![Western Blot: DNMT3A Antibody (64B1446) - Azide and BSA Free [NBP2-80699] - NTERA-2 cells were treated with Zebularine as indicated for 24 hours. Cell lysates were prepared and separated on a 7.5% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was probed with 2 ug/mL anti-Dnmt3a in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Note the decrease in Dnmt3a expression upon treatment with 500 uM Zebularine (arrowhead). Additional bands at 90 and 75 kDa can also be detected with this antibody and may represent alternative splice variants. Tubulin is shown as a loading control. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B1446-Azide-and-BSA-Free-Western-Blot-NBP2-80699-img0002.jpg "Western Blot: DNMT3A Antibody (64B1446) - Azide and BSA Free [NBP2-80699] - NTERA-2 cells were treated with Zebularine as indicated for 24 hours. Cell lysates were prepared and separated on a 7.5% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was probed with 2 ug/mL anti-Dnmt3a in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Note the decrease in Dnmt3a expression upon treatment with 500 uM Zebularine (arrowhead). Additional bands at 90 and 75 kDa can also be detected with this antibody and may represent alternative splice variants. Tubulin is shown as a loading control. Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ChIP, Flow, ICC/IF, IHC, ChIP, CyTOF-ready, IHC |
| Clone | 64B1446 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | This DNMT3A Antibody (64B1446) was raised against bacteria expressed recombinant mouse Dnmt3a. The epitope was found to lie near the C-terminus (a.a. 705-908), see Chen et (2002) for details. |
| Localization | Nuclear |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | DNMT3A |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Western Blot: Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa). Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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Chromatin reader domains of DNMT-targeting protein, UHRF1, are responsible for cancerous DNA hypermethylation By Jamshed Arslan, Pharm. D., PhD. DNA methylation represses transcription of many genes, including tumor suppressor genes. A protein called UHRF1 recruits DNA methyltransferases (DNMTs) to establish and maintain DNA... Read full blog post. |
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Go Ahead! Make My DNA DNA methylation plays a critical role the long-term silencing of transcription and is essential for processes such as embryonic development, germline differentiation, and tissue maturation. Dnmt3a is a member of the C5-methyltransferase family that re... Read full blog post. |
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DNMT's: An Overview of 3 DNA Methyltransferases DNA methyltransferases catalyze the transfer of the methyl group from S-andenosyl methionine (SAM) to DNA. Such methylation has wide ranging function in the cell, including organismal development and cell differentiation. In cancer, abnormal hypermeth... Read full blog post. |
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| Gene Symbol | DNMT3A |
![Immunocytochemistry/Immunofluorescence: DNMT3A Antibody (64B1446) - Azide and BSA Free [NBP2-80699] - Ntera2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-DNMT3A (68B1446) [NB120-13888] at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B1446-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80699-img0005.jpg "Immunocytochemistry/Immunofluorescence: DNMT3A Antibody (64B1446) - Azide and BSA Free [NBP2-80699] - Ntera2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-DNMT3A (68B1446) [NB120-13888] at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.")
![Immunohistochemistry: DNMT3A Antibody (64B1446) - Azide and BSA Free [NBP2-80699] - Detection of DNMT3A on human hepatocellular carcinoma tissue section (NBP2-30221) using 1:100 dilution of DNMT3A antibody (clone 64B1446). The antibody generated a specific cytoplasmic staining in all the cancer cells while some of the cells depicted nucl](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B1446-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80699-img0006.jpg "Immunohistochemistry: DNMT3A Antibody (64B1446) - Azide and BSA Free [NBP2-80699] - Detection of DNMT3A on human hepatocellular carcinoma tissue section (NBP2-30221) using 1:100 dilution of DNMT3A antibody (clone 64B1446). The antibody generated a specific cytoplasmic staining in all the cancer cells while some of the cells depicted nucl")
![Flow Cytometry: DNMT3A Antibody (64B1446) - Azide and BSA Free [NBP2-80699] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Dnmt3a antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B1446-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80699-img0011.jpg "Flow Cytometry: DNMT3A Antibody (64B1446) - Azide and BSA Free [NBP2-80699] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Dnmt3a antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.")
![Western Blot: DNMT3A Antibody (64B1446) - Azide and BSA Free [NBP2-80699] - Analysis of (A) Dnmt3a transfected 293 cell lysate and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/mL. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B1446-Azide-and-BSA-Free-Western-Blot-NBP2-80699-img0001.jpg "Western Blot: DNMT3A Antibody (64B1446) - Azide and BSA Free [NBP2-80699] - Analysis of (A) Dnmt3a transfected 293 cell lysate and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/mL. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: DNMT3A Antibody (64B1446) - Azide and BSA Free [NBP2-80699] - DNMT1 reveals a SCMC-like cortical localization with aberrant localization in Nlrp2tm1a/tm1a derived oocytes. Whole mount immunofluorescence for DNMT3A in unfertilized control oocytes reveals a characteristic metaphase associated localization and no difference is noted in oocytes derived from Nlrp2tm1a/tm1adams. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep44667), licensed under a CC-BY license. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B1446-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80699-img0003.jpg "Immunocytochemistry/Immunofluorescence: DNMT3A Antibody (64B1446) - Azide and BSA Free [NBP2-80699] - DNMT1 reveals a SCMC-like cortical localization with aberrant localization in Nlrp2tm1a/tm1a derived oocytes. Whole mount immunofluorescence for DNMT3A in unfertilized control oocytes reveals a characteristic metaphase associated localization and no difference is noted in oocytes derived from Nlrp2tm1a/tm1adams. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep44667), licensed under a CC-BY license. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: DNMT3A Antibody (64B1446) - Azide and BSA Free [NBP2-80699] - Expression of Dnmt3a in the nuclei of post-mitotic neurons in the olfactory epithelium (OE). DNMT3a (red) is co-expressed with neuron-specific tubulin (green) throughout the development of the olfactory epithelium. Data courtesy of A. Jane Roskams, University of British Columbia. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B1446-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80699-img0004.jpg "Immunocytochemistry/Immunofluorescence: DNMT3A Antibody (64B1446) - Azide and BSA Free [NBP2-80699] - Expression of Dnmt3a in the nuclei of post-mitotic neurons in the olfactory epithelium (OE). DNMT3a (red) is co-expressed with neuron-specific tubulin (green) throughout the development of the olfactory epithelium. Data courtesy of A. Jane Roskams, University of British Columbia. Image from the standard format of this antibody.")
![Flow Cytometry: DNMT3A Antibody (64B1446) - Azide and BSA Free [NBP2-80699] - An intracellular stain was performed on HeLa cells with DNMT3A (64B1446) NB120-13888APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to allophycocyanin (APC).](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B1446-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80699-img0007.jpg "Flow Cytometry: DNMT3A Antibody (64B1446) - Azide and BSA Free [NBP2-80699] - An intracellular stain was performed on HeLa cells with DNMT3A (64B1446) NB120-13888APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to allophycocyanin (APC).")
![Flow Cytometry: DNMT3A Antibody (64B1446) - Azide and BSA Free [NBP2-80699] - An intracellular stain was performed on HepG2 cells with DNMT3A (64B1446) NB120-13888AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B1446-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80699-img0008.jpg "Flow Cytometry: DNMT3A Antibody (64B1446) - Azide and BSA Free [NBP2-80699] - An intracellular stain was performed on HepG2 cells with DNMT3A (64B1446) NB120-13888AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.")
![Flow Cytometry: DNMT3A Antibody (64B1446) - Azide and BSA Free [NBP2-80699] - An intracellular stain was performed on HepG2 cells with DNMT3A (64B1446) NB120-13888APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to allophycocyanin (APC).](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B1446-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80699-img0009.jpg "Flow Cytometry: DNMT3A Antibody (64B1446) - Azide and BSA Free [NBP2-80699] - An intracellular stain was performed on HepG2 cells with DNMT3A (64B1446) NB120-13888APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to allophycocyanin (APC).")
![Flow Cytometry: DNMT3A Antibody (64B1446) - Azide and BSA Free [NBP2-80699] - An intracellular stain was performed on Jurkat cells with DNMT3A (64B1446) NB120-13888AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B1446-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80699-img0010.jpg "Flow Cytometry: DNMT3A Antibody (64B1446) - Azide and BSA Free [NBP2-80699] - An intracellular stain was performed on Jurkat cells with DNMT3A (64B1446) NB120-13888AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.")
![Flow Cytometry: DNMT3A Antibody (64B1446) - Azide and BSA Free [NBP2-80699] - Using the PE direct conjugate, an intracellular stain was performed on NTERA-2 cells with DNMT3A (64B1446) NB120-13888PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/DNMT3A-Antibody-64B1446-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80699-img0012.jpg "Flow Cytometry: DNMT3A Antibody (64B1446) - Azide and BSA Free [NBP2-80699] - Using the PE direct conjugate, an intracellular stain was performed on NTERA-2 cells with DNMT3A (64B1446) NB120-13888PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to phycoerythrin.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")