![Western Blot: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Lane A: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:42 kDa Observed band size:54 kDa](http://images.novusbio.com/fullsize/EMMPRIN-CD147-Antibody-125-Western-Blot-NBP2-89320-img0011.jpg "Western Blot: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Lane A: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:42 kDa Observed band size:54 kDa")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, IP |
| Clone | 125 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | This antibody was obtained from a rabbit immunized with purified, recombinant Human EMMPRIN/CD147 (Accession#: NP_940991.1; Met 1-His 205). |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | BSG |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | BSG |
![Immunocytochemistry/Immunofluorescence: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Staining of CD147 in monkey stomach with rabbit monoclonal antibody (1:1000, frozen section). The image showing membrane staining of gastric gland cell. The right panel: merge with DAPI](http://images.novusbio.com/fullsize/EMMPRIN-CD147-Antibody-125-Immunocytochemistry-Immunofluorescence-NBP2-89320-img0002.jpg "Immunocytochemistry/Immunofluorescence: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Staining of CD147 in monkey stomach with rabbit monoclonal antibody (1:1000, frozen section). The image showing membrane staining of gastric gland cell. The right panel: merge with DAPI")
![Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Staining of human CD147 in human esophagus with rabbit monoclonal antibody (1:5000). The image showing membrane staining of squamous epithelium cell. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).](http://images.novusbio.com/fullsize/EMMPRIN-CD147-Antibody-125-Immunohistochemistry-Paraffin-NBP2-89320-img0008.jpg "Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Staining of human CD147 in human esophagus with rabbit monoclonal antibody (1:5000). The image showing membrane staining of squamous epithelium cell. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).")
![Flow Cytometry: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Analysis of anti-CD147 reactivity on HeLa cells.](http://images.novusbio.com/fullsize/EMMPRIN-CD147-Antibody-125-Flow-Cytometry-NBP2-89320-img0009.jpg "Flow Cytometry: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Analysis of anti-CD147 reactivity on HeLa cells.")
![Immunocytochemistry/Immunofluorescence: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Staining of human CD147 in HeLa cells with rabbit monoclonal antibody (1:200). The image showing membrane staining of HeLa cells.](http://images.novusbio.com/fullsize/EMMPRIN-CD147-Antibody-125-Immunocytochemistry-Immunofluorescence-NBP2-89320-img0001.jpg "Immunocytochemistry/Immunofluorescence: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Staining of human CD147 in HeLa cells with rabbit monoclonal antibody (1:200). The image showing membrane staining of HeLa cells.")
![Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Staining of human CD147 in human stomach with rabbit monoclonal antibody (1:5000). The image showing membrane staining of epithelium cell.](http://images.novusbio.com/fullsize/EMMPRIN-CD147-Antibody-125-Immunohistochemistry-Paraffin-NBP2-89320-img0003.jpg "Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Staining of human CD147 in human stomach with rabbit monoclonal antibody (1:5000). The image showing membrane staining of epithelium cell.")
![Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Staining of human CD147 in human rectal cancer with rabbit monoclonal antibody (1:5000). The image showing membrane staining of epithelium cell in intestinal gland.](http://images.novusbio.com/fullsize/EMMPRIN-CD147-Antibody-125-Immunohistochemistry-Paraffin-NBP2-89320-img0004.jpg "Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Staining of human CD147 in human rectal cancer with rabbit monoclonal antibody (1:5000). The image showing membrane staining of epithelium cell in intestinal gland.")
![Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Staining of human CD147 in human placenta with rabbit monoclonal antibody (1:5000). The image showing positive staining of trophoblast.](http://images.novusbio.com/fullsize/EMMPRIN-CD147-Antibody-125-Immunohistochemistry-Paraffin-NBP2-89320-img0005.jpg "Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Staining of human CD147 in human placenta with rabbit monoclonal antibody (1:5000). The image showing positive staining of trophoblast.")
![Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Staining of human CD147 in human liver with rabbit monoclonal antibody (1:5000). The image showing membrane staining of hepatocyte.](http://images.novusbio.com/fullsize/EMMPRIN-CD147-Antibody-125-Immunohistochemistry-Paraffin-NBP2-89320-img0006.jpg "Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Staining of human CD147 in human liver with rabbit monoclonal antibody (1:5000). The image showing membrane staining of hepatocyte.")
![Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Staining of human CD147 in human gastric cancer with rabbit monoclonal antibody (1:5000). The image showing membrane staining of epithelium cell.](http://images.novusbio.com/fullsize/EMMPRIN-CD147-Antibody-125-Immunohistochemistry-Paraffin-NBP2-89320-img0007.jpg "Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Staining of human CD147 in human gastric cancer with rabbit monoclonal antibody (1:5000). The image showing membrane staining of epithelium cell.")
![Immunoprecipitation: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Lane A:0.5 mg K562 Whole Cell Lysate 2 uL anti-CD147 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CD147 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 42 kDaObserved band size: 50 kDa](http://images.novusbio.com/fullsize/EMMPRIN-CD147-Antibody-125-Immunoprecipitation-NBP2-89320-img0010.jpg "Immunoprecipitation: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Lane A:0.5 mg K562 Whole Cell Lysate 2 uL anti-CD147 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CD147 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 42 kDaObserved band size: 50 kDa")
![Knockout Validated: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - NBP2-89320 at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: CD147 knockout Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:42 kDa. Observed band size:35-55 kDa](http://images.novusbio.com/fullsize/nbp2-89320_rabbit-monoclonal-emmprin-cd147-antibody-125-136202422345057.png "Knockout Validated: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - NBP2-89320 at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: CD147 knockout Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:42 kDa. Observed band size:35-55 kDa")
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![N/A MMP-2 [HRP]](https://images.novusbio.com/images2/DATA_MMP2_MMP200_ELISA_1003.jpg)
![N/A MMP-2 [HRP]](https://images.novusbio.com/images2/MMP2_MMP200_ELISA_474.jpg)
![N/A MMP-9 [HRP]](https://images.novusbio.com/images2/MMP-9_DMP900_ELISA_148.jpg)
![N/A MMP-9 [HRP]](https://images.novusbio.com/images2/DATA_MMP9_DMP900_ELISA_770.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_871.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg)
![Knockout Validated MMP-1 Antibody (36665) [Unconjugated]](https://images.novusbio.com/images2/MMP1_MAB901_Knockout_Validated_21762.jpg)
![Immunohistochemistry MMP-1 Antibody (36665) [Unconjugated]](https://images.novusbio.com/images2/MMP-1_MAB901_Immunohistochemistry_7049.jpg)
![Immunohistochemistry MMP-1 Antibody (36665) [Unconjugated]](https://images.novusbio.com/images2/MMP-1_MAB901_Immunohistochemistry_9677.jpg)
![Flow Cytometry MMP-14/MT1-MMP Antibody (128527) [Unconjugated]](https://images.novusbio.com/images2/MMP-14_MAB9181_Flow_Cytometry_11258.jpg)
![Immunohistochemistry MMP-14/MT1-MMP Antibody (128527) [Unconjugated]](https://images.novusbio.com/images2/mab9181_human-mmp-14-mt1-mmp-mab-clone-128527-immunohistochemistry-239202215152.jpg)
![Western Blot ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg)
![Immunohistochemistry ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg)
![Knockout Validated ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg)
![Western Blot Chymase/CMA1/Mast Cell Chymase Antibody (422818) [Unconjugated]](https://images.novusbio.com/images2/Chymase_MAB4099_Western_Blot_21236.jpg)
![ELISA Chymase/CMA1/Mast Cell Chymase Antibody (422818) [Unconjugated]](https://images.novusbio.com/images2/mab4099_human-chymase-cma1-mab-clone-422818-elisa-6122021135313.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")