression (red), blood vessels (green), and nuclei (blue).](https://images.novusbio.com/images/Endoglin-CD105-Antibody-MJ7-18-DyLight-550-Immunohistochemistry-Frozen-NB100-77666R-img0001.jpg "Immunohistochemistry-Frozen: Endoglin/CD105 Antibody (MJ7/18) [DyLight 550] [NB100-77666R] - Staining of CD105 (endoglin) in 4T1 tumors after radioimmunotherapy shows CD105 expression (red), blood vessels (green), and nuclei (blue).")