![Western Blot: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Analysis of Cytokeratin 16 on human skin lysates using anti-Cytokeratin 16 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Cytokeratin-16-Antibody-SC52-09-Western-Blot-NBP2-67559-img0008.jpg "Western Blot: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Analysis of Cytokeratin 16 on human skin lysates using anti-Cytokeratin 16 antibody at 1/1,000 dilution.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | SC52-09 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within huamn Cytokeratin 16 aa 27-75 / 473. (SwissProt: P08779 Human) |
| Localization | Cytoskeleton, intermediate filament, cytosol, extracellular exosome, nucleus. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | KRT16 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Choline Kinase beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Choline Kinase beta [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 16 Antibody (NBP2-67559)Find related products by research area.
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| Gene Symbol | KRT16 |
![Immunocytochemistry/Immunofluorescence: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Staining Cytokeratin 16 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-16-Antibody-SC52-09-Immunocytochemistry-Immunofluorescence-NBP2-67559-img0004.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Staining Cytokeratin 16 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 16 with Rabbit anti-Cytokeratin 16 antibody washed with PBS, and then probed with the primary antibody (red) at 1/200 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-16-Antibody-SC52-09-Immunohistochemistry-Paraffin-NBP2-67559-img0010.jpg "Immunohistochemistry-Paraffin: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 16 with Rabbit anti-Cytokeratin 16 antibody washed with PBS, and then probed with the primary antibody (red) at 1/200 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Flow Cytometry: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Analysis of HepG2 cells with Cytokeratin 16 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Cytokeratin-16-Antibody-SC52-09-Flow-Cytometry-NBP2-67559-img0001.jpg "Flow Cytometry: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Analysis of HepG2 cells with Cytokeratin 16 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Staining Cytokeratin 16 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-16-Antibody-SC52-09-Immunocytochemistry-Immunofluorescence-NBP2-67559-img0002.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Staining Cytokeratin 16 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Staining Cytokeratin 16 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-16-Antibody-SC52-09-Immunocytochemistry-Immunofluorescence-NBP2-67559-img0003.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Staining Cytokeratin 16 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 16 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-16-Antibody-SC52-09-Immunohistochemistry-Paraffin-NBP2-67559-img0005.jpg "Immunohistochemistry-Paraffin: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 16 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Analysis of paraffin-embedded human lung cancer tissue using anti-Cytokeratin 16 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-16-Antibody-SC52-09-Immunohistochemistry-Paraffin-NBP2-67559-img0006.jpg "Immunohistochemistry-Paraffin: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Analysis of paraffin-embedded human lung cancer tissue using anti-Cytokeratin 16 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 16 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-16-Antibody-SC52-09-Immunohistochemistry-Paraffin-NBP2-67559-img0007.jpg "Immunohistochemistry-Paraffin: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 16 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Immunofluorescence analysis of paraffin-embedded human esophagus tissue labeling Cytokeratin 16 (washed with PBS. And then probed with the primary antibodies Cytokeratin 16 (red) at 1/200 dilution and Vimentin (green) at 1/400 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-16-Antibody-SC52-09-Immunohistochemistry-Paraffin-NBP2-67559-img0009.jpg "Immunohistochemistry-Paraffin: Cytokeratin 16 Antibody (SC52-09) [NBP2-67559] - Immunofluorescence analysis of paraffin-embedded human esophagus tissue labeling Cytokeratin 16 (washed with PBS. And then probed with the primary antibodies Cytokeratin 16 (red) at 1/200 dilution and Vimentin (green) at 1/400 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")