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CXCR4 Antibody (247506R) [Unconjugated]

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HEK293 human embryonic kidney cell line transfected with mouse CXCR4 and eGFP was stained with (A) Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651R) or (B) Rat IgG2B isotype control antibody (Catalog # ...read more
Mouse thymocytes were stained with Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651R, filled histogram) or Rat IgG2B isotype control antibody (Catalog # MAB0061, open histogram) followed by Goat anti-Rat IgG ...read more
CXCR4 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651R) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the ...read more
CXCR4 was detected in perfusion fixed frozen sections of mouse spleen using Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651R) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB ...read more
CXCR4 was detected in perfusion fixed frozen sections of mouse testis using Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651R) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, CyTOF-ready, ICC/IF
Clone
247506R
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

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CXCR4 Antibody (247506R) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Y3 rat myeloid cell line transfected with mouse CXCR4
Met1-Ser359
Accession # P70658
Specificity
Detects mouse CXCR4 transfectants. Does not stain irrelevant transfectants.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 1-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR4 Antibody (247506R) [Unconjugated]

  • CD184
  • chemokine (C-X-C motif) receptor 4
  • chemokine (C-X-C motif), receptor 4 (fusin)
  • C-X-C chemokine receptor type 4
  • CXCR4
  • CXC-R4
  • CXCR-4
  • D2S201E
  • D2S201ESDF-1 receptor
  • FB22
  • Fusin
  • HM89
  • HM89NPYRL
  • HSY3RR
  • LAP3
  • LAP-3
  • LCR1
  • LESTR
  • LESTRCD184 antigen
  • Leukocyte-derived seven transmembrane domain receptor
  • leukocyte-derived seven-transmembrane-domain receptor
  • lipopolysaccharide-associated protein 3
  • neuropeptide Y receptor Y3
  • NPY3R
  • NPY3RWHIM
  • NPYR
  • NPYRL
  • NPYY3R
  • pCXCR4
  • seven transmembrane helix receptor
  • seven-transmembrane-segment receptor, spleen
  • Stromal cell-derived factor 1 receptor
  • WHIMS

Background

CXCR4, also known as CD184, is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells. While primarily a membrane protein, CXCR4 undergoes trafficking and internalization in response to stimulation with phorbol esters and ligand (1). Cytoplasmic and nuclear localization of CXCR4 has been observed in colorectal and renal carcinomas (2,3) and it has been used as the basis of prognosis and metastatic state (3,4,5).
  1. Orsini, M.J. et al. (1999) J. Biol. Chem. 274:31076.
  2. Zagzag, D. et al. (2005) Cancer Res. 65:6178.
  3. Speetjens, F.M. et al. (2009) Cancer Microenvironment 2:1.
  4. Wang, L. et al. (2009) Oncology Reports 22:1333.
  5. Amara, S. et al. (2015) Cancer Biomark. 15:869.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for CXCR4 Antibody (MAB21651R). (Showing 1 - 1 of 1 FAQ).

  1. Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.
    • CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

Secondary Antibodies

 

Isotype Controls

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LAMP2 belongs to the family of membrane glycoproteins who confer selectins with carbohydrate ligands. LAMP2 has been implicated in tumor cell metastasis, as well as overall protection, maintenance, and adhesion of the lysosome. It appears that LAMP2 m...  Read full blog post.

CXCR4 Studies on Neural and Stem Cells
The CXCR4 (C-X-C chemokine receptor type 4) protein is one member of the G-protein coupled receptor (GPCR1) family. As a multipass membrane protein that is found in several tissues, it is the receptor for the C-X-C chemokine CXCL12/SDF-1. The CXCR4 li...  Read full blog post.

Understanding CXCR4 and SDF1
CXCR4 (C-X-C chemokine receptor type 4) is a member of the G-protein coupled receptor (GPCR1) family. It is expressed as a multipass membrane protein in several tissues where it acts as the receptor for the C-X-C chemokine CXCL12/SDF-1. This ligand in...  Read full blog post.

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