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Dishevelled-3 Antibody [Unconjugated]

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Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Dishevelled-3 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Dishevelled-3
His545-Gly654
Accession # Q92997
Specificity
Detects human Dishevelled-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Dishevelled-1 and recombinant human Dishevelled-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
DVL3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dishevelled-3 Antibody [Unconjugated]

  • dishevelled 3 (homologous to Drosophila dsh)
  • dishevelled, dsh homolog 3 (Drosophila)
  • Dishevelled3
  • Dishevelled-3
  • DSH homolog 3
  • DVL3
  • KIAA0208dishevelled-3
  • segment polarity protein dishevelled homolog DVL-3

Background

Dishevelled-3 is one of three mammalian multi-moduled Dishevelled proteins that have important roles in the canonical and non-canonical Wnt pathways. Human Dishevelled-3 is a 716 amino acid residue protein with an N-terminal DIX domain, a central PZD domain, and a DEP domain. Over the region used as immunogen, human Dishevelled-3 shares 99% amino acid sequence homology with mouse and canine Dishevelled-3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol DVL3
Uniprot