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Coagulation Factor II/Thrombin Antibody (200710) [Unconjugated] - Pro form

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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Pro Coagulation Factor II/Thrombin Monoclonal Antibody (Catalog # MAB1473) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Pro Coagulation Factor II/Thrombin at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Pro Coagulation Factor II/Thrombin Monoclonal Antibody (Catalog # MAB1473) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clone
200710
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB1473-SP
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Coagulation Factor II/Thrombin Antibody (200710) [Unconjugated] - Pro form Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Pro Coagulation Factor II/Thrombin
Ala44-Glu622
Accession # NP_000497
Specificity
Detects human Pro Coagulation Factor II/Thrombin in direct ELISAs and Western blots. It recognizes only Pro Coagulation Factor II/Thrombin, not active Thrombin. In Western blots, less than 5% cross-reactivity with recombinant human (rh) Factor X, rhKallikrein 1, 3, 5, and 11 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
F2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunoprecipitation 25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Coagulation Factor II/Thrombin Antibody (200710) [Unconjugated] - Pro form

  • coagulation factor II (thrombin) receptor-like 2
  • Coagulation factor II receptor-like 2 (protease-actovated receptor 3)
  • Coagulation factor II receptor-like 2
  • Coagulation Factor II
  • F2
  • PAR-3
  • PAR3proteinase-activated receptor 3
  • protease-activated receptor 3
  • proteinase-activated receptor-3
  • PT
  • serine protease
  • Thrombin receptor-like 2
  • Thrombin

Background

Pro-Coagulation Factor II, also called Prothrombin, is a plasma serine protease central to the coagulation cascade in which it converts fibrinogen to fibrin, activates factors V, VII, VIII, XIII and forms complexes with protein C and thrombomodulin. Pro-Coagulation Factor II is an inactive precursor of thrombin that can be activated by proteolytic removal of the pro region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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FAQs for Coagulation Factor II/Thrombin Antibody (MAB1473). (Showing 1 - 1 of 1 FAQ).

  1. Do you have neutralizing antibodies for anti-mouse Thrombin?
    • We currently have 1 neutralizing Thrombin antibody (NBP1-95029), however it is an anti-human antibody and has only been validated in human samples. If you would like to test this antibody in mouse samples, you may be interested in participating in our Innovators Reward Program.

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Bioinformatics

Gene Symbol F2
Uniprot