![Western Blot: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Analysis of Cytokeratin 7 on different lysates using anti-Cytokeratin 7 antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: HepG2 Lane 3: SK-BR-3 Lane 4: SW1990](http://images.novusbio.com/fullsize/Cytokeratin-7-13-17-18-Antibody-ST50-05-Western-Blot-NBP2-67533-img0007.jpg "Western Blot: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Analysis of Cytokeratin 7 on different lysates using anti-Cytokeratin 7 antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: HepG2 Lane 3: SK-BR-3 Lane 4: SW1990")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | ST50-05 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human Cytokeratin 7/13/17/18 aa 18-67. (SwissProt: P08729 Human; SwissProt: Q9DCV7 Mouse) |
| Localization | Cytoplasm. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | KRT7 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Choline Kinase beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Choline Kinase beta [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 7/13/17/18 Antibody (NBP2-67533)Find related products by research area.
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| Gene Symbol | KRT7 |
![Immunocytochemistry/Immunofluorescence: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Analysis of SKBR-3 cells labeling Cytokeratin 7. Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS for 10 minutes and blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with the primary antibodies Cytokeratin 7 (red) at 1/100 dilution for overnight at 4 . Goat anti rabbit IgG (iFluorTM 594) was used as the secondary antibody at 1/1,000 dilution dilution. DAPI was used as nuclear counterstain.](http://images.novusbio.com/fullsize/Cytokeratin-7-13-17-18-Antibody-ST50-05-Immunocytochemistry-Immunofluorescence-NBP2-67533-img0011.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Analysis of SKBR-3 cells labeling Cytokeratin 7. Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS for 10 minutes and blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with the primary antibodies Cytokeratin 7 (red) at 1/100 dilution for overnight at 4 . Goat anti rabbit IgG (iFluorTM 594) was used as the secondary antibody at 1/1,000 dilution dilution. DAPI was used as nuclear counterstain.")
![Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Cytokeratin 7 ( washed with PBS. And then probed with the primary antibodies Cytokeratin 7 (red) at 1/400 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-7-13-17-18-Antibody-ST50-05-Immunohistochemistry-Paraffin-NBP2-67533-img0016.jpg "Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Cytokeratin 7 ( washed with PBS. And then probed with the primary antibodies Cytokeratin 7 (red) at 1/400 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Flow Cytometry: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Flow cytometric analysis of Cytokeratin 7/13/17/18 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Cytokeratin-7-13-17-18-Antibody-ST50-05-Flow-Cytometry-NBP2-67533-img0008.jpg "Flow Cytometry: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Flow cytometric analysis of Cytokeratin 7/13/17/18 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Staining Cytokeratin 7 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-7-13-17-18-Antibody-ST50-05-Immunocytochemistry-Immunofluorescence-NBP2-67533-img0002.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Staining Cytokeratin 7 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Staining Cytokeratin 7 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-7-13-17-18-Antibody-ST50-05-Immunocytochemistry-Immunofluorescence-NBP2-67533-img0003.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Staining Cytokeratin 7 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Staining Cytokeratin 7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-7-13-17-18-Antibody-ST50-05-Immunocytochemistry-Immunofluorescence-NBP2-67533-img0004.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Staining Cytokeratin 7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 7 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-7-13-17-18-Antibody-ST50-05-Immunohistochemistry-Paraffin-NBP2-67533-img0005.jpg "Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 7 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 7 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-7-13-17-18-Antibody-ST50-05-Immunohistochemistry-Paraffin-NBP2-67533-img0006.jpg "Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 7 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Cytokeratin 7/13/17/18 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-62, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-7-13-17-18-Antibody-ST50-05-Immunohistochemistry-Paraffin-NBP2-67533-img0009.jpg "Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Cytokeratin 7/13/17/18 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-62, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Cytokeratin 7/13/17/18 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-62, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-7-13-17-18-Antibody-ST50-05-Immunohistochemistry-Paraffin-NBP2-67533-img0010.jpg "Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Cytokeratin 7/13/17/18 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-62, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-7-13-17-18-Antibody-ST50-05-Immunohistochemistry-Paraffin-NBP2-67533-img0012.jpg "Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Analysis of paraffin-embedded human breast tissue using anti-Cytokeratin 7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-7-13-17-18-Antibody-ST50-05-Immunohistochemistry-Paraffin-NBP2-67533-img0013.jpg "Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Analysis of paraffin-embedded human breast tissue using anti-Cytokeratin 7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Analysis of paraffin-embedded human placenta tissue using anti-Cytokeratin 7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Cytokeratin-7-13-17-18-Antibody-ST50-05-Immunohistochemistry-Paraffin-NBP2-67533-img0014.jpg "Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Analysis of paraffin-embedded human placenta tissue using anti-Cytokeratin 7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 7 (washed with PBS. And then probed with the primary antibodies Cytokeratin 7 (red) at 1/400 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-7-13-17-18-Antibody-ST50-05-Immunohistochemistry-Paraffin-NBP2-67533-img0015.jpg "Immunohistochemistry-Paraffin: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 7 (washed with PBS. And then probed with the primary antibodies Cytokeratin 7 (red) at 1/400 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Flow Cytometry: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Analysis of Hela cells with Cytokeratin 7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Cytokeratin-7-13-17-18-Antibody-ST50-05-Flow-Cytometry-NBP2-67533-img0001.jpg "Flow Cytometry: Cytokeratin 7/13/17/18 Antibody (ST50-05) [NBP2-67533] - Analysis of Hela cells with Cytokeratin 7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.")
![Immunohistochemistry Neprilysin/CD10 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neprilysin_AF1126_Immunohistochemistry_6679.jpg)
![Western Blot Neprilysin/CD10 Antibody [Unconjugated]](https://images.novusbio.com/images/af1126_mouse-neprilysin-affinity-purified-polyclonal-ab-8220249505976.jpg)
![Western Blot Neprilysin/CD10 Antibody [Unconjugated]](https://images.novusbio.com/images/af1126_mouse-neprilysin-affinity-purified-polyclonal-ab-44202415433122.jpg)
![Immunohistochemistry CEACAM7 Antibody [Unconjugated]](https://images.novusbio.com/images2/CEACAM7_AF4478_Immunohistochemistry_20616.jpg)
![Western Blot CEACAM7 Antibody [Unconjugated]](https://images.novusbio.com/images2/CEACAM-7_AF4478_Western_Blot_5475.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")