![Western Blot: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Cytokeratin 8 monoclonal antibody.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-OTI1B12-Western-Blot-NBP1-48281-img0008.jpg "Western Blot: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Cytokeratin 8 monoclonal antibody.")
| Reactivity | Hu, Mu, Rt, Ca, PmSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | OTI1B12 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E.coli. |
| Marker | Epithelial Cell Marker |
| Specificity | This antibody is specific for Homo sapiens keratin 8 (KRT8). |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT8 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 53.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
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| Publication using NBP1-48281 | Applications | Species |
|---|---|---|
| Hong Y, Shan S, Gu Y et al. Malfunction of airway basal stem cells plays a crucial role in pathophysiology of tracheobronchopathia osteoplastica Nature communications 2022-03-14 [PMID: 35288560] (ICC/IF) | ICC/IF |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
IHC-P | Mouse | 11/12/2021 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 8 Antibody (NBP1-48281)Find related products by research area.
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Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytokeratin 8.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-OTI1B12-Immunocytochemistry-Immunofluorescence-NBP1-48281-img0007.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytokeratin 8.")
![Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Cytokeratin 8 antibody staining of mouse colon epithelium. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-OTI1B12-Immunohistochemistry-Paraffin-NBP1-48281-img0015.jpg "Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Cytokeratin 8 antibody staining of mouse colon epithelium. IHC-P image submitted by a verified customer review.")
![Western Blot: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytokeratin 8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytokeratin 8.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-OTI1B12-Western-Blot-NBP1-48281-img0001.jpg "Western Blot: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytokeratin 8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytokeratin 8.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Immunofluorescent staining of A549 cells using anti-Cytokeratin 8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-OTI1B12-Immunocytochemistry-Immunofluorescence-NBP1-48281-img0002.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Immunofluorescent staining of A549 cells using anti-Cytokeratin 8 mouse monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Immunofluorescent staining of HeLa cells using anti-Cytokeratin 8 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-OTI1B12-Immunocytochemistry-Immunofluorescence-NBP1-48281-img0003.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Immunofluorescent staining of HeLa cells using anti-Cytokeratin 8 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-OTI1B12-Immunohistochemistry-Paraffin-NBP1-48281-img0009.jpg "Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.")
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![Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-OTI1B12-Immunohistochemistry-Paraffin-NBP1-48281-img0011.jpg "Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.")
![Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-OTI1B12-Immunohistochemistry-Paraffin-NBP1-48281-img0012.jpg "Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.")
![Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of paraffin-embedded Human prostate tissue within the normal limits using anti-KRT8 mouse monoclonal antibody at 1:150 dilution](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-OTI1B12-Immunohistochemistry-Paraffin-NBP1-48281-img0013.jpg "Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of paraffin-embedded Human prostate tissue within the normal limits using anti-KRT8 mouse monoclonal antibody at 1:150 dilution")
![Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of paraffin-embedded Human bladder tissue within the normal limits using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-OTI1B12-Immunohistochemistry-Paraffin-NBP1-48281-img0014.jpg "Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of paraffin-embedded Human bladder tissue within the normal limits using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.")
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