Western Blot: AP2M1 Antibody (1E9) [NBP2-00834] Analysis of extracts (35ug) from 9 different cell lines by using anti-AP2M1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: AP2M1 Antibody (1E9) [NBP2-00834] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY AP2M1.
Immunohistochemistry-Paraffin: AP2M1 Antibody (1E9) [NBP2-00834] - Staining of paraffin-embedded Human colon tissue using anti-AP2M1 mouse monoclonal antibody.
Western Blot: AP2M1 Antibody (1E9) [NBP2-00834] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AP2M1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
49.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-00834 in the following applications:
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Packaging, Storage & Formulations
Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.65 mg/ml
Purity
Immunogen affinity purified
Alternate Names for AP2M1 Antibody (OTI1E9)
Adaptin-mu2
adaptor-related protein complex 2, mu 1 subunit
AP-2 mu chain
Clathrin assembly protein complex 2 medium chain
clathrin coat adaptor protein AP50
Clathrin coat assembly protein AP50
Clathrin coat-associated protein AP50
clathrin-associated/assembly/adaptor protein, medium 1
HA2 50 kDa subunit
KIAA0109
mu subunit
Plasma membrane adaptor AP-2 50 kDa protein
plasma membrane adaptor AP-2 50kDA protein
Background
This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for AP2M1 Antibody (NBP2-00834). (Showing 1 - 1 of 1 FAQ).
I have a question about your product ,named AP2M1 antibody (1E9) NBP2-00834. I didn't find the concentration of this anbody on the product page, could you please tell me the concentrarion infomation of this antibody?
Current lot of AP2M1 antibody NBP2-00834 is supplied at 0.65 mg/ml. The vial is 100ul.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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