![Western Blot: Involucrin Antibody (SY5) [NB100-2727] - Involucrin from primary human epidermal keratinocyte lysates grown in low calcium (0.07 mM) for 5 days or differentiated with high calcium (1.5 mM) for 1-5 days. Block in 5% nonfat dry milk in TBST incubated at RT for 1 hr, antibody 1:500 in TBST, overnight at 4C. Image from verified customer review.](http://images.novusbio.com/fullsize/Involucrin-Antibody-SY5-Western-Blot-NB100-2727-img0003.jpg "Western Blot: Involucrin Antibody (SY5) [NB100-2727] - Involucrin from primary human epidermal keratinocyte lysates grown in low calcium (0.07 mM) for 5 days or differentiated with high calcium (1.5 mM) for 1-5 days. Block in 5% nonfat dry milk in TBST incubated at RT for 1 hr, antibody 1:500 in TBST, overnight at 4C. Image from verified customer review.")
| Reactivity | Hu, Po, Ca, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP, CyTOF-ready |
| Clone | SY5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified involucrin from human keratinocytes. |
| Epitope | The epitope maps between codon 421-568 of human involucrin. |
| Localization | Cytoplasmic |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | IVL |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Localized to upper spinous and granular layers in normal skin. IHC: Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37 degrees Celsius. Note than enzyme digestion is better than citrate buffer based epitope unmasking. This antibody is CyTOF ready. Positive control(s): MCF-7 cells. |
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| Theoretical MW | 118 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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Enlarge |
reviewed by:
Verified Customer |
WB | Human | 04/25/2018 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for Involucrin Antibody (NB100-2727)Find related products by research area.
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