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EMMPRIN/CD147 Antibodies

We offer EMMPRIN/CD147 Antibodies for use in common research applications: ELISA, Flow Cytometry, Functional, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot. Each EMMPRIN/CD147 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our EMMPRIN/CD147 Antibodies can be used in a variety of model species: Canine, Equine, Human, Mouse, Porcine, Primate, Rat. Use the list below to choose the EMMPRIN/CD147 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our EMMPRIN/CD147 polyclonal antibodies and browse our EMMPRIN/CD147 monoclonal antibody catalog.

Alternate Names for EMMPRIN/CD147 Antibodies

anti-EMMPRIN/CD147 antibody, anti-BSG antibody, anti-5F7 antibody, anti-basigin (Ok blood group) antibody, anti-basigin antibody, anti-CD147 antibody, anti-CD147 antigen antibody, anti-Collagenase stimulatory factor antibody, anti-EMMPRINTCSF antibody, anti-Extracellular matrix metalloproteinase inducer antibody, anti-Leukocyte activation antigen M6 antibody, anti-M6 antibody, anti-OK antibody, anti-OK blood group antigen antibody, anti-Tumor cell-derived collagenase stimulatory factor antibody

33 Results for "emmprin-cd147" in Primary Antibodies

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AF972

EMMPRIN/CD147 Antibody [Unconjugated]

(1 Review)

(15 Publications)

100 μg

AF972

Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for EMMPRIN/CD147 at approximately 38-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

EMMPRIN/CD147 was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using Goat Anti-Human EMMPRIN/ CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

EMMPRIN/CD147 was detected in immersion fixed paraffin-embedded sections of human cervix using Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for EMMPRIN/CD147 at approximately 70 kDa (as indicated) using 10 µg/mL of Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

<b>Genetic Strategies Validation. </b>Western blot shows lysates of HEK293T human embryonic kidney parental cell line and EMMPRIN/CD147 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EMMPRIN/CD147 at approximately 51 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Recombinant Human EMMPRIN/CD147 protein was serially diluted 2-fold and captured by Mouse Anti-Human EMMPRIN/CD147 Monoclonal Antibody (MAB972) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF972

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF972-SP

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NB100-64848

EMMPRIN/CD147 Antibody (OX-47) - BSA Free

(6 Publications)

0.1 ml

NB100-64848

Reactivity:

Species Glossary

Reactivity: Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-64848AF350
Alexa Fluor 405	NB100-64848AF405
Alexa Fluor 488	NB100-64848AF488
Alexa Fluor 532	NB100-64848AF532
Alexa Fluor 594	NB100-64848AF594
Alexa Fluor 647	NB100-64848AF647
Alexa Fluor 700	NB100-64848AF700
Alexa Fluor 750	NB100-64848AF750
Allophycocyanin	NB100-64848APC
Biotin	NB100-64848B
CoraFluor 1	NB100-64848CL1
DyLight 350	NB100-64848UV
DyLight 405	NB100-64848V
DyLight 488	NB100-64848G
DyLight 550	NB100-64848R
DyLight 594	NB100-64848DL594
DyLight 650	NB100-64848C
DyLight 680	NB100-64848FR
DyLight 755	NB100-64848IR
FITC	NB100-64848F
HRP	NB100-64848H
Janelia Fluor 525	NB100-64848JF525
Janelia Fluor 549	NB100-64848JF549
Janelia Fluor 646	NB100-64848JF646
Janelia Fluor 669	NB100-64848JF669
PE	NB100-64848PE
PerCP	NB100-64848PCP
mFluor Violet 450 SE	NB100-64848MFV450
mFluor Violet 500 SE	NB100-64848MFV500
mFluor Violet 610 SE	NB100-64848MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-64848SS
Azide and BSA Free	NBP2-80717

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MAB972

EMMPRIN/CD147 Antibody (109403) [Unconjugated]

(10 Publications)

100 μg

MAB972

Western blot shows lysates of human brain (cerebellum) tissue, human heart tissue, and human liver tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB972) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for EMMPRIN/CD147 at approximately 40-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Recombinant Human EMMPRIN/CD147 protein was serially diluted 2-fold and captured by Mouse Anti-Human EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB972) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (AF972) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Silencing CD147 inhibits HCMV entry into endothelial cells. (A) Lysates from a control endothelial cell line (tAECs) transduced with an empty lentivirus vector (Ctl) or an endothelial cell line (tAECs) transduced with a lentivirus expressing a CD147-specific shRNA (CD147 shRNA; clone ID V3LHS_412785; Dharmacon) were analyzed in Western blot assays to determine the level of CD147 silencing. Membranes were probed with either anti-CD147 MAb 109403 or a rabbit polyclonal antibody against beta-actin (a loading control). (B) Human endothelial cells (tAECs) were transduced with an empty lentivirus vector (Ctl, lacking any shRNA) or a lentivirus vector expressing an shRNA to CD147 (CD147 shRNA) and then selected by puromycin. The cells lines were then infected with HCMV strain BADrUL131, and the level of entry was assessed by monitoring GFP expression from at least three independent wells. (C) The lentivirus-transduced cell lines in panel B were infected with HSV-1 VP26-GFP and analyzed for GFP expression after 24 h. (D) Lysates from control fibroblasts transduced with an empty lentivirus vectors (Ctl) or CD147 shRNA-expressing fibroblasts (CD147 shRNA) were analyzed by Western blotting to characterize CD147 silencing as described for panel A. (E) Fibroblast cell lines that had been transduced with an empty lentivirus vector (Ctl) or a lentivirus vector expressing an shRNA to CD147 (CD147 shRNA) were established using puromycin and then infected with HCMV BADrUL131, after which the number of GFP-positive cells was analyzed. Image collected and cropped by CiteAb from the following publication (//journals.asm.org/doi/10.1128/mBio.00781-18), licensed under a CC-BY license. Not internally tested by R&D Systems.

Clone 10 binding efficiency in human cells and tissue.(A) Using fluorescence-activated cell sorting (FACS) analysis, human fetal astrocytes stained for glial fibrillary acidic protein (GFAP) were additionally stained with hybridoma supernatants from several clones (1 to 13, no clone 5) and with the commercial anti-human extracellular matrix metalloproteinase inducer (EMMPRIN) antibody (R&D Systems). (B) Immunofluorescence staining of postmortem multiple sclerosis (MS) brain samples for laminin/CD45 (1; higher magnification inset) and GFAP (3, 4, 6, 7, 9, 10), and with clone 10 (2, 4), IgM isotype control (5, 7) and commercial anti-EMMPRIN (US Biological, Table 1) antibodies (8, 10). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22480370), licensed under a CC-BY license. Not internally tested by R&D Systems.

Clone 10 binds most efficiently to extracellular matrix metalloproteinase inducer (EMMPRIN) in western blots. Hybridoma supernatants from several clones are used in a western blot to detect 5 μg of recombinant human EMMPRIN (rhE; lane 1), 10 μg of mouse central nervous system (CNS) homogenate (m; lane 2) and 10 μg of human CNS homogenate (h; lane 3), EMMPRIN has an approximate molecular weight of 55 kDa. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22480370), licensed under a CC-BY license. Not internally tested by R&D Systems.

Inhibition of HCMV entry via anti-CD147 antibodies. (A) Human ARPE-19 epithelial cells were left untreated (No Ab) or were pretreated with MAbs specific for the transferrin receptor (TfR) or CD147 (MAbs 9B10, M61, 109403, 2F5, and 12G10) for 1 h at 37°C and then HCMV BADrUL131 was added to these cells in the presence of the MAbs for an additional 2 h at 37°C. The virus inoculum was removed, and the culture medium was replenished with fresh growth medium that contained the MAbs for 24 h, after which HCMV entry was assessed. (B) Human retinal epithelial cells were either left untreated or treated with antibodies as described above and then incubated with HSV-1 F-BAC VP26GFP, an HSV recombinant that express a GFP-tagged tegument protein. (C and D) Antibody inhibition assays were performed as described for panel A with HUVECs or fibroblast cells, respectively, and then challenged with HCMV BADrUL131. Under all experimental conditions, virus entry was quantified by counting GFP-positive from at least three independent wells, and these values compared to the numbers of GFP-positive cells among those not treated with antibodies. (E) Lysates from epithelial, endothelial, or fibroblast cells were analyzed by Western blotting to detect endogenous CD147 by probing membranes with anti-CD147 MAb 109403 or with polyclonal antibodies against beta-actin as a loading control. Image collected and cropped by CiteAb from the following publication (//journals.asm.org/doi/10.1128/mBio.00781-18), licensed under a CC-BY license. Not internally tested by R&D Systems.

HCMV colocalizes with CD147 in early endosomes. ARPE-19 epithelial cells on glass slides were transduced with a baculovirus expressing a fluorescent-tagged early endosome marker, EEA-1. The cells were then incubated with HCMV UL32-GFP virus particles for 1 h at 4°C and then shifted to 37°C for 1 h. The cells were fixed and processed for immunofluorescent staining with an anti-CD147 MAb (109403) followed by a 649-conjugated secondary antibody to detect CD147 and then analyzed by deconvolution microscopy. (A and B) Representative images with arrows depict HCMV particles (green) colocalizing with CD147 (red) in EEA-1-labeled endosomes (blue). (C to E) More highly magnified images of HCMV particles (green) colocalizing with CD147 (red) and EEA-1-labeled endosomes (blue). The scale bars on the bottom left of each panel represent 1 µm. Image collected and cropped by CiteAb from the following publication (//journals.asm.org/doi/10.1128/mBio.00781-18), licensed under a CC-BY license. Not internally tested by R&D Systems.

HCMV colocalizes with CD147 patches on the surface of ARPE-19 cells. (A) ARPE-19 cells on glass slides were incubated with anti-CD147 MAb 109403 and HCMV UL32-GFP virus particles at 4° for 1 h, then shifted to 37° for 15 min, and then fixed. The fixed cells were then incubated with goat anti-mouse–594 fluorescent secondary antibody to detect surface CD147 and then the cells were analyzed by deconvolution microscopy. Arrows indicate green fluorescent HCMV virus particles that overlapped with red fluorescent channel signal from CD147 surface patches. (B and C) Magnified images of the squared areas from panel A. (D) ARPE-19 cells were incubated with anti-EGFR MAb (LA1) and HCMV UL32-GFP virus particles at 4°C for 1 h, then shifted to 37°C for 15 min, fixed, and then processed to detect surface EGFR as described for panel A. The scale bars on the bottom right of each panel represent 1 µm. Image collected and cropped by CiteAb from the following publication (//journals.asm.org/doi/10.1128/mBio.00781-18), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effects of soluble CD147 on HCMV entry and binding to soluble HCMV glycoproteins. (A) A soluble form of CD147 that was fused to a polyhistidine epitope tag and purified from the tissue culture supernatant of plasmid-transfected 293E cells via use of nickel-agarose was analyzed by electrophoresis, polyacrylamide gel staining, and Coomassie brilliant blue stain. (B) Soluble CD147 was bound to nickel-coated plates and then analyzed in an ELISA. Individual wells were incubated with either a control MAb to transferrin (TfR) or CD147-specific MAbs 109403, 12G10, or 2F5, followed by goat anti-mouse–HRP conjugate. Controls also included incubation with secondary antibodies only (secondary). The bound antibodies were detected by chemiluminescence by adding Turbo-TMB ELISA substrate (Thermo Fisher), and absorbance was read using a precision plate reader (Molecular Dynamics). (C) HCMV BADrUL131 virus particles were incubated with soluble CD147 (sol. CD147+) at 100 µg ml−1 or no protein (sol. CD147 -) for 1 h at 37°C and then added to ARPE-19 epithelial cells or HUVECs for 2 h at 37°C. The virus inoclula were removed, and cells were incubated an additional 24 h before the numbers of GFP+ cells were assessed. Relative infectivity was calculated by comparing the numbers of GFP+ cells in soluble CD147 treated groups versus no-protein controls. (D) Soluble CD147 (black bars) or soluble PDGFR alpha (gray bars) were allowed to adsorb onto microtiter plates and then incubated with soluble gH/gL (dimer), trimer, or pentamer complexes. The plates were washed and then incubated with anti-gH MAb 14-4b, washed, and incubated with goat anti-mouse–HRP conjugate. Positive controls included anti-CD147 or anti-PDGFR alpha MAbs followed by secondary goat anti-mouse–HRP conjugate (CD147 pos. and PDGFR alpha pos.). Negative controls involved wells with adsorbed proteins incubated with secondary antibodies only (secondary). Chemiluminescence was detected as described for panel B. Image collected and cropped by CiteAb from the following publication (//journals.asm.org/doi/10.1128/mBio.00781-18), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB972-SP
Carrier Free	MAB972-500

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AF772

EMMPRIN/CD147 Antibody [Unconjugated]

(10 Publications)

100 μg

AF772

Western blot shows lysates of mouse brain tissue, mouse heart tissue, and mouse small intestine tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF772) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EMMPRIN/CD147 at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of mouse brain (cortex) tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for EMMPRIN/CD147 at approximately 62 kDa (as indicated) using 2 µg/mL of Goat Anti-Mouse EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF772) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF772

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF772-SP

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NB500-430

EMMPRIN/CD147 Antibody (MEM-M6/1) - BSA Free

(4 Publications)

0.1 mg

NB500-430

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (MEM-M6/1) [NB500-430] - Staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/1) [NB500-430] - Surface staining of human peripheral blood with anti-human CD147 (MEM-M6/1) biotin.

Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/1) [NB500-430] - Surface staining of human peripheral blood with anti-human CD147 (MEM-M6/1) FITC.

Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/1) [NB500-430] - Staining of human peripheral blood with anti-human CD147 (MEM-M6/1) Alexa Fluor® 488.

Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/1) [NB500-430] - Staining of human peripheral blood with anti-human CD147 (MEM-M6/1) PE.

Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/1) [NB500-430] - Staining of human peripheral blood with anti-human CD147 (MEM-M6/1) APC.

Reactivity:

Hu, Eq

Species Glossary

Reactivity: Hu, Eq

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB500-430AF350
Alexa Fluor 405	NB500-430AF405
Alexa Fluor 488	NB500-430AF488
Alexa Fluor 532	NB500-430AF532
Alexa Fluor 594	NB500-430AF594
Alexa Fluor 647	NB500-430AF647
Alexa Fluor 700	NB500-430AF700
Alexa Fluor 750	NB500-430AF750
Allophycocyanin	NB500-430APC
Biotin	NB500-430B
CoraFluor 1	NB500-430CL1
DyLight 350	NB500-430UV
DyLight 405	NB500-430V
DyLight 488	NB500-430G
DyLight 550	NB500-430R
DyLight 594	NB500-430DL594
DyLight 650	NB500-430C
DyLight 680	NB500-430FR
DyLight 755	NB500-430IR
FITC	NB500-430F
HRP	NB500-430H
Janelia Fluor 525	NB500-430JF525
Janelia Fluor 549	NB500-430JF549
Janelia Fluor 646	NB500-430JF646
Janelia Fluor 669	NB500-430JF669
PE	NB500-430PE
PerCP	NB500-430PCP
mFluor Violet 450 SE	NB500-430MFV450
mFluor Violet 500 SE	NB500-430MFV500
mFluor Violet 610 SE	NB500-430MFV610

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NBP2-66820

EMMPRIN/CD147 Antibody (JF1-045)

(1 Review)

100 ul

NBP2-66820

Immunocytochemistry/Immunofluorescence: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820] - Staining CD147 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.

Immunocytochemistry/Immunofluorescence: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820] - Staining CD147 in HeLa cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820] - Analysis of paraffin-embedded human colon cancer tissue using anti-CD147 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820] - Analysis of paraffin-embedded mouse brain tissue using anti-CD147 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820] - Analysis of paraffin-embedded mouse heart tissue using anti-CD147 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820] - Analysis of paraffin-embedded mouse testis tissue using anti-CD147 antibody. Counter stained with hematoxylin.

Western Blot: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820] - analysis of CD147 on different lysates with Rabbit anti-CD147 antibody at 1/5,000 dilution.Lane 1: HepG2 cell lysate (15 ug/Lane)Lane 2: NCI-H226 cell lysate (15 ug/Lane)Lane 3: 786-0 cell lysate (15 µg/Lane)Lane 4: RAW264.7 cell lysate (15 ug/Lane)Lane 5: Mouse liver tissue lysate (20 ug/Lane)Lane 6: Rat liver tissue lysate (20 ug/Lane)Predicted band size: 42 kDaObserved band size: 45-60 kDaExposure time: 3 minutes;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP2-02534

EMMPRIN/CD147 Antibody (OTI9H5)

0.1 ml

NBP2-02534

Immunohistochemistry-Paraffin: CD147 Antibody (9H5) [NBP2-02534] - Staining of paraffin-embedded Human prostate tissue using anti-CD147 mouse monoclonal antibody.

Flow Cytometry: CD147 Antibody (9H5) [NBP2-02534] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CD147 antibody, and then analyzed by flow cytometry.

Western Blot: CD147 Antibody (9H5) [NBP2-02534] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD147 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD147.

Western Blot: CD147 Antibody (9H5) [NBP2-02534] Analysis of extracts (35ug) from 9 different cell lines by using anti-CD147 monoclonal antibody.

Immunocytochemistry/Immunofluorescence: CD147 Antibody (9H5) [NBP2-02534] - Immunofluorescent staining of HT29 cells using anti-CD147 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: CD147 Antibody (9H5) [NBP2-02534] - Staining of paraffin-embedded Human Kidney tissue using anti-CD147 mouse monoclonal antibody.

Reactivity:

Hu, Rt, Ca, Pm, Pm

Species Glossary

Reactivity: Hu, Rt, Ca, Pm, Pm

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-70278AF350
Alexa Fluor 405	NBP2-70278AF405
Alexa Fluor 488	NBP2-70278AF488
Alexa Fluor 532	NBP2-70278AF532
Alexa Fluor 594	NBP2-70278AF594
Alexa Fluor 647	NBP2-70278AF647
Alexa Fluor 700	NBP2-70278AF700
Alexa Fluor 750	NBP2-70278AF750
Allophycocyanin	NBP2-70278APC
Biotin	NBP2-70278B
CoraFluor 1	NBP2-70278CL1
DyLight 350	NBP2-70278UV
DyLight 405	NBP2-70278V
DyLight 488	NBP2-70278G
DyLight 550	NBP2-70278R
DyLight 594	NBP2-70278DL594
DyLight 650	NBP2-70278C
DyLight 680	NBP2-70278FR
DyLight 755	NBP2-70278IR
FITC	NBP2-70278F
HRP	NBP2-70278H
Janelia Fluor 525	NBP2-70278JF525
Janelia Fluor 549	NBP2-70278JF549
Janelia Fluor 646	NBP2-70278JF646
Janelia Fluor 669	NBP2-70278JF669
PE	NBP2-70278PE
PerCP	NBP2-70278PCP
mFluor Violet 450 SE	NBP2-70278MFV450
mFluor Violet 500 SE	NBP2-70278MFV500
mFluor Violet 610 SE	NBP2-70278MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-70278

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NBP2-89320

EMMPRIN/CD147 Antibody (125)

100 ul

NBP2-89320

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Staining of human CD147 in human esophagus with rabbit monoclonal antibody (1:5000). The image showing membrane staining of squamous epithelium cell. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

Flow Cytometry: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Analysis of anti-CD147 reactivity on HeLa cells.

Immunocytochemistry/Immunofluorescence: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Staining of human CD147 in HeLa cells with rabbit monoclonal antibody (1:200). The image showing membrane staining of HeLa cells.

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Staining of human CD147 in human stomach with rabbit monoclonal antibody (1:5000). The image showing membrane staining of epithelium cell.

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Staining of human CD147 in human rectal cancer with rabbit monoclonal antibody (1:5000). The image showing membrane staining of epithelium cell in intestinal gland.

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Staining of human CD147 in human placenta with rabbit monoclonal antibody (1:5000). The image showing positive staining of trophoblast.

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Staining of human CD147 in human liver with rabbit monoclonal antibody (1:5000). The image showing membrane staining of hepatocyte.

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Staining of human CD147 in human gastric cancer with rabbit monoclonal antibody (1:5000). The image showing membrane staining of epithelium cell.

Immunoprecipitation: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - Lane A:0.5 mg K562 Whole Cell Lysate 2 uL anti-CD147 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CD147 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 42 kDaObserved band size: 50 kDa

<b>Genetic Strategies Validation. </b>Knockout Validated: EMMPRIN/CD147 Antibody (125) [NBP2-89320] - NBP2-89320 at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: CD147 knockout Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:42 kDa. Observed band size:35-55 kDa

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-89320AF350
Alexa Fluor 405	NBP2-89320AF405
Alexa Fluor 488	NBP2-89320AF488
Alexa Fluor 532	NBP2-89320AF532
Alexa Fluor 594	NBP2-89320AF594
Alexa Fluor 647	NBP2-89320AF647
Alexa Fluor 700	NBP2-89320AF700
Alexa Fluor 750	NBP2-89320AF750
Allophycocyanin	NBP2-89320APC
Biotin	NBP2-89320B
CoraFluor 1	NBP2-89320CL1
DyLight 350	NBP2-89320UV
DyLight 405	NBP2-89320V
DyLight 488	NBP2-89320G
DyLight 550	NBP2-89320R
DyLight 594	NBP2-89320DL594
DyLight 650	NBP2-89320C
DyLight 680	NBP2-89320FR
DyLight 755	NBP2-89320IR
FITC	NBP2-89320F
HRP	NBP2-89320H
Janelia Fluor 525	NBP2-89320JF525
Janelia Fluor 549	NBP2-89320JF549
Janelia Fluor 646	NBP2-89320JF646
Janelia Fluor 669	NBP2-89320JF669
PE	NBP2-89320PE
PerCP	NBP2-89320PCP
mFluor Violet 450 SE	NBP2-89320MFV450
mFluor Violet 500 SE	NBP2-89320MFV500
mFluor Violet 610 SE	NBP2-89320MFV610

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MAB9721

EMMPRIN/CD147 Antibody (1036020) [Unconjugated]

100 μg

MAB9721

Western blot shows lysates of human brain (cerebellum), human heart (ventricle), and human liver. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB9721) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for EMMPRIN/CD147 at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

EMMPRIN/CD147 was detected in immersion fixed 789-O Human renal cell carcinoma cell line using Mouse Anti-Human EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB9721) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

EMMPRIN/CD147 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB9721) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in capillaries. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Western blot shows lysates of HEK293T human embryonic kidney cell line and human EMMPRIN knockout HEK293T human embryonic kidney cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB9721) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for EMMPRIN/CD147 at approximately 45-60 kDa (as indicated) in the parental cell line, but is not detectable in knockout cell line. GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB9721-SP

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MAB7721

EMMPRIN/CD147 Antibody (1159A) [Unconjugated]

100 μg

MAB7721

Western blot shows lysates of mouse brain tissue and mouse heart tissue. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Mouse EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB7721) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for EMMPRIN/CD147 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

EMMPRIN/CD147 was detected in perfusion fixed frozen sections of mouse testis using Rabbit Anti-Mouse EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB7721) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to spermatozoa. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

J774A.1 cells were stained with Rabbit Anti-Mouse EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB7721, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins.

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB7721U
Alexa Fluor 405	FAB7721V
Alexa Fluor 488	FAB7721G
Alexa Fluor 532	FAB7721X
Alexa Fluor 594	FAB7721T
Alexa Fluor 647	FAB7721R-025UG
Alexa Fluor 647	FAB7721R-100UG
Alexa Fluor 700	FAB7721N
Alexa Fluor 750	FAB7721S
Allophycocyanin	FAB7721A
Allophycocyanin/Cy7	FAB7721APCCY7
Biotin	FAB7721B
CoraFluor 1	FAB7721CL1
DyLight 350	FAB7721D
DyLight 405	FAB7721E
DyLight 488	FAB7721K
DyLight 550	FAB7721L
DyLight 594	FAB7721M
DyLight 650	FAB7721W
DyLight 680	FAB7721Y
DyLight 755	FAB7721Z
FITC	FAB7721F
HRP	FAB7721H
Janelia Fluor 525	FAB7721JF525
Janelia Fluor 549	FAB7721I
Janelia Fluor 646	FAB7721J
Janelia Fluor 669	FAB7721JF669
PE/Atto594	FAB7721PEATT594
PE/Cy5.5	FAB7721PECY55
PE/Cy7	FAB7721PECY7
PerCP	FAB7721C
Phycoerythrin	FAB7721P-025UG
Phycoerythrin	FAB7721P-100UG
mFluor Violet 450 SE	FAB7721MFV450
mFluor Violet 500 SE	FAB7721MFV500
mFluor Violet 610 SE	FAB7721MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB7721-SP
Carrier Free	FAB7721P-025UG
Carrier Free	FAB7721R-025UG
Carrier Free	FAB7721P-100UG
Carrier Free	FAB7721R-100UG

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MAB9722

EMMPRIN/CD147 Antibody (2821A) [Unconjugated]

100 μg

MAB9722

Western blot shows lysates of Human heart (aorta), human kidney, human liver, human testis, and HEK293T human embryonic kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB9722) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for EMMPRIN/CD147 at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

EMMPRIN/CD147 was detected in immersion fixed WM‑115 human malignant melanoma cell line using Rabbit Anti-Human EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB9722) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

EMMPRIN/CD147 was detected in immersion fixed paraffin-embedded sections of human pancreas using Rabbit Anti-Human EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB9722) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in exocrine cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB9722-SP

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NB100-65518

EMMPRIN/CD147 Antibody (OX114)

0.125 mg

NB100-65518

Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) [NB100-65518] - Staining of mouse peripheral blood lymphocytes with Rat anti MouseE CD147: FITC.

Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) [NB100-65518] - Staining of mouse spleen cells with Rat anti Mouse CD147: Alexa Fluor 488.

Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) [NB100-65518] - Analysis using the Biotin conjugate of NB100-65518. Staining of mouse spleen cells with RAT ANTI MOUSE CD147: BIOTIN.

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-65518AF350
Alexa Fluor 405	NB100-65518AF405
Alexa Fluor 488	NB100-65518AF488
Alexa Fluor 532	NB100-65518AF532
Alexa Fluor 594	NB100-65518AF594
Alexa Fluor 647	NB100-65518AF647
Alexa Fluor 700	NB100-65518AF700
Alexa Fluor 750	NB100-65518AF750
Allophycocyanin	NB100-65518APC
Biotin	NB100-65518B
DyLight 350	NB100-65518UV
DyLight 405	NB100-65518V
DyLight 488	NB100-65518G
DyLight 550	NB100-65518R
DyLight 594	NB100-65518DL594
DyLight 650	NB100-65518C
DyLight 680	NB100-65518FR
DyLight 755	NB100-65518IR
FITC	NB100-65518F
HRP	NB100-65518H
Janelia Fluor 549	NB100-65518JF549
Janelia Fluor 646	NB100-65518JF646
PE	NB100-65518PE
PerCP	NB100-65518PCP

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

EMMPRIN/CD147 Antibodies

EMMPRIN/CD147 Antibody [Unconjugated]

EMMPRIN/CD147 Antibody (OX-47) - BSA Free

EMMPRIN/CD147 Antibody (109403) [Unconjugated]

EMMPRIN/CD147 Antibody [Unconjugated]

EMMPRIN/CD147 Antibody (MEM-M6/1) - BSA Free

EMMPRIN/CD147 Antibody (JF1-045)

EMMPRIN/CD147 Antibody (OTI9H5)

EMMPRIN/CD147 Antibody (125)

EMMPRIN/CD147 Antibody (1036020) [Unconjugated]

EMMPRIN/CD147 Antibody (1159A) [Unconjugated]

EMMPRIN/CD147 Antibody (2821A) [Unconjugated]

EMMPRIN/CD147 Antibody (OX114)