![Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - POLK monoclonal antibody (M01), clone 6F2 Analysis of POLK expression in Hela S3 NE.](http://images.novusbio.com/fullsize/DNA-Polymerase-Kappa-Antibody-6F2-Western-Blot-H00051426-M01-img0014.jpg "Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - POLK monoclonal antibody (M01), clone 6F2 Analysis of POLK expression in Hela S3 NE.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, KD |
| Clone | 6F2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | POLK (AAH50718, 1 a.a. ~ 472 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDSTKEKCDSYKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQKAQITSQQLRKAQLQVDRFAMELEQSRNLSNTIVHIDMDAFYAAVEMRDNPELKDKPIAVGSMSMLSTSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILADYDPNFMAMSLDEAYLNITKHLEERQNWPEDKRRYFIKMGSSVENDNPGKEVNKLSEHERSISPLLFEESPSDVQPPGDPFQVNFEEQNNPQILQNSVVFGTSAQEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPNRQAVMDFIKDLPIRKVSGIGKVTEKMLKALGIITCTELYQQRALLSLLFSETSWHYFLHISLGLGSTHLTRDGERKSMSVERTFSEINKAEEQYSLCQELCSELAQDLQKERLKVLYFDMVSLVFKFFNSKMLP |
| Specificity | POLK - polymerase (DNA directed) kappa |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | POLK |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody reactive against recominant protein and cell lysate for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), RNAi validation and ELISA. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
WB | Human | 01/07/2015 |
Summary
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of monoclonal antibody to POLK on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/DNA-Polymerase-Kappa-Antibody-6F2-Immunocytochemistry-Immunofluorescence-H00051426-M01-img0009.jpg "Immunocytochemistry/Immunofluorescence: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of monoclonal antibody to POLK on HeLa cell. Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of monoclonal antibody to POLK on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/DNA-Polymerase-Kappa-Antibody-6F2-Immunohistochemistry-Paraffin-H00051426-M01-img0011.jpg "Immunohistochemistry-Paraffin: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of monoclonal antibody to POLK on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.")
![Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - analysis of DNA Polymerase Kappa in U87MG (lane 1), T98G (lane 2), A172 (lane 3) and normal human astrocyte (lane 4) lystes using anti-DNA Polymerase Kappa antibody. Each lane loaded with 10ug of samples. The primary antibody was used at a dilution of 1:5000 and incubated for 2 hours at room temperature in 0.1 % (w/v) BSA in PBS and 0.1% (v/v) tween-20. Image from verified customer review.](http://images.novusbio.com/fullsize/DNA-Polymerase-Kappa-Antibody-6F2-Western-Blot-H00051426-M01-img0007.jpg "Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - analysis of DNA Polymerase Kappa in U87MG (lane 1), T98G (lane 2), A172 (lane 3) and normal human astrocyte (lane 4) lystes using anti-DNA Polymerase Kappa antibody. Each lane loaded with 10ug of samples. The primary antibody was used at a dilution of 1:5000 and incubated for 2 hours at room temperature in 0.1 % (w/v) BSA in PBS and 0.1% (v/v) tween-20. Image from verified customer review.")
![Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of POLK over-expressed 293 cell line, cotransfected with POLK Validated Chimera RNAi ( Cat # H00051426-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with POLK monoclonal antibody (M01), clone 6F2 (Cat # H00051426-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/DNA-Polymerase-Kappa-Antibody-6F2-Western-Blot-H00051426-M01-img0012.jpg "Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of POLK over-expressed 293 cell line, cotransfected with POLK Validated Chimera RNAi ( Cat # H00051426-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with POLK monoclonal antibody (M01), clone 6F2 (Cat # H00051426-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of POLK expression in transfected 293T cell line by POLK monoclonal antibody (M01), clone 6F2.Lane 1: POLK transfected lysate(54.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/DNA-Polymerase-Kappa-Antibody-6F2-Western-Blot-H00051426-M01-img0015.jpg "Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Analysis of POLK expression in transfected 293T cell line by POLK monoclonal antibody (M01), clone 6F2.Lane 1: POLK transfected lysate(54.1 KDa).Lane 2: Non-transfected lysate.")
![ELISA: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Detection limit for recombinant GST tagged POLK is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/DNA-Polymerase-Kappa-Antibody-6F2-ELISA-H00051426-M01-img0016.jpg "ELISA: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - Detection limit for recombinant GST tagged POLK is approximately 0.3ng/ml as a capture antibody.")
![Western Blot Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) - BSA Free](https://images.novusbio.com/images/Androgen-R-NR3C4-p-Ser213-p-Ser210-Antibody-156C135-2-Western-Blot-NB100-56603-img0004.jpg)
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")
