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Recombinant Rat CXCL3/GRO gamma/CINC-2/DCIP-1 Protein

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier-Free
NBP2-35251-10ug
Carrier-Free
NBP2-35251-1mg
Carrier-Free
NBP2-35251-500ug
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Recombinant Rat CXCL3/GRO gamma/CINC-2/DCIP-1 Protein Summary

Description
A single non-glycosylated polypeptide chain containing 69 amino acids corresponding to CXCL3/GRO gamma/CINC-2/DCIP-1 Source: E. coli

Uniprot ID: Q10746

Amino Acid Sequence: RELRCQCLKT LPRVDFENIQ SLTVTPPGPH CTQTEVIATL KDGQEVCLNP QAPRLQKIIQ KLLKSDKSS

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Details of Functionality
CXCL3 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected murine BaF3 cells is in a concentration range of 5-50 ng/ml.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
CXCL3
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of CXCL3/GRO gamma/CINC-2/DCIP-1 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
7.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant Rat CXCL3/GRO gamma/CINC-2/DCIP-1 Protein

  • chemokine (C-X-C motif) ligand 3
  • CINC-2 beta
  • CINC-2 beta/CXCL3
  • CINC-2
  • CINC-2b
  • C-X-C motif chemokine 3
  • CXCL3
  • Dcip1
  • DCIP-1
  • DCIP-1/CXCL3
  • Gm1960
  • GRO gamma
  • GRO3 oncogene
  • GRO3
  • GROG
  • GRO-gamma
  • GRO-gamma(1-73)
  • Growth-regulated protein gamma
  • Macrophage inflammatory protein 2-beta
  • melanoma growth stimulatory activity gamma
  • MGSA gamma
  • MIP2B
  • MIP-2b
  • MIP2-beta
  • SCYB3

Background

CXCL3 is belonging to the CXC chemokine family, which is also known as CINC-2 in rat, DCIP-1 in murine, and GRO gamma in humans. The functional receptor for CXCL3 has been identified as CXCR2. Similar to other GRO proteins, CXCL3 is potent neutrophil attractants and activators. This chemokine were originally purified from the conditioned medium of rat granulation tissue. In rat, CINC-2 has two isoforms, known as CXCL3alpha/CINC-2alpha and CXCL3beta/CINC-2beta. CXCL3alpha differs from CXCL3beta as DKSS to PSL (98-101) at carboxy-terminal residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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NBP2-35251
Recombinant Rat CXCL3/GRO gamma/CINC-2/DCIP-1 Protein
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Bioinformatics

Gene Symbol CXCL3
Uniprot