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Cytokeratin 10/13 Antibody (DE-K13) [Alexa Fluor® 647]

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Product Details

Summary
Reactivity Hu, FeSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, ICC/IF
Clone
DE-K13
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 647

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Cytokeratin 10/13 Antibody (DE-K13) [Alexa Fluor® 647] Summary

Immunogen
Cytoskeletal preparation extracted from human ectocervical epithelium
Localization
Cytoplasmic
Specificity
This antibody recognizes cytokeratin 10 (56.5kDa) and cytokeratin 13 (53kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this monoclonal antibody serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT10
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Cytokeratin 10/13 Antibody (DE-K13) [Alexa Fluor® 647]

  • BCIE
  • BIE
  • CK10
  • CK-10
  • cytokeratin 10
  • Cytokeratin-10
  • EHK
  • K10keratosis palmaris et plantaris
  • keratin 10
  • keratin, type I cytoskeletal 10
  • keratin-10
  • KPP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Cytokeratin 10/13 Products

Array NBP3-11497AF647

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Bioinformatics

Gene Symbol KRT10