![Flow Cytometry: Defensin beta 1 Antibody (1F5) [NBP3-26625] - Overlay Peak curve showing HepG2 cells stained with Defensin beta 1 Antibody (1F5) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26625_rabbit-defensin-beta-1-mab-1f5-282202414183637.jpg "Flow Cytometry: Defensin beta 1 Antibody (1F5) [NBP3-26625] - Overlay Peak curve showing HepG2 cells stained with Defensin beta 1 Antibody (1F5) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, Flow |
| Clone | 1F5 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant monoclonal antibody expressed in HEK293F cells |
| Immunogen | A synthesized peptide derived from Human Defensin beta 1 [UniProt P60022] |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | DEFB1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20 to -70C. Avoid freeze-thaw cycles. |
| Buffer | PBS, pH 7.4, 150mM NaCl, and 50% glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Defensin beta 1 Antibody (NBP3-26625)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | DEFB1 |
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