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ACAD8 Antibody

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Western Blot: ACAD8 Antibody [NBP2-47559] - Analysis in control (vector only transfected HEK293T lysate) and ACAD8 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Western Blot: ACAD8 Antibody [NBP2-47559] - Analysis in control (vector only transfected HEK293T lysate) and ACAD8 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Orthogonal Strategies: Immunohistochemistry-Paraffin: ACAD8 Antibody [NBP2-47559] - Staining in human kidney and lymph node tissues using NBP2-47559 antibody. Corresponding ACAD8 RNA-seq data are presented for ...read more
Western Blot: ACAD8 Antibody [NBP2-47559] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line HEL
Immunohistochemistry-Paraffin: ACAD8 Antibody [NBP2-47559] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: ACAD8 Antibody [NBP2-47559] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: ACAD8 Antibody [NBP2-47559] - Staining of human lymph node shows weak granular cytoplasmic positivity in non-germinal center cells.
Immunohistochemistry-Paraffin: ACAD8 Antibody [NBP2-47559] - Staining of human prostate shows moderate granular cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

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ACAD8 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: LSFGKKEKKVGWNSQPTRAVIFEDCAVPVANRIGSEGQGFLIAVRGLNGGRINIASCSLGAAHASVILTRDHLNVRKQFGEPLASNQYLQFTLADMA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACAD8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
ACAD8 Protein (NBP2-47559PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ACAD8 Antibody

  • ACAD-8
  • Activator-recruited cofactor 42 kDa component
  • Acyl-CoA dehydrogenase family member 8
  • acyl-CoA dehydrogenase family, member 8
  • acyl-Coenzyme A dehydrogenase family, member 8
  • ARC42
  • EC 1.3.99
  • EC 1.3.99.-
  • FLJ22590
  • IBD
  • isobutyryl-CoA dehydrogenase, mitochondrial

Background

Isobutyryl-CoA dehydrogenase (EC 1.3.99), encoded by the ACAD8 gene, catalyzes the third step of the degradation of the branched chain amino acid valine (Nguyen et al., 2002 [PubMed 12359132]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ACAD8