![Immunocytochemistry/Immunofluorescence: DAX1/NR0B1 Antibody [NBP1-32832] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NR0B1 protein stained by NR0B1 antibody diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.](http://images.novusbio.com/fullsize/DAX1-NR0B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32832-img0008.jpg "Immunocytochemistry/Immunofluorescence: DAX1/NR0B1 Antibody [NBP1-32832] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NR0B1 protein stained by NR0B1 antibody diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.")
| Reactivity | Hu, Pm, RMSpecies Glossary |
| Applications | Simple Western, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NR0B1. The exact sequence is proprietary. |
| Localization | Nucleus, Cytoplasm |
| Predicted Species | Chimpanzee (99%), Rhesus Macaque (95%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NR0B1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In Simple Western internal validation: Human fetal adrenal glands as sample; separated by size; antibody dilution of 1:50; observed molecular weight was ~51 kDa;matrix was 12-230 kDa; detected by Chemiluminescence. |
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| Theoretical MW | 52 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Immunogen affinity purified |
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| Publication using NBP1-32832 | Applications | Species |
|---|---|---|
| I Savchuk, ML Morvan, JP Antignac, K Gemzell-Da, B LeBizec, O Soder, K Svechnikov Androgenic potential of human fetal adrenals at the end of the first trimester Endocr Connect, 2017-06-07;0(0):. 2017-06-07 [PMID: 28592511] (Simple Western, Human) | Simple Western | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for DAX1/NR0B1 Antibody (NBP1-32832)Find related products by research area.
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