Western Blot: Collagen II Antibody (5B2.5) [NB600-844] - Expression levels of related genes during differentiation induction in the 2D environment. Expression of ACAN, collagen II, collagen X and Runx2 was detected by ...read more
Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) [NB600-844] - Staining results for the process of differentiation induction in the RCCS environment. Collagen II immune-histochemical staining on cells after ...read more
Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) [NB600-844] - Analysis of a FFPE section of human cartilage using 1:200 dilution of Collagen II [5B2.5] antibody (NB600-844). The signal was developed using ...read more
Expression levels of related genes during differentiation induction in the 2D environment. (a) qRT-PCR analysis of Sox9, ACAN & collagen II on days 7, 14 & 21 during induction. (b) qRT-PCR analysis of collagen X, Runx2 ...read more
Expression levels of related genes during differentiation induction in the RCCS environment. (a) qPCR analysis of Sox9, ACAN & collagen II on days 7, 14 & 21 during induction. (b) qRT-PCR analysis of collagen X, Runx2, ...read more
Immunohistochemistry: Collagen II Antibody (5B2.5) - BSA Free [NB600-844] - Staining results for the process of differentiation induction in the RCCS environment. (a) Cellular morphology. (b) Toluidine blue staining on ...read more
Immunohistochemistry: Collagen II Antibody (5B2.5) - BSA Free [NB600-844] - Staining results for the process of differentiation induction in the 2D environment. (a) Toluidine blue staining; (b) Collagen II ...read more
Use in Rabbit reported in scientific literature (PMID:35441114).Rat reactivity reported in scientific literature (PMID: 24349545). Rabbit reactivity reported in scientific literature (PMID: 15621244). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Rabbit reactivity reported in scientific literature (PMID:33120203).
Packaging, Storage & Formulations
Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified
Alternate Names for Collagen II Antibody (5B2.5) - BSA Free
Alpha-1 type II collagen
ANFH
AOM
cartilage collagen
Chondrocalcin
COL11A3
COL2A1
Collagen 2
collagen alpha-1(II) chain
Collagen II
collagen II, alpha-1 polypeptide
collagen type 2
collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphysealdysplasia, congenital)
collagen, type II, alpha 1
Collagen-2
MGC131516
SEDC
Background
Highly specific to type II collagen and shows no cross-reaction with types I, III, IV, V, VI, IX, X, or XI. In western blotting, 5B2.5 MAb reacts with the TC fragment of lathritic type II collagen after digestion with mammalian collagenase. It does not react with pepsin-digested type II collagen.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
He W, Wang W, Deng J et al. L-NMMA AND 1400W, INHIBITORS OF iNOS, ATTENUATE THE INDUCTION OF iNOS AND NO IN PRIMARY RABBIT COSTAL CHONDROCYTES BY FLUORIDE Fluoride 2020-01-01 (WB, Rabbit)
Jiang X, Kong Y, Kuss M et al. 3D bioprinting of multilayered scaffolds with spatially differentiated ADMSCs for rotator cuff tendon-to-bone interface regeneration Applied Materials Today 2022-06-01
Dong Y, Sun X, Zhang Z et al. Regional and sustained dual-release of growth factors from biomimetic tri-layered scaffolds for the repair of large-scale osteochondral defects Applied Materials Today 2020-06-01 (IF/IHC)
Janssen L, Saranya M, Leinonen M et al. Vertically aligned carbon nanotube micropillars induce unidirectional chondrocyte orientation Carbon 2019-11-01 (IF/IHC, Human)
IF/IHC
Human
Luo Z, Liu Z, Yu H et al. Multifunctional mesoporous polydopamine near-infrared photothermal controlled release of kartogenin for cartilage repair Materials & Design 2023-07-01
Zheng Z, Xu J, Tu Z et al. Promoter DNA Hypermethylation of Latexin Promotes Inflammatory Activation of Fibro-Nucleus Pulposus Cells in Intervertebral Disc Degeneration papers.ssrn.com 2023-05-22 (WB, Human)
Wang Y, Zhou Y, Gao X et al. Duhuo Jisheng Decoction Regulates Intracellular Zinc Homeostasis by Enhancing Autophagy via PTEN/Akt/mTOR to Improve Knee Cartilage Degeneration bioRxiv 2023-08-22 (IHC, Rat)
IHC
Rat
Zhang A, Sun L, Chen K et al. 3D printing viscoelastic hydrogel-based scaffolds with a swelling-dependent gate for cartilage injury regeneration Chemical Engineering Journal 2023-11-01 (IHC)
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![Western Blot: Collagen II Antibody (5B2.5) [NB600-844] - Expression levels of related genes during differentiation induction in the 2D environment. Expression of ACAN, collagen II, collagen X and Runx2 was detected by western blotting on day 10 during induction. Image collected and cropped by Citeab from the following publication (Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells following transfection with Indian hedgehog and sonic hedgehog using a rotary cell culture system. Cell Mol Biol Lett (2019)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Collagen-II-Antibody-5B2-5-Western-Blot-NB600-844-img0003.jpg "Western Blot: Collagen II Antibody (5B2.5) [NB600-844] - Expression levels of related genes during differentiation induction in the 2D environment. Expression of ACAN, collagen II, collagen X and Runx2 was detected by western blotting on day 10 during induction. Image collected and cropped by Citeab from the following publication (Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells following transfection with Indian hedgehog and sonic hedgehog using a rotary cell culture system. Cell Mol Biol Lett (2019)) licensed under a CC-BY license.")
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![Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) [NB600-844] - Staining results for the process of differentiation induction in the RCCS environment. Collagen II immune-histochemical staining on cells after inoculation onto slides. Image collected and cropped by Citeab from the following publication (Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells following transfection with Indian hedgehog and sonic hedgehog using a rotary cell culture system. Cell Mol Biol Lett (2019)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Collagen-II-Antibody-5B2-5-Immunohistochemistry-Paraffin-NB600-844-img0002.jpg "Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) [NB600-844] - Staining results for the process of differentiation induction in the RCCS environment. Collagen II immune-histochemical staining on cells after inoculation onto slides. Image collected and cropped by Citeab from the following publication (Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells following transfection with Indian hedgehog and sonic hedgehog using a rotary cell culture system. Cell Mol Biol Lett (2019)) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) [NB600-844] - Analysis of a FFPE section of human cartilage using 1:200 dilution of Collagen II [5B2.5] antibody (NB600-844). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.](http://images.novusbio.com/fullsize/Collagen-II-Antibody-5B2-5-Immunohistochemistry-Paraffin-NB600-844-img0001.jpg "Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) [NB600-844] - Analysis of a FFPE section of human cartilage using 1:200 dilution of Collagen II [5B2.5] antibody (NB600-844). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin.")
![Immunohistochemistry: Collagen II Antibody (5B2.5) - BSA Free [NB600-844] - Staining results for the process of differentiation induction in the RCCS environment. (a) Cellular morphology. (b) Toluidine blue staining on cell-microcarrier complexes. (c) Toluidine blue staining on cells after inoculation onto slides. (d) Collagen II immunohistochemical staining on cell-microcarrier complexes. (e) Collagen II immune-histochemical staining on cells after inoculation onto slides. (f) Annexin V-Cy3 immunofluorescence staining. (Bar = 100 um) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30858866), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-844_mouse-monoclonal-collagen-ii-antibody-5b2-5-310202415384190.jpg "Immunohistochemistry: Collagen II Antibody (5B2.5) - BSA Free [NB600-844] - Staining results for the process of differentiation induction in the RCCS environment. (a) Cellular morphology. (b) Toluidine blue staining on cell-microcarrier complexes. (c) Toluidine blue staining on cells after inoculation onto slides. (d) Collagen II immunohistochemical staining on cell-microcarrier complexes. (e) Collagen II immune-histochemical staining on cells after inoculation onto slides. (f) Annexin V-Cy3 immunofluorescence staining. (Bar = 100 um) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30858866), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunohistochemistry: Collagen II Antibody (5B2.5) - BSA Free [NB600-844] - Staining results for the process of differentiation induction in the 2D environment. (a) Toluidine blue staining; (b) Collagen II immunohistochemical staining; (c) Annexin V-Cy3 immunofluorescence staining. (Bar = 100 um) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30858866), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-844_mouse-monoclonal-collagen-ii-antibody-5b2-5-310202415291926.jpg "Immunohistochemistry: Collagen II Antibody (5B2.5) - BSA Free [NB600-844] - Staining results for the process of differentiation induction in the 2D environment. (a) Toluidine blue staining; (b) Collagen II immunohistochemical staining; (c) Annexin V-Cy3 immunofluorescence staining. (Bar = 100 um) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30858866), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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