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MMP-14/MT1-MMP Antibody (128527) [Unconjugated]

 (11 Publications)
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MDA‑MB‑231 human breast cancer cell line was stained with Mouse Anti-Human MMP‑14/MT1‑MMP Monoclonal Antibody (Catalog # MAB9181, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
MDA‑MB‑231 human breast cancer cell line was stained with Mouse Anti-Human MMP‑14/MT1‑MMP Monoclonal Antibody (Catalog # MAB9181, filled histogram) or isotype control antibody (Catalog # MAB0041, open ...read more
MMP‑14/MT1‑MMP was detected in immersion fixed paraffin-embedded sections of Human Prostate Cancer using Mouse Anti-Human MMP‑14/MT1‑MMP Monoclonal Antibody (Catalog # MAB9181) at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IP, CyTOF-ready
Clone
128527
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB9181U-100UG
Alexa Fluor 405
FAB9181V-100UG
Alexa Fluor 488
FAB9181G
Alexa Fluor 594
FAB9181T-100UG
Alexa Fluor 647
FAB9181R-100UG
Alexa Fluor 700
FAB9181N-100UG
Alexa Fluor 750
FAB9181S-100UG
Allophycocyanin
FAB9181A
Phycoerythrin
FAB9181P
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB9181A
Carrier Free
FAB9181G
Carrier Free
FAB9181P
Carrier Free
MAB9181-SP
Carrier Free
MAB9181-500
Carrier Free
FAB9181V-100UG
Carrier Free
FAB9181U-100UG
Carrier Free
FAB9181T-100UG
Carrier Free
FAB9181S-100UG
Carrier Free
FAB9181R-100UG
Carrier Free
FAB9181N-100UG
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MMP-14/MT1-MMP Antibody (128527) [Unconjugated] Summary

Specificity
Detects human MMP‑14/MT1‑MMP in direct ELISAs and Western blots. Does not detect E. coli-expressed recombinant human MMP-14 catalytic domain (aa 112-284).
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MMP14
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB9181 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-14/MT1-MMP Antibody (128527) [Unconjugated]

  • EC 3.4.24
  • EC 3.4.24.80
  • matrix metallopeptidase 14 (membrane-inserted)
  • matrix metalloproteinase 14 (membrane-inserted)
  • matrix metalloproteinase-14
  • membrane type 1 metalloprotease
  • Membrane-type matrix metalloproteinase 1
  • Membrane-type-1 matrix metalloproteinase
  • MMP14
  • MMP-14
  • MMP-X1
  • MT1MMP
  • MT1-MMP
  • MT1-MMPMTMMP1
  • MT-MMP 1
  • MT-MMP1

Background

As the first member of membrane type (MT) MMPs, MMP-14, also known as MT1-MMP, plays an important role in extracellular matrix (ECM) remodeling by being able to degrade type I collagen, activate pro-MMP-2 and process cell adhesion molecules such as CD44 and integrin alpha V (1). MMP-14 is therefore a key enzyme in many physiological and pathological processes such as angiogenesis and tumor invasion. Structurally, MMP-14 consists of the following domains: a pro domain containing the furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplasmic tail (2).

  1. Seike, M. (2003) Cancer Lett. 194:1.
  2. Sato, H. et al. (1994) Nature 370:61.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMP-14/MT1-MMP Antibody (MAB9181)(11)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 6 applications: CyTof, Flow Cytometry, ICC, IHC, IHC-Fr, Western Blot.


Filter By Application
CyTof
(2)
Flow Cytometry
(3)
ICC
(1)
IHC
(1)
IHC-Fr
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(8)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using MAB9181 Applications Species
Gerwinn T, Salemi S, Schori LJ et al. Improved contractile potential in detrusor microtissues from pediatric patients with end stage lower urinary tract dysfunction Frontiers in Cell and Developmental Biology 2022-10-04 [PMID: 36268506]
S Parveen, A Khamari, J Raju, MG Coppolino, S Datta Syntaxin 7 contributes to breast cancer cell invasion by promoting invadopodia formation Journal of Cell Science, 2022-06-28;135(12):. 2022-06-28 [PMID: 35762511] (ICC, Human) ICC Human
TB Layton, L Williams, N Yang, M Zhang, C Lee, M Feldmann, G Trujillo, D Furniss, J Nanchahal A vasculature niche orchestrates stromal cell phenotype through PDGF signaling: Importance in human fibrotic disease Proceedings of the National Academy of Sciences of the United States of America, 2022-03-23;119(13):e2120336119. 2022-03-23 [PMID: 35320046] (CyTof, IHC, Human) CyTof, IHC Human
Keizo Takenaga, Takahiro Ochiya, Hideya Endo Inhibition of the invasion and metastasis of mammary carcinoma cells by NBD peptide targeting S100A4 via the suppression of the Sp1/MMP‑14 axis International Journal of Oncology 1/21/2021 [PMID: 33650647]
TB Layton, L Williams, F McCann, M Zhang, M Fritzsche, H Colin-York, M Cabrita, MTH Ng, M Feldmann, SN Sansom, D Furniss, W Xie, J Nanchahal Cellular census of human fibrosis defines functionally distinct stromal cell types and states Nat Commun, 2020-06-02;11(1):2768. 2020-06-02 [PMID: 32488016] (CyTof, Human) CyTof Human
Localization, Shedding, Regulation and Function of Aminopeptidase N/CD13 on Fibroblast like Synoviocytes PLoS ONE, 2016-09-22;11(9):e0162008. 2016-09-22 [PMID: 27658265] (IHC-Fr, Human) IHC-Fr Human
Hiroaki Kajiho RAB2A controls MT1-MMP endocytic and E-cadherin polarized Golgi trafficking to promote invasive breast cancer programs EMBO Rep, 2016-06-02;0(0):. 6/2/2016 [PMID: 27255086]
Kaimal R, Aljumaily R, Tressel S, Pradhan R, Covic L, Kuliopulos A, Zarwan C, Kim Y, Sharifi S, Agarwal A Selective blockade of matrix metalloprotease-14 with a monoclonal antibody abrogates invasion, angiogenesis, and tumor growth in ovarian cancer. Cancer Res, 2013-02-19;73(8):2457-67. 2013-02-19 [PMID: 23423981] (Flow Cytometry, Human) Flow Cytometry Human
Mierke CT, Bretz N, Altevogt P Contractile forces contribute to increased glycosylphosphatidylinositol-anchored receptor CD24-facilitated cancer cell invasion. J. Biol. Chem., 2011-08-02;286(40):34858-71. 2011-08-02 [PMID: 21828044] (Flow Cytometry, Human) Flow Cytometry Human
Mierke CT, Frey B, Fellner M, Herrmann M, Fabry B Integrin alpha5beta1 facilitates cancer cell invasion through enhanced contractile forces. J. Cell. Sci., 2011-01-11;124(0):369-83. 2011-01-11 [PMID: 21224397] (Flow Cytometry, Human) Flow Cytometry Human
Show All 11 Publications.

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Bioinformatics

Gene Symbol MMP14
Uniprot