Recombinant Mouse CXCL3/GRO gamma/CINC-2/DCIP-1 Protein Summary
Description |
A single, non-glycosylated polypeptide chain containing 73 amino acids corresponding to CXCL3/GRO gamma/CINC-2/DCIP-1 Source: E. coli Uniprot ID: Q6W5C0 Amino Acid Sequence: AVVASELRCQ CLNTLPRVDF ETIQSLTVTP PGPHCTQTEV IATLKDGQEV CLNPQGPRLQ IIIKKILKSG KSS |
Details of Functionality |
CXCL3 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is in a concentration range of 10-100 ng/ml. |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
CXCL3 |
Purity |
> 97 % pure by SDS-PAGE and HPLC |
Endotoxin Note |
Less than 1 EU/ug of CXCL3/GRO gamma/CINC-2/DCIP-1 as determined by LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
7.9 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Buffer |
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
Preservative |
No Preservative |
Concentration |
LYOPH |
Purity |
> 97 % pure by SDS-PAGE and HPLC |
Reconstitution Instructions |
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
Notes
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Alternate Names for Recombinant Mouse CXCL3/GRO gamma/CINC-2/DCIP-1 Protein
Background
CXCL3 is belonging to the CXC chemokine family, which is also known as CINC-2 in rat, DCIP-1 in murine, and GRO gamma in humans. The functional receptor for CXCL3 has been identified as CXCR2. Similar to other GRO proteins, CXCL3 is potent neutrophil attractants and activators. This chemokine were originally purified from the conditioned medium of rat granulation tissue. In rat, CINC-2 has two isoforms, known as CXCL3alpha/CINC-2alpha and CXCL3beta/CINC-2beta. CXCL3alpha differs from CXCL3beta as DKSS to PSL (98-101) at carboxy-terminal residues.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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