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XPV/DNA polymerase eta Antibody

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Western blot shows lysates of MRC-5 human embryonic lung fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human XPV Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3417) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
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XPV/DNA polymerase eta Antibody Summary

Immunogen
E. coli-derived recombinant human XPV
Met1-Phe400
Accession # Q9Y253
Specificity
Detects human XPV in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
POLH
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for XPV/DNA polymerase eta Antibody

  • DNA polymerase eta
  • DNA Polymerase, eta
  • EC 2.7.7.7
  • POLH
  • polymerase (DNA directed), eta
  • RAD30 homolog A
  • RAD30
  • RAD30A
  • RAD30AFLJ16395
  • Xeroderma pigmentosum variant type protein
  • XPV
  • XP-V
  • XPVFLJ21978

Background

Xeroderma pigmentosum variant (XPV) is a form of XP caused by mutation in the DNA polymerase eta (POLH) gene. DNA polymerase eta catalyzes translesion DNA synthesis past pyrimidine dimers, a mutation caused by exposure to ultraviolet (UV) light. XPV patients are sensitive to UV light and show an increased incidence of skin cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol POLH
Uniprot