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EMMPRIN/CD147 Antibody (OX114)

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Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) [NB100-65518] - Analysis using the FITC conjugate of NB100-65518. Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD147: FITC.
Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) [NB100-65518] - Analysis using the FITC conjugate of NB100-65518. Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD147: FITC.
Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) [NB100-65518] - Staining of mouse peripheral blood lymphocytes with RAT ANTI MOUSE CD147.
Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) [NB100-65518] - Staining of mouse peripheral blood lymphocytes with Rat anti MouseE CD147: FITC.
Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) [NB100-65518] - Staining of mouse spleen cells with Rat anti Mouse CD147: Alexa Fluor 488.
Flow Cytometry: EMMPRIN/CD147 Antibody (OX114) [NB100-65518] - Analysis using the Biotin conjugate of NB100-65518. Staining of mouse spleen cells with RAT ANTI MOUSE CD147: BIOTIN.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC
Clone
OX114
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-65518AF350
Alexa Fluor 405
NB100-65518AF405
Alexa Fluor 488
NB100-65518AF488
Alexa Fluor 532
NB100-65518AF532
Alexa Fluor 594
NB100-65518AF594
Alexa Fluor 647
NB100-65518AF647
Alexa Fluor 700
NB100-65518AF700
Alexa Fluor 750
NB100-65518AF750
Allophycocyanin
NB100-65518APC
Biotin
NB100-65518B
DyLight 350
NB100-65518UV
DyLight 405
NB100-65518V
DyLight 488
NB100-65518G
DyLight 550
NB100-65518R
DyLight 594
NB100-65518DL594
DyLight 650
NB100-65518C
DyLight 680
NB100-65518FR
DyLight 755
NB100-65518IR
FITC
NB100-65518F
HRP
NB100-65518H
Janelia Fluor 549
NB100-65518JF549
Janelia Fluor 646
NB100-65518JF646
PE
NB100-65518PE
PerCP
NB100-65518PCP
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EMMPRIN/CD147 Antibody (OX114) Summary

Immunogen
Fusion protein mCD147dO-2rCD4d3 +4
Specificity
NB100-65518 recognizes mouse CD147 (also known as basigin). CD147 is a cell surface glycoprotein that is widely expressed on leukocytes, red cells, platelets and endothelial cells. The reported functions of CD147 are diverse and include involvement in T-cell activation, cell adhesion and induction of matrix metalloproteinases. OX-114 recognizes both isoforms of CD147, the common form containing 2 Ig-like domains and the rare form containing 3 Ig-like domains. This antibody is routinely tested in flow cytometry on mouse splenocytes. Note that for staining of whole blood samples increased amounts of antibody may be required due to the presence of CD147 on erythrocytes.
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
BSG
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:25-1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for EMMPRIN/CD147 Antibody (OX114)

  • 5F7
  • basigin (Ok blood group)
  • Basigin
  • BSG
  • CD147 antigen
  • CD147
  • Collagenase stimulatory factor
  • EMMPRIN
  • EMMPRINTCSF
  • Extracellular matrix metalloproteinase inducer
  • Leukocyte activation antigen M6
  • M6
  • OK blood group antigen
  • OK
  • Tumor cell-derived collagenase stimulatory factor

Background

CD147 plays a pivotal role in spermatogenesis, embryo implantation, neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). CD147 may target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. CD147 also seems to be a receptor for oligomannosidic glycans. In vitro, it promotes outgrowth of astrocytic processes. CD147 forms homooligomers in a cis-dependent manner on the plasma membrane. It forms a complex with MMP1 at the tumor cell surface. CD147 interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. CD147 also interacts with ATP1B2, MAG and L1CAM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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EMMPRIN/CD147
Antibody (OX114)
NB100-65518
EMMPRIN/CD147 Antibody (OX114)
Species: Mu
Applications: WB, Flow, IHC

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Bioinformatics

Gene Symbol BSG
Uniprot