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Cytokeratin 20 Antibody (KRT20/3129R) - Azide and BSA Free

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Western Blot: Cytokeratin 20 Antibody (KRT20/3129R) - Azide and BSA Free [NBP3-08636] - Western Blot Analysis of HT29 cell lysate using Cytokeratin 20 Recombinant Rabbit Monoclonal Antibody (KRT20/3129R).
Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (KRT20/3129R) - Azide and BSA Free [NBP3-08636] - Formalin-fixed, paraffin-embedded human Colon stained with Cytokeratin 20 Recombinant Rabbit Monoclonal Antibody ...read more
Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (KRT20/3129R) - Azide and BSA Free [NBP3-08636] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 20 Recombinant Rabbit Monoclonal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
KRT20/3129R
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

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Cytokeratin 20 Antibody (KRT20/3129R) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07634).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant fragment of human Cytokeratin 20 protein (around aa 196-323) (exact sequence is proprietary) (Uniprot: P35900)
Localization
Cytoplasmic
Marker
Colorectal Epithelial Marker
Specificity
This monoclonal antibody recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20). KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions. It has been detected in adenocarcinomas of the colon, stomach and biliary tract. Breast carcinomas are generally non-reactive.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
KRT20
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Cytokeratin 20 Antibody (KRT20/3129R) - Azide and BSA Free

  • CD20
  • CK20
  • CK-20
  • cytokeratin-20
  • K20cytokeratin 20
  • keratin 20
  • keratin, type I cytoskeletal 20
  • keratin-20
  • KRT21
  • MGC35423
  • Protein IT

Background

Intermediate-sized filament (IF) protein designated cytokeratin (CK) 1 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract (1). Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium (2). CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol KRT20