MCF-7 Whole Cell Lysate Summary
| Specificity |
Total Protein - Human Tumor Cell Line: MCF 7 |
Applications/Dilutions
| Dilutions |
- Immunoprecipitation
- Western Blot
|
| Application Notes |
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles. |
| Buffer |
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors |
| Concentration |
5.0 mg/ml |
Lysate Details for Array
| Type |
Cell |
| Tissue |
Breast |
| Protein State |
Native |
| Subcellular Fraction |
Whole |
Background
Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are
guaranteed for 6 months from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for MCF-7 Lysate (NB820-59465). (Showing 1 - 1 of 1 FAQ).
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We would like to check toxicity of following products. For the following antibodies, could you inform us the concentration of sodium azide? Synaptojanin 1 Antibody (AC1) (cat# NB300-428), Ly-6C Antibody (HK1.4) [Biotin] (cat# NBP1-28048), and Cripto1 Antibody (cat# NB100-1598). For your MCF-7 Whole Cell Lysate (cat# NB820-59465), could you inform us the concentration of EDTA, Glycerol and NP-40?
- NB300-428 is supplied in 0.02% Sodium Azide NBP1-28048 contains 0.1% Sodium Azide and NB100-1598 has 0.05% sodium azide. With regards to your second question, unfortunately the formula for the lysis buffer is proprietary thus the component concentration is not suppose to be disclosed due to Novus policy.
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