We would like to use the CYBB (NOX2) antibody for Western blotting on rat heart samples. Please, suggest us an alternative solution, if you have any!

Name: CYBB/NOX2 Antibody
Brand: Novus Biologicals
SKU: NBP1-51928
Price: 399 USD

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Accepted Answer

I have taken a look for an alternative antibody, and we do have NBP1-51928 available. It has been validated for use in Western Blot however we have not yet tested this with Rat. I have carried out a BLAST on a protein database called UniProt and I have found that the percentage homology between the human CYBB and rat CYBB is 92.0%. If you would like to test this species you may find our Innovators Reward Program of interest, where we reward customers for trying our antibodies in novel species and applications.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, CaSpecies Glossary
Applications	ELISA, IHC
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	0.5 mg/ml

Immunogen	Peptide with sequence C-SYLNFARKRIKNP corresponding to internal region according to NP_000388.2.
Epitope	C-SYLNFARKRIKNP
Predicted Species	Canine (100%). Backed by our 100% Guarantee.
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	CYBB
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at -20C. Avoid freeze-thaw cycles.
Buffer	Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative	0.02% Sodium Azide
Concentration	0.5 mg/ml
Purity	Immunogen affinity purified

Gene Symbol	CYBB
Entrez	1536(Human)
Uniprot	NP_000388.2(Human)

CYBB/NOX2 Antibody

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