![Western Blot: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Antibody (OV-TL12/30).](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-OV-TL12-30-Western-Blot-NBP2-44814-img0008.jpg "Western Blot: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Antibody (OV-TL12/30).")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Simple Western, Flow, ICC/IF, IHC |
| Clone | OV-TL12/30 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47940) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | OTN 11, ovarian carcinoma cell line |
| Localization | Cytoplasmic |
| Marker | Glandular and Transitional Epithelial Marker |
| Specificity | It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This monoclonal antibody is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This monoclonal antibody is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-). |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT7 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30239759). Use in Immunohistochemistry reported in scientific literature (PMID: 30522949). |
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| Theoretical MW | 55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
![Choline Kinase beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Choline Kinase beta [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Immunofluorescent staining of methanol-fixed HeLa cells. Cytokeratin 7 Antibody (OV-TL12/30) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-OV-TL12-30-Immunocytochemistry-Immunofluorescence-NBP2-44814-img0007.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Immunofluorescent staining of methanol-fixed HeLa cells. Cytokeratin 7 Antibody (OV-TL12/30) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.")
![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Paraffin-embedded human salivary gland tissue. Image from verified customer review. Image using the Azide and BSA Free format of this antibody.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-OV-TL12-30-Immunohistochemistry-Paraffin-NBP2-44814-img0005.jpg "Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Paraffin-embedded human salivary gland tissue. Image from verified customer review. Image using the Azide and BSA Free format of this antibody.")
![Flow Cytometry: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Flow Cytometric Analysis of HeLa cells using Cytokeratin 7 Antibody (OV-TL12/30) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-OV-TL12-30-Flow-Cytometry-NBP2-44814-img0006.jpg "Flow Cytometry: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Flow Cytometric Analysis of HeLa cells using Cytokeratin 7 Antibody (OV-TL12/30) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).")
![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Human Lung SCC stained with Cytokeratin 7 Monoclonal Antibody (OV-TL12/30)](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-OV-TL12-30-Immunohistochemistry-Paraffin-NBP2-44814-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Human Lung SCC stained with Cytokeratin 7 Monoclonal Antibody (OV-TL12/30)")
![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Human Ovarian Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (OV-TL12/30)](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-OV-TL12-30-Immunohistochemistry-Paraffin-NBP2-44814-img0002.jpg "Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Human Ovarian Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (OV-TL12/30)")
![Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Simple Western lane view shows a specific band for Cytokeratin 7 in 0.1 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-OV-TL12-30-Simple-Western-NBP2-44814-img0003.jpg "Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Simple Western lane view shows a specific band for Cytokeratin 7 in 0.1 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 7 antibody was used at 1 ug/ml dilution of HeLa lysates(s) respectively.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-OV-TL12-30-Simple-Western-NBP2-44814-img0004.jpg "Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 7 antibody was used at 1 ug/ml dilution of HeLa lysates(s) respectively.")
![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Formalin-fixed, paraffin-embedded human prsotatecarcinoma stained with Biotin-conjugated Cytokeratin 7 Mouse Monoclonal Antibody (OV-TL12/30).](http://images.novusbio.com/fullsize/nbp2-44814_mouse-monoclonal-cytokeratin-7-antibody-ov-tl12-30-17720249362618.jpg "Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Formalin-fixed, paraffin-embedded human prsotatecarcinoma stained with Biotin-conjugated Cytokeratin 7 Mouse Monoclonal Antibody (OV-TL12/30).")
![Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Lane view shows a specific band for Cytokeratin 7 at 5 ug/mL in HeLa cell lysate. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 61 kDa. Image from an internal validation.](http://images.novusbio.com/fullsize/nbp2-44814_mouse-monoclonal-cytokeratin-7-antibody-ov-tl12-30-17720241003210.jpg "Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Lane view shows a specific band for Cytokeratin 7 at 5 ug/mL in HeLa cell lysate. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 61 kDa. Image from an internal validation.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")