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Recombinant Mouse CXCL3/GRO gamma/CINC-2/DCIP-1 Protein

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HPLC: Recombinant Mouse CXCL3/GRO gamma/CINC-2/DCIP-1 Protein [NBP2-35173] - Mouse CXCL3 Protein [NBP2-35173]

Product Details

Summary
Reactivity MuSpecies Glossary
Applications PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

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Recombinant Mouse CXCL3/GRO gamma/CINC-2/DCIP-1 Protein Summary

Description
A single, non-glycosylated polypeptide chain containing 73 amino acids corresponding to CXCL3/GRO gamma/CINC-2/DCIP-1 Source: E. coli

Uniprot ID: Q6W5C0

Amino Acid Sequence: AVVASELRCQ CLNTLPRVDF ETIQSLTVTP PGPHCTQTEV IATLKDGQEV CLNPQGPRLQ IIIKKILKSG KSS

Details of Functionality
CXCL3 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is in a concentration range of 10-100 ng/ml.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
CXCL3
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of CXCL3/GRO gamma/CINC-2/DCIP-1 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
7.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative
No Preservative
Concentration
LYOPH
Purity
> 97 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Mouse CXCL3/GRO gamma/CINC-2/DCIP-1 Protein

  • chemokine (C-X-C motif) ligand 3
  • CINC-2 alpha
  • CINC-2 alpha/CXCL3
  • CINC-2 beta
  • CINC-2 beta/CXCL3
  • CINC-2
  • CINC-2b
  • C-X-C motif chemokine 3
  • CXCL3
  • Dcip1
  • Gm1960
  • GRO gamma
  • GRO3 oncogene
  • GRO3
  • GROG
  • GRO-gamma
  • GRO-gamma(1-73)
  • Growth-regulated protein gamma
  • Macrophage inflammatory protein 2-beta
  • melanoma growth stimulatory activity gamma
  • MGSA gamma
  • MIP2B
  • MIP-2b
  • MIP2-beta
  • SCYB3

Background

CXCL3 is belonging to the CXC chemokine family, which is also known as CINC-2 in rat, DCIP-1 in murine, and GRO gamma in humans. The functional receptor for CXCL3 has been identified as CXCR2. Similar to other GRO proteins, CXCL3 is potent neutrophil attractants and activators. This chemokine were originally purified from the conditioned medium of rat granulation tissue. In rat, CINC-2 has two isoforms, known as CXCL3alpha/CINC-2alpha and CXCL3beta/CINC-2beta. CXCL3alpha differs from CXCL3beta as DKSS to PSL (98-101) at carboxy-terminal residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CXCL3