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CXCR7/RDC-1 Antibody - BSA Free

Images

 
Simple Western: CXCR7/RDC-1 Antibody [NBP2-24779] - Lane view shows a specific band for CXCR7 in 0.5 mg/mL of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunohistochemistry-Paraffin: CXCR7/RDC-1 Antibody [NBP2-24779] - Analysis of a FFPE tissue section of human tonsil using 5 ug/mL concentration of CXCR7/RDC-1 antibody. The staining was developed using HRP labeled ...read more
Immunohistochemistry-Paraffin: CXCR7/RDC-1 Antibody [NBP2-24779] - Analysis of a FFPE tissue section of human tonsil using 5 ug/mL concentration of CXCR7/RDC-1 antibody. The staining was developed using HRP labeled ...read more
Western Blot: CXCR7/RDC-1 Antibody [NBP2-24779] - Total protein from human spleen, tonsil, lymph node and mouse spleen was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Sh, Bv, Eq, PmSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, Flow (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

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CXCR7/RDC-1 Antibody - BSA Free Summary

Immunogen
A synthetic peptide corresponding amino acids 106-129 of human CXCR7/RDC1 was used as the immunogen, GenBank NP_064707.1.
Predicted Species
Bovine (100%), Equine (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACKR3
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1 - 2 ug/ml
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 5 - 10 ug/mL
  • Simple Western 1:100
  • Western Blot 4 - 6 ug/mL
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by size.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-24779 in the following applications:

Publications
Read Publications using
NBP2-24779 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Peptide affinity purified

Alternate Names for CXCR7/RDC-1 Antibody - BSA Free

  • ACKR3
  • chemokine (C-X-C motif) receptor 7
  • Chemokine orphan receptor 1CMKOR1C-X-C chemokine receptor type 7
  • CMKOR1
  • CXCR7
  • CXC-R7
  • CXCR-7
  • G protein-coupled receptor
  • GPR159
  • GPR159G-protein coupled receptor 159
  • RDC-1
  • RDC1G-protein coupled receptor RDC1 homolog

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-24779
Species: Hu, Mu, Rt, Sh, Bv, Eq, Pm
Applications: WB, Simple Western, ICC/IF, IHC, Flow (-)

Publications for CXCR7/RDC-1 Antibody (NBP2-24779)(9)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Sheep.

We have publications tested in 6 applications: Flow Cytometry, Flow-CS, IHC, Immunohistochemistry, Immunohistochemistry-Frozen, WB.


Filter By Application
Flow Cytometry
(1)
Flow-CS
(1)
IHC
(1)
Immunohistochemistry
(1)
Immunohistochemistry-Frozen
(1)
WB
(4)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
Rat
(2)
Sheep
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using NBP2-24779 Applications Species
Wang Q, Xu S, Wang B et al. Chemokine receptor 7 mediates miRNA-182 to regulate cerebral ischemia/reperfusion injury in rats CNS neuroscience & therapeutics 2022-12-15 [PMID: 36523152] (IHC, WB, Rat)

Details:
Dilution used in WB 1:100, IHC 1:200
IHC, WB Rat
Sarah A Reed, Ryan Ashley, Gail Silver, Caitlyn Splaine, Amanda K Jones, Sambhu M Pillai, Maria L Peterson, Steven A Zinn, Kristen E Govoni Maternal nutrient restriction and over-feeding during gestation alter expression of key factors involved in placental development and vascularization. Journal of animal science 2022-06-03 [PMID: 35648126]
Lyu C, Ye Y, Lensing MM et al. Targeting Gi/o protein-coupled receptor signaling blocks HER2-induced breast cancer development and enhances HER2-targeted therapy JCI Insight 2021-09-22 [PMID: 34343132] (Immunohistochemistry) Immunohistochemistry
Ghadge SK, Messner M, Seiringer H et al. Smooth Muscle Specific Ablation of CXCL12 in Mice Downregulates CXCR7 Associated with Defective Coronary Arteries and Cardiac Hypertrophy International Journal of Molecular Sciences 2021-05-31 [PMID: 34072818] (Flow Cytometry, Immunohistochemistry-Frozen) Flow Cytometry, Immunohistochemistry-Frozen
Quinn K, Prosser S, Kane K et al. Inhibition of chemokine (C-X-C motif) receptor four (CXCR4) at the fetal-maternal interface during early gestation in sheep: alterations in expression of chemokines, angiogenic factors and their receptors. J Anim Sci 2017-03-28 [PMID: 28380526] (WB, Sheep) WB Sheep
Hattermann K, Held-Feindt J, Lucius R et al. The chemokine receptor CXCR7 is highly expressed in human glioma cells and mediates antiapoptotic effects. Cancer Res. 2010-04-15 [PMID: 20388803] (WB, Human)

Details:
WB (Fig 2): human glioma cell lines UC118, A739, A761, A764, A767, and A777. CXCR7 was detected at 50 kDa.
WB Human
Odemis V, Boosmann K, Heinen A et al. CXCR7 is an active component of SDF-1 signalling in astrocytes and Schwann cells. J Cell Sci. 2010-04-01 [PMID: 20197403] (WB, Rat)

Details:
WB: Fig 1C (primary rat astrocytes transfected with CXCR7 siRNA or left untreated. Fig 1D (primary rat Schwann cells); Fig 1G (primary mouse glial and rat Schwann cells). Flow (cell surface): Figs 1E & F (primary rat astrocytes), Figs 2A & B (primary rat
WB Rat
Zhang Z, Zhong W, Hall MJ et al. CXCR4 but not CXCR7 is mainly implicated in ocular leukocyte trafficking during ovalbumin-induced acute uveitis. Exp Eye Res. 2009-10-01 [PMID: 19524567] (Mouse)

Details:
FA (neutralization): In vivo FA assay: D011.10 mice [(intraperitoneal injection of 15 ug or intravitreal injection of 1.5 ug of CXCR7 pAb (IMG-6099A)], Fig 8. The mitigation of OVA-induced uveitis was assessed. The antibody did not significantly alter OVA
Mouse
Choy JC, Yi T, Rao DA et al. CXCL12 induction of inducible nitric oxide synthase in human CD8 T cells. J Heart Lung Transplant. 2008-12-01 [PMID: 19059114] (Flow-CS, Human)

Details:
Flow (cell surface): freshly isolated human CD4 and CD8 cells (Fig 1) and HeLa cells (data described, but not shown). CXCR7 was detected in HeLa but not in CD4 or CD8 cells.
Flow-CS Human

Review for CXCR7/RDC-1 Antibody (NBP2-24779) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-24779:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot CXCR7/RDC-1 NBP2-24779
Enlarge
5
reviewed by:
Verified Customer
WB Human 03/07/2020
View

Summary

ApplicationWestern Blot
Sample TestedT47D human breast cancer cell line
SpeciesHuman
LotC-2

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CXCR7/RDC-1 Antibody (NBP2-24779) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Verified Customer
03/07/2020
Application: WB
Species: Human

Bioinformatics

Gene Symbol ACKR3
Uniprot