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DNA Polymerase iota Antibody

Images

 
Immunohistochemistry-Paraffin: DNA Polymerase iota Antibody [NBP2-55989] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: DNA Polymerase iota Antibody [NBP2-55989] - Staining of human skeletal muscle shows low expression as expected.
Orthogonal Strategies: Immunohistochemistry-Paraffin: DNA Polymerase iota Antibody [NBP2-55989] - Staining in human testis and skeletal muscle tissues using anti-POLI antibody. Corresponding POLI RNA-seq data are ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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DNA Polymerase iota Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: PKDKETNRDFLPSGRIESTRTRESPLDTTNFSKEKDINEFPLCSLPEGVDQEVFKQLPVDIQEEILSGKSREKFQGKGSVSCPL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLI
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
DNA Polymerase iota Recombinant Protein Antigen (NBP2-55989PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for DNA Polymerase iota Antibody

  • DNA polymerase iota
  • EC 2.7.7.7
  • eta2
  • polymerase (DNA directed) iota
  • RAD30 homolog B
  • RAD30Bpolymerase (DNA-directed), iota
  • RAD3OB

Background

DNA polymerase Iota (POLI), also known as Rad30B is a novel human polymerase, unlike any others known. It is an extremely low-fidelity enzyme that is highly error-prone when replicating undamaged and damaged DNA in vitro. To date there is, however, no human disease that can be attributed to overexpression or defects in DNA Pol I.

DNA Pol I antibodies are useful for DNA replication and repair studies and in research on mitosis and cell replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol POLI