Reactivity | HuSpecies Glossary |
Applications | WB, IHC |
Clone | KRT20/3129R |
Clonality | Monoclonal |
Host | Rabbit |
Conjugate | Unconjugated |
Format | Azide and BSA Free |
Concentration | 1 mg/ml |
Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07634). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
Additional Information | Recombinant Monoclonal Antibody |
Immunogen | Recombinant fragment of human Cytokeratin 20 protein (around aa 196-323) (exact sequence is proprietary) (Uniprot: P35900) |
Localization | Cytoplasmic |
Marker | Colorectal Epithelial Marker |
Specificity | This monoclonal antibody recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20). KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions. It has been detected in adenocarcinomas of the colon, stomach and biliary tract. Breast carcinomas are generally non-reactive. |
Isotype | IgG |
Clonality | Monoclonal |
Host | Rabbit |
Gene | KRT20 |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. |
Theoretical MW | 46 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
Buffer | 10 mM PBS |
Preservative | No Preservative |
Concentration | 1 mg/ml |
Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 20 Antibody (NBP3-08636)Find related products by research area.
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Gene Symbol | KRT20 |