![Immunocytochemistry/Immunofluorescence: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HOXB2 Antibody (PCRP-HOXB2-1F2). followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).](http://images.novusbio.com/fullsize/HOXB2-Antibody-PCRP-HOXB2-1F2-Immunocytochemistry-Immunofluorescence-NBP3-13937-img0002.jpg "Immunocytochemistry/Immunofluorescence: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HOXB2 Antibody (PCRP-HOXB2-1F2). followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, IP, MA |
| Clone | PCRP-HOXB2-1F2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14063) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. |
| Immunogen | Recombinant fragment (aa114-220) of human HOXB2 (Uniprot: P14652) |
| Localization | Nucleus, Nucleoplasm |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | HOXB2 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | ELISA: For coating, order antibody without BSA Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate) |
| Theoretical MW | 37.9 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
![SECTM1 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "SECTM1 [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - IHC analysis of formalin-fixed, paraffin-embedded human breast carcinoma. Nuclear staining using HOXB2 antibody (PCRP-HOXB2-1F2)at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/HOXB2-Antibody-PCRP-HOXB2-1F2-Immunohistochemistry-Paraffin-NBP3-13937-img0004.jpg "Immunohistochemistry-Paraffin: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - IHC analysis of formalin-fixed, paraffin-embedded human breast carcinoma. Nuclear staining using HOXB2 antibody (PCRP-HOXB2-1F2)at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.")
![Flow Cytometry: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - Flow cytometric analysis of PFA-fixed HeLa cells. HOXB2 antibody (PCRP-HOXB2-1F2) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/HOXB2-Antibody-PCRP-HOXB2-1F2-Flow-Cytometry-NBP3-13937-img0001.jpg "Flow Cytometry: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - Flow cytometric analysis of PFA-fixed HeLa cells. HOXB2 antibody (PCRP-HOXB2-1F2) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).")
![Immunohistochemistry-Paraffin: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - IHC analysis of formalin-fixed, paraffin-embedded human thyroid. Nuclear staining using HOXB2 antibody (PCRP-HOXB2-1F2)at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/HOXB2-Antibody-PCRP-HOXB2-1F2-Immunohistochemistry-Paraffin-NBP3-13937-img0003.jpg "Immunohistochemistry-Paraffin: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - IHC analysis of formalin-fixed, paraffin-embedded human thyroid. Nuclear staining using HOXB2 antibody (PCRP-HOXB2-1F2)at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.")
![Protein Array: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HOXB2 Antibody (PCRP-HOXB2-1F2).](http://images.novusbio.com/fullsize/HOXB2-Antibody-PCRP-HOXB2-1F2-Protein-Array-NBP3-13937-img0006.jpg "Protein Array: HOXB2 Antibody (PCRP-HOXB2-1F2) [NBP3-13937] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HOXB2 Antibody (PCRP-HOXB2-1F2).")
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