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eIF4E Antibody (299910) [Unconjugated]

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eIF4E was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Mouse Anti-Human/Mouse/Rat eIF4E Monoclonal Antibody (Catalog # MAB3228) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
eIF4E was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Mouse Anti-Human/Mouse/Rat eIF4E Monoclonal Antibody (Catalog # MAB3228) for 3 hours at room temperature. Cells were stained with ...read more
Western blot shows lysates of MCF-7 human breast cancer cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse ...read more
Genetic Strategies: Western blot shows lysates of MCF-7 human breast cancer parental cell line and eIF4E knockout MCF-7 cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat eIF4E ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, KO
Clone
299910
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3228-SP
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eIF4E Antibody (299910) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human eIF4E
Met1-Val217
Accession # P06730
Specificity
Detects human, mouse and rat eIF4E.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
EIF4E
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Knockout Validated
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
MAB3228 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for eIF4E Antibody (299910) [Unconjugated]

  • CBP
  • eIF4E
  • eIF-4E
  • EIF4E1
  • EIF4EL1
  • EIF4EL1MGC111573
  • eIF-4F 25 kDa subunit
  • EIF4F
  • eukaryotic translation initiation factor 4E
  • eukaryotic translation initiation factor 4E-like 1
  • mRNA cap-binding protein

Background

eIF4E (eukaryotic initiation factor 4E) interacts with the 7-methyl-GTP cap structure to facilitate the initiation and rate of translation of mRNA. Together with eIF4G and eIF4A, it forms the eIF4F complex. eIF4E activity has been shown to play a role in cell cycle progression, tumorigenesis, embryonic development, nuclear export and synaptic plasticity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for eIF4E Antibody (MAB3228)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


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Bioinformatics

Gene Symbol EIF4E
Uniprot