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![Western Blot: eIF4E [p Ser209] Antibody [NB100-81898] - Analysis of extracts from SK-BR-3 cells, untreated or insulin and EGF treated, and pretreated with U0126 cells, using NB100-81898](http://images.novusbio.com/fullsize/eIF4E-[p-Ser209]-Antibody-Western-Blot-NB100-81898-img0009.jpg "Western Blot: eIF4E [p Ser209] Antibody [NB100-81898] - Analysis of extracts from SK-BR-3 cells, untreated or insulin and EGF treated, and pretreated with U0126 cells, using NB100-81898")
![Immunocytochemistry/Immunofluorescence: eIF4E [p Ser209] Antibody [NB100-81898] - Staining of methanol-fixed MCF cells using NB100-81898](http://images.novusbio.com/fullsize/eIF4E-[p-Ser209]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-81898-img0005.jpg)
![Immunohistochemistry-Paraffin: eIF4E [p Ser209] Antibody [NB100-81898] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81898 (left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/eIF4E-[p-Ser209]-Antibody-Immunohistochemistry-NB100-81898-img0006.jpg)
![Western Blot: eIF4E Antibody [NB100-58833] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF4E antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Western-Blot-NB100-58833-img0002.jpg "Western Blot: eIF4E Antibody [NB100-58833] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF4E antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: eIF4E Antibody [NB100-58833] - Detection of human eIF4E by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF4E antibody NB100-58833 used for IP at 3 ug per reaction. eIF4E was also immunoprecipitated by another rabbit anti-eIF4E antibody. For blotting immunoprecipitated eIF4E, NB100-58833 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Immunoprecipitation-NB100-58833-img0003.jpg)
![Western Blot: eIF4E Antibody (5D11) [NBP2-22177] - Western blot analysis using eIF4E mouse mAb against Hela (1), HEK293 (2) and K562 (3) cell lysate.](http://images.novusbio.com/fullsize/eIF4E-Antibody-5D11-Western-Blot-NBP2-22177-img0006.jpg "Western Blot: eIF4E Antibody (5D11) [NBP2-22177] - Western blot analysis using eIF4E mouse mAb against Hela (1), HEK293 (2) and K562 (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: eIF4E Antibody (5D11) [NBP2-22177] - Immunofluorescence analysis of GC-7901 cells using eIF4E mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/eIF4E-Antibody-5D11-Immunocytochemistry-Immunofluorescence-NBP2-22177-img0002.jpg)
![Immunohistochemistry-Paraffin: eIF4E Antibody (5D11) [NBP2-22177] - Immunohistochemical analysis of paraffin-embedded stomach cancer (left) and prostate cancer (right) using eIF4E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/eIF4E-Antibody-5D11-Immunohistochemistry-Paraffin-NBP2-22177-img0004.jpg)
![Flow Cytometry: eIF4E Antibody (5D11) [NBP2-22177] - Flow cytometric analysis of Hela cells using EIF4E mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/eIF4E-Antibody-5D11-Flow-Cytometry-NBP2-22177-img0001.jpg)
![Immunohistochemistry-Paraffin: eIF4E Antibody (5D11) [NBP2-22177] - Immunohistochemical analysis of paraffin-embedded liver cancer (left) and submaxillary tumor (right) using eIF4E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/eIF4E-Antibody-5D11-Immunohistochemistry-Paraffin-NBP2-22177-img0003.jpg)
![ELISA: eIF4E Antibody (5D11) [NBP2-22177] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/eIF4E-Antibody-5D11-ELISA-NBP2-22177-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells using eIF4E Mouse Monoclonal Antibody (PCRP-EIF4E-1D3) followed by goat anti- Mouse IgG-CF488 (green), phalloidin (red).](http://images.novusbio.com/fullsize/eIF4E-Antibody-PCRP-EIF4E-1D3-Immunocytochemistry-Immunofluorescence-NBP3-07141-img0006.jpg "Immunocytochemistry/Immunofluorescence: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells using eIF4E Mouse Monoclonal Antibody (PCRP-EIF4E-1D3) followed by goat anti- Mouse IgG-CF488 (green), phalloidin (red).")
![Flow Cytometry: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141] - Flow cytometric analysis of PFA-fixed U87 cells. eIF4E antibody (PCRP-EIF4E-1D3) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).](http://images.novusbio.com/fullsize/eIF4E-Antibody-PCRP-EIF4E-1D3-Flow-Cytometry-NBP3-07141-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141] - Immunofluorescence Analysis of PFA-fixed HeLa cells using eIF4E Mouse Monoclonal Antibody (PCRP-EIF4E-1D3) followed by goat anti- Mouse IgG-CF488 (green).](http://images.novusbio.com/fullsize/eIF4E-Antibody-PCRP-EIF4E-1D3-Immunocytochemistry-Immunofluorescence-NBP3-07141-img0005.jpg)
![Flow Cytometry: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141] - Flow cytometric analysis of PFA-fixed HeLa cells. eIF4E Mouse Monoclonal Antibody (PCRP-eIF4E-1D3) followed by goat anti- Mouse IgG-CF488 (blue), isotype control (red).](http://images.novusbio.com/fullsize/eIF4E-Antibody-PCRP-EIF4E-1D3-Flow-Cytometry-NBP3-07141-img0002.jpg)
![Flow Cytometry: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141] - Flow cytometric analysis of PFA-fixed Raji cells.eIF4E Mouse Monoclonal Antibody (PCRP-eIF4E-1D3) followed by goat anti- Mouse IgG-CF488 (blue), isotype control (red).](http://images.novusbio.com/fullsize/eIF4E-Antibody-PCRP-EIF4E-1D3-Flow-Cytometry-NBP3-07141-img0003.jpg)
![Protein Array: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141] - Analysis of Protein Array containing more than 19,000 full-length human proteins using eIF4E Mouse Monoclonal Antibody (PCRP-eIF4E-1D3).](http://images.novusbio.com/fullsize/eIF4E-Antibody-PCRP-EIF4E-1D3-Protein-Array-NBP3-07141-img0001.jpg)
![Western Blot: eIF4E Antibody (JE60-70) [NBP3-32302] - Western blot analysis of eIF4E on different lysates with Rabbit anti-eIF4E antibody (NBP3-32302) at 1/500 dilution. Lane 1: HL-60 cell lysate Lane 2: Daudi cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32302) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32302_rabbit-eif4e-mab-je60-70-278202415212480.jpg "Western Blot: eIF4E Antibody (JE60-70) [NBP3-32302] - Western blot analysis of eIF4E on different lysates with Rabbit anti-eIF4E antibody (NBP3-32302) at 1/500 dilution. Lane 1: HL-60 cell lysate Lane 2: Daudi cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32302) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: eIF4E Antibody (JE60-70) [NBP3-32302] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-eIF4E antibody (NBP3-32302) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32302) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32302_rabbit-eif4e-mab-je60-70-298202414161811.jpg)
![Immunocytochemistry/ Immunofluorescence: eIF4E Antibody (JE60-70) [NBP3-32302] - Immunocytochemistry analysis of SK-Br-3 cells labeling eIF4E with Rabbit anti-eIF4E antibody (NBP3-32302) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-eIF4E antibody (NBP3-32302) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32302_rabbit-eif4e-mab-je60-70-2920241192325.jpg)
![Flow Cytometry: eIF4E Antibody (JE60-70) [NBP3-32302] - Flow cytometric analysis of SK-Br-3 cells labeling eIF4E. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32302, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32302_rabbit-eif4e-mab-je60-70-29202416453285.jpg)
![Western Blot: eIF4E Antibody [NBP1-57195] - C-terminal region validated by WB using Mouse Brain lysate.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Western-Blot-NBP1-57195-img0005.jpg "Western Blot: eIF4E Antibody [NBP1-57195] - C-terminal region validated by WB using Mouse Brain lysate.")
![Immunohistochemistry-Paraffin: eIF4E Antibody [NBP1-57195] - Human Lung respiratory epithelium tissue, 5ug/ml.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Immunohistochemistry-Paraffin-NBP1-57195-img0004.jpg)
![Western Blot: eIF4E Antibody [NBP1-57195]](http://images.novusbio.com/fullsize/eIF4E-Antibody-Western-Blot-NBP1-57195-img0002.jpg)
![Western Blot: eIF4E Antibody [NBP1-57195] - HEK293 lysate, mouse brain extract, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/eIF4E-Antibody-Western-Blot-NBP1-57195-img0003.jpg)
![Immunohistochemistry: eIF4E [p Ser 209] Antibody (SR1401) [NBP3-21524] - Immunohistochemical analysis of paraffin-embedded mouse kidney, using NBP3-21524.](http://images.novusbio.com/fullsize/nbp3-21524_rabbit-eif4e-p-ser-209-mab-sr1401-5720231754887.jpg "Immunohistochemistry: eIF4E [p Ser 209] Antibody (SR1401) [NBP3-21524] - Immunohistochemical analysis of paraffin-embedded mouse kidney, using NBP3-21524.")
![Western Blot: eIF4E [p Ser 209] Antibody (SR1401) [NBP3-21524] - Western blot analysis using NBP3-21524 in (1) HEK293 cell lysate; (2) Mouse spleen lysate.](http://images.novusbio.com/fullsize/nbp3-21524_rabbit-eif4e-p-ser-209-mab-sr1401-6720239533793.jpg)
![Western Blot: eIF4E Antibody [NBP2-57098] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Western-Blot-NBP2-57098-img0002.jpg "Western Blot: eIF4E Antibody [NBP2-57098] - Analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: eIF4E Antibody [NBP2-57098] - Staining of human cell line U-2 OS shows localization to cytosol & cytoplasmic bodies. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57098-img0001.jpg)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil](http://images.novusbio.com/fullsize/nbp3-21317_rabbit-eif4e-pab-4520231553437.jpg)
![Immunohistochemistry: eIF4E [p Ser209] Antibody (1F3) [NBP3-26426] - Image of eIF4E [p Ser209] Antibody (1F3) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26426_rabbit-eif4e-p-ser-209-mab-1f3-262202414471518.jpg "Immunohistochemistry: eIF4E [p Ser209] Antibody (1F3) [NBP3-26426] - Image of eIF4E [p Ser209] Antibody (1F3) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: eIF4E [p Ser209] Antibody (1F3) [NBP3-26426] - Positive Western Blot detected in 293 whole cell lysate, HepG2 whole cell lysate (treated with Calyculin A or not). All lanes: eIF4E [p Ser209] Antibody at 1.2ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 25 KDa Observed band size: 25 KDa](http://images.novusbio.com/fullsize/nbp3-26426_rabbit-eif4e-p-ser-209-mab-1f3-282202411472143.jpg)
![Western Blot: eIF4E Antibody (7A7P2) [NBP3-15629] - Western blot analysis of extracts of various cell lines, using eIF4E antibody (NBP3-15629) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/eIF4E-Antibody-7A7P2-Western-Blot-NBP3-15629-img0001.jpg "Western Blot: eIF4E Antibody (7A7P2) [NBP3-15629] - Western blot analysis of extracts of various cell lines, using eIF4E antibody (NBP3-15629) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunoprecipitation: eIF4E Antibody (7A7P2) [NBP3-15629] - Immunoprecipitation analysis of 600ug extracts of Mouse testis using 3ug eIF4E antibody (NBP3-15629). Western blot was performed from the immunoprecipitate using eIF4E (NBP3-15629) at a dilition of 1:500.](http://images.novusbio.com/fullsize/eIF4E-Antibody-7A7P2-Immunoprecipitation-NBP3-15629-img0002.jpg)