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Ctip1 Antibody (CL13530)

Images

 
Staining in NTERA-2 cell line with Anti-BCL11A monoclonal antibody, showing specific staining of nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Staining of human cervix shows strong nuclear positivity in squamous epithelial cells.
Staining of human tonsil shows strong nuclear positivity in germinal center cells.
Orthogonal Strategies: Western blot analysis in human cell lines HEK293 and A-549 using Anti-BCL11A antibody. Corresponding BCL11A RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Orthogonal Strategies: Analysis in human cerebral cortex and skeletal muscle tissues using NBP3-24563 antibody. Corresponding BCL11A RNA-seq data are presented for the same tissues.
Staining of human skeletal muscle shows no positivity in myocytes as expected.
Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL13530
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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Ctip1 Antibody (CL13530) Summary

Immunogen
This antibody was designed to target the recombinant protein Ctip1, with the precise immunogen sequence being withheld for proprietary reasons.
Predicted Species
Mouse (92%), Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BCL11A
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Ctip1 Antibody (CL13530)

  • B-cell CLL/lymphoma 11A (zinc finger protein) isoform 2
  • B-cell CLL/lymphoma 11A (zinc finger protein)
  • B-cell CLL/lymphoma 11A
  • B-cell lymphoma/leukemia 11A
  • BCL11A B-cell CLL/lymphoma 11A (zinc finger protein) isoform 1
  • BCL-11A
  • BCL11A-S
  • BCL11A-XL
  • C2H2-type zinc finger protein
  • COUP-TF-interacting protein 1
  • CTIP1ecotropic viral integration site 9
  • ecotropic viral integration site 9 homolog
  • Ecotropic viral integration site 9 protein homolog
  • EVI-9
  • EVI9FLJ34997
  • HBFQTL5
  • KIAA1809BCL11A-L
  • Zinc finger protein 856
  • ZNF856FLJ10173

Background

The Ctip1 gene encodes a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. The corresponding mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, throug

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol BCL11A