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CSE1L/CAS/Exportin-2 Antibody

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Biological Strategies: Western Blot: CSE1L/CAS/Exportin-2 Antibody [NB100-79803] - Detection of Human and Mouse CSE1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells ...read more
Immunohistochemistry-Paraffin: CSE1L/CAS/Exportin-2 Antibody [NB100-79803] - Mouse plasmacytoma. Antibody: Affinity purified rabbit anti-CSE1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: CSE1L/CAS/Exportin-2 Antibody [NB100-79803] - Human testicular seminoma. Antibody: Affinity purified rabbit anti-CSE1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunoprecipitation: CSE1L/CAS/Exportin-2 Antibody [NB100-79803] - Samples: A. Whole cell lysate from HeLa (5, 15 and 50 mcg) and Ramos (R; 50 mcg) cells. B. Whole cell lysate (1 mg for IP, 1/4 of IP loaded) from HeLa ...read more
Independent Antibodies: Immunoprecipitation: CSE1L/CAS/Exportin-2 Antibody [NB100-79803] - Detection of human CSE1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; ...read more

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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CSE1L/CAS/Exportin-2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Chromosome Segregation-1 using the numbering given in entry NP_001307.2 (GeneID 1434).
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CSE1L
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:10000-1:25000
Application Notes
Immunohistochemistry 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CSE1L/CAS/Exportin-2 Antibody

  • CAS
  • CASMGC117283
  • Cellular apoptosis susceptibility protein
  • chromosome segregation 1 (yeast homolog)-like
  • Chromosome segregation 1-like protein
  • CSE1 chromosome segregation 1-like (yeast)
  • CSE1
  • CSE1L
  • exp2
  • exportin-2
  • Importin-alpha re-exporter
  • MGC130037
  • XPO2
  • XPO2MGC130036

Background

Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex. After translocation, RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind. The protein encoded by this gene binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition, the encoded protein may play a role both in apoptosis and in cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CSE1L
Entrez
Uniprot