![Western Blot: Cytokeratin 15 Antibody (KRT15/2959) - Azide and BSA Free [NBP3-08599] - Western Blot Analysis of Human Thymus tissue lysate using Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2959)](http://images.novusbio.com/fullsize/Cytokeratin-15-Antibody-KRT15-2959-Azide-and-BSA-Free-Western-Blot-NBP3-08599-img0004.jpg "Western Blot: Cytokeratin 15 Antibody (KRT15/2959) - Azide and BSA Free [NBP3-08599] - Western Blot Analysis of Human Thymus tissue lysate using Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2959)")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, MA |
| Clone | KRT15/2959 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07620). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant full-length human Cytokeratin 15 protein (Uniprot: P19012) |
| Localization | Cytoplasmic |
| Marker | Esophageal Squamous Cell Carcinoma Marker |
| Isotype | IgG2c Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT15 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry Frozen: & Formalin-fixed: 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. |
| Theoretical MW | 52 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
![Choline Kinase beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Choline Kinase beta [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 15 Antibody (NBP3-08599)Find related products by research area.
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| Gene Symbol | KRT15 |
![Immunocytochemistry/Immunofluorescence: Cytokeratin 15 Antibody (KRT15/2959) - Azide and BSA Free [NBP3-08599] - Confocal immunofluorescence image of MCF-7 cells using Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2959) followed by goat anti- Mouse CF488 (Cyan) and RedDot is used to label the nuclei Red.](http://images.novusbio.com/fullsize/Cytokeratin-15-Antibody-KRT15-2959-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP3-08599-img0001.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 15 Antibody (KRT15/2959) - Azide and BSA Free [NBP3-08599] - Confocal immunofluorescence image of MCF-7 cells using Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2959) followed by goat anti- Mouse CF488 (Cyan) and RedDot is used to label the nuclei Red.")
![Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (KRT15/2959) - Azide and BSA Free [NBP3-08599] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2959).](http://images.novusbio.com/fullsize/Cytokeratin-15-Antibody-KRT15-2959-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08599-img0003.jpg "Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (KRT15/2959) - Azide and BSA Free [NBP3-08599] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2959).")
![Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (KRT15/2959) - Azide and BSA Free [NBP3-08599] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2959)](http://images.novusbio.com/fullsize/Cytokeratin-15-Antibody-KRT15-2959-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08599-img0002.jpg "Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (KRT15/2959) - Azide and BSA Free [NBP3-08599] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2959)")
![Protein Array: Cytokeratin 15 Antibody (KRT15/2959) - Azide and BSA Free [NBP3-08599] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2959).](http://images.novusbio.com/fullsize/Cytokeratin-15-Antibody-KRT15-2959-Azide-and-BSA-Free-Protein-Array-NBP3-08599-img0005.jpg "Protein Array: Cytokeratin 15 Antibody (KRT15/2959) - Azide and BSA Free [NBP3-08599] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2959).")
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