Home » CXCR7/RDC-1 » CXCR7/RDC-1 Antibodies » CXCR7/RDC-1 Antibody (358426) [Unconjugated]

CXCR7/RDC-1 Antibody (358426) [Unconjugated]

 (8 Publications)
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CXCR7 was detected in immersion fixed paraffin-embedded sections of human breast tumor using Mouse Anti-Human CXCR7 Monoclonal Antibody (Catalog # MAB4227) at 15ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder.
CXCR7 was detected in immersion fixed paraffin-embedded sections of human breast tumor using Mouse Anti-Human CXCR7 Monoclonal Antibody (Catalog # MAB4227) at 15ug/mL at 37 ° Celsius for 4 minutes. Before incubation ...read more
Human peripheral blood monocytes were stained with Mouse Anti-Human CXCR7/RDC-1 Monoclonal Antibody (Catalog # MAB4227, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by ...read more
CXCR7/RDC-1 was detected in perfusion fixed paraffin-embedded sections of nude mice injected with human breast cancer cells using Mouse Anti-Human CXCR7/RDC-1 Monoclonal Antibody (Catalog # MAB4227) at 25 µg/mL ...read more
CXCR7/RDC-1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human CXCR7/RDC-1 Monoclonal Antibody (Catalog # MAB4227) at 0.5 µg/mL for 1 hour at room ...read more
Knockdown effect of MD-2 on exposure of TLR4 to LPS in SW480 and Colo 205 cell lines.A, SW480 and Colo 205 cell lines were transfected transiently with siRNA or negative control sequence(NC). SW480 and Colo 205 cell ...read more
Representative examples of immunohistochemical staining of TLR4, MD-2, and CXCR7 in colorectal carcinoma tissues (original magnification 100×).Positive staining was observed as a dark brown color. Normal colorectal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, CyTOF-ready, mIF
Clone
358426
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB4227U-100UG
Alexa Fluor 405
FAB4227V-100UG
Alexa Fluor 488
FAB4227G-100UG
Alexa Fluor 532
FAB4227X
Alexa Fluor 594
FAB4227T-100UG
Alexa Fluor 647
FAB4227R-100UG
Alexa Fluor 700
FAB4227N-100UG
Alexa Fluor 750
FAB4227S-100UG
Allophycocyanin/Cy7
FAB4227APCCY7
Biotin
FAB4227B
CoraFluor 1
FAB4227CL1
DyLight 350
FAB4227D
DyLight 405
FAB4227E
DyLight 488
FAB4227K
DyLight 550
FAB4227L
DyLight 594
FAB4227M
DyLight 650
FAB4227W
DyLight 680
FAB4227Y
DyLight 755
FAB4227Z
HRP
FAB4227H
Janelia Fluor 525
FAB4227JF525
Janelia Fluor 549
FAB4227I
Janelia Fluor 646
FAB4227J
Janelia Fluor 669
FAB4227JF669
PE/Atto594
FAB4227PEATT594
PE/Cy5.5
FAB4227PECY55
PE/Cy7
FAB4227PECY7
mFluor Violet 450 SE
FAB4227MFV450
mFluor Violet 500 SE
FAB4227MFV500
mFluor Violet 610 SE
FAB4227MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB4227-SP
Carrier Free
FAB4227S-100UG
Carrier Free
FAB4227R-100UG
Carrier Free
FAB4227N-100UG
Carrier Free
FAB4227V-100UG
Carrier Free
FAB4227U-100UG
Carrier Free
FAB4227T-100UG
Carrier Free
FAB4227G-100UG
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CXCR7/RDC-1 Antibody (358426) [Unconjugated] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human CXCR7/RDC‑1
Met1-Lys362 (Gly131Ser)
Accession # AAA62370
Specificity
Detects human CXCR7/RDC‑1 in direct ELISAs. In flow cytometry, reacts specifically with five distinct human CXCR7 transfectants, but does not react with their respective parental lines or mouse CXCR7 transfectants. In flow cytometry, also reacts with monocytes expressing CXCR7, but does not react with MCF-7 cells which have been reported to have surface-expressing CXCR7 using clone 11G8. Due to the conflicting reports published, use of monoclonal MAB4227 may result in an underestimation of CXCR7 expression on certain cell types.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CXCR7
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Multiplex Immunofluorescence 15 ug/mL
Publications
Read Publications using
MAB4227 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR7/RDC-1 Antibody (358426) [Unconjugated]

  • ACKR3
  • chemokine (C-X-C motif) receptor 7
  • Chemokine orphan receptor 1CMKOR1C-X-C chemokine receptor type 7
  • CMKOR1
  • CXCR7
  • CXC-R7
  • CXCR-7
  • G protein-coupled receptor
  • GPR159
  • GPR159G-protein coupled receptor 159
  • RDC-1
  • RDC1G-protein coupled receptor RDC1 homolog

Background

The G protein-coupled receptor, RDC1, belongs to a subgroup of chemokine receptors and has been designated CXCR7. CXCR7 can bind with high-affinity to CXCL12/SDF-1 and CXCL11/I-TAC. It is also a co-receptor for several HIV and SIV strains. In their N-termini and extracellular loops 1, 2, and 3, human and mouse CXCR7 share 84%, 100%, 96% and 86% amino acid sequence identity, respectively. Reports of mRNA levels and/or protein expression (as assessed using anti‑CXCR7, clone 9C4) (1, 2) indicate that CXCR7 occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. In contrast, based on ligand binding analysis and receptor level (as assessed using anti‑CXCR7, clone 11G8), surface expression of CXCR7 was reported to be restricted to tumor cells, activated endothelial cells, fetal liver cells, and few other cell types (3). The basis of these inconsistent observations is not known but may be attributed to cell context and the use of different antibodies that may recognize different epitopes.

  1. Balabanian, K. et al. (2005) J. Biol. Chem. 280:35760.
  2. Infantino, S. et al. (2006) J. Immunol. 176:2197.
  3. Burns, J.M. et al. (2006) J. Exp. Med. 203:2201.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CXCR7/RDC-1 Antibody (358426) [Unconjugated]
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Publications for CXCR7/RDC-1 Antibody (MAB4227)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Flow Cytometry, ICC, Western Blot.


Filter By Application
Flow Cytometry
(3)
ICC
(1)
Western Blot
(1)
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Human
(4)
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Showing Publications 1 - 8 of 8.
Publications using MAB4227 Applications Species
Nan Li, Huanbai Xu, Yurong Ou, Zhenzhong Feng, Qiong Zhang, Qing Zhu et al. LPS-induced CXCR7 expression promotes gastric Cancer proliferation and migration via the TLR4/MD-2 pathway Diagnostic Pathology 12/1/2019 [PMID: 30636642]
N Montpas, G St-Onge, N Nama, D Rhainds, B Benredjem, M Girard, G Hickson, V Pons, N Heveker Ligand-specific conformational transitions and intracellular transport required for atypical chemokine receptor 3-mediated chemokine scavenging J. Biol. Chem., 2017-11-27;0(0):. 2017-11-27 [PMID: 29180449] (Flow Cytometry, Human) Flow Cytometry Human
TIAN TANG, QINGJIE XIA, MINGRONG XI CXC chemokine receptor 7 expression in cervical intraepithelial neoplasia Biomedical Reports 1/1/2016 [PMID: 26870336]
Linhui Wang, Wei Chen, Li Gao, Qing Yang, Bing Liu, Zhenjie Wu et al. High expression of CXCR4, CXCR7 and SDF-1 predicts poor survival in renal cell carcinoma World Journal of Surgical Oncology 12/1/2012 [PMID: 23039915]
Huanbai Xu, Qiong Wu, Shipeng Dang, Min Jin, Jingwei Xu, Yiji Cheng et al. Alteration of CXCR7 Expression Mediated by TLR4 Promotes Tumor Cell Proliferation and Migration in Human Colorectal Carcinoma PLoS ONE 12/13/2011 [PMID: 22180778]
Takao T, Asanoma K, Kato K Isolation and characterization of human trophoblast side-population (SP) cells in primary villous cytotrophoblasts and HTR-8/SVneo cell line. PLoS ONE, 2011-07-07;6(7):e21990. 2011-07-07 [PMID: 21760941] (ICC, Western Blot, Human) ICC, Western Blot Human
Cabezon R, Sintes J, Llinas L, Benitez-Ribas D Analysis of HLDA9 mAbs on plasmacytoid dendritic cells. Immunol. Lett., 2010-10-07;134(2):167-73. 2010-10-07 [PMID: 20933012] (Flow Cytometry, Human) Flow Cytometry Human
Berahovich RD, Zabel BA, Penfold ME, Lewen S, Wang Y, Miao Z, Gan L, Pereda J, Dias J, Slukvin II, McGrath KE, Jaen JC, Schall TJ CXCR7 protein is not expressed on human or mouse leukocytes. J. Immunol., 2010-10-01;185(9):5130-9. 2010-10-01 [PMID: 20889540] (Flow Cytometry, Human) Flow Cytometry Human

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Bioinformatics

Gene Symbol CXCR7
Uniprot